Journal of Materials Science: Materials in Medicine

, Volume 14, Issue 9, pp 731–738 | Cite as

The effect of particle size and electrical charge on macrophage-osteoclast differentiation and bone resorption

Article

Abstract

In aseptic loosening, there is commonly periprosthetic bone loss and a heavy macrophage infiltrate in response to biomaterial wear particles generated from the implant materials. Macrophages which have phagocytosed wear particles are known to be capable of differentiation into bone resorbing osteoclasts. In this investigation we determine the role of particle size and particle charge on this process. Mouse monocytes and macrophages were co-cultured with osteoblast-like UMR106 cells and 1,25 dihydroxyvitamin D3 in the presence or the absence of (i) various sizes of latex beads (0.1, 1, 10 and 100 μm) and (ii) uncharged, positively- or negatively-charged sephadex beads of uniform shape and composition. The extent of osteoclast differentiation by monocytes or foreign body macrophages was determined by the expression of the osteoclast-associated enzyme tartrate-resistant acid phosphatase and lacunar bone resorption. No significant difference in the extent of osteoclast formation and bone resorption was noted in response to particle size. Osteoclast formation was also not significantly different in the presence of positively/negatively charged and uncharged particles. These findings indicate that osteoclast formation is not significantly influenced by particle characteristics, such as particle size. They also add support to the hypothesis that macrophage involvement in periprosthetic osteolysis is not dependent on particle phagocytosis and that it may be induced by particle contact.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    T. M. Wright and S. B. Goodman (eds), “Implant Wear: The Future of Total Joint Replacements” (Oakbrook, AAOS, 1995).Google Scholar
  2. 2.
    W. J. Maloney, J. Bone Joint Surg. 77A (1995) 1448.Google Scholar
  3. 3.
    P. A. Revell, in “Current Trends in Pathology”, edited by C. Berry (Berlin, Springer-Verlag: 1982) p. 73.Google Scholar
  4. 4.
    S. B. Goodman, R. C. Chris and S. S. Chiou, Clin. Orthop. 244 (1989) 182.Google Scholar
  5. 5.
    W. A. Jiranek, M. Machado, M. Jasty, D. Jervevar, H. J. Wolfe, S. R. Goldring, M. J. Goldberg and W. H. Harris, J. Bone Joint Surg. 75A (1993) 863.Google Scholar
  6. 6.
    T. T. Glant, J. J. Jacobs, K. Mikecz, J. Yao, S. Chubinskaja, J. M. Williams, R. L. Urban, A. S. Shahbang, S. H. Lee and D. R. Sumner, Am. J. Ther. 3 (1996) 27.Google Scholar
  7. 7.
    J. Chiba, H. E. Rubash, K. J. Kim and Y. Iwaki, Clin. Orthop. 300 (1994) 304.Google Scholar
  8. 8.
    A. S. Shahbang, J. J. Jacobs, J. Black, J. O. Galante and T. T. Glant, J. Arthroplasty 10 (1995) 498.Google Scholar
  9. 9.
    D. W. Murray and N. Rushton, J. Bone Joint Surg. 72B (1990) 988.Google Scholar
  10. 10.
    J. Quinn, C. J. Joyner, J. T. Triffitt and N. A. Athanasou, ibid. 74B (1992) 652.Google Scholar
  11. 11.
    R. Pandey, J. Quinn, C. Joyner, J. T. Triffitt and N. A. Athanasou, Ann. Rheum. Dis. 55 (1996) 388.Google Scholar
  12. 12.
    A. Sabokbar, R. Pandey, J. Quinn and N. A. Athanasou, Arch. Orthop. Trauma Surg. 184 (1998) 31.Google Scholar
  13. 13.
    A. Sabokbar, Y. Fujikawa, D. W. Murray and N. A. Athanasou, J. Bone Joint Surg. 79B (1997) 129.Google Scholar
  14. 14.
    Y. Fujikawa, J. M. W. Quinn, A. Sabokbar, J. O'D. Mcgee and N. A. Athanasou, Endocrinology 139 (1996) 4058.Google Scholar
  15. 15.
    A. Sabokbar, Y. Fujikawa, S. Neale, D. Murray and N. A. Athanasou, Ann. Rheum. Dis. 56 (1997) 414.Google Scholar
  16. 16.
    S. D. Neale, D. R. Haynes, D. W. Howie, D. W. Murray and N. A. Athanasou, J. Arthroplasty 15 (2000) 654.Google Scholar
  17. 17.
    I. Itonaga, A. Sabokbar, D. W. Murray and N. A. Athanasou, Ann. Rheum. Dis. 59 (2000) 26.Google Scholar
  18. 18.
    H. Gelb, H. R. Schumacher, J. Cuckler and D. G. Baker, J. Orthop. Res. 12 (1994) 83.Google Scholar
  19. 19.
    A. S. Shanbhang, J. J. Jacobs, J. Black, J. O. Galante and T. T. Glant, J. Biomed. Mater. Res. 28 (1994) 81.Google Scholar
  20. 20.
    W. J. Maloney, R. L. Smith, D. Hvene, T. P. Schmalzried and H. Rubash, J. Bone Joint Surg. 76A (1994) 1664.Google Scholar
  21. 21.
    A. S. Shanbhang, J. J. Jacobs, T. Glant, J. L. Gilbert, J. Black and J. O. Galante, ibid. 76B (1994) 60.Google Scholar
  22. 22.
    E. Ingham and J. Fisher, Proc. Inst. Mech. Eng. 214 (2000) 21.Google Scholar
  23. 23.
    T. Suda, N. Udagawa, I. Nakamura, C. Miyaura and N. Takahashi, Bone 17 (1995) 87S.Google Scholar
  24. 24.
    N. A. Athanasou, J. Bone Joint Surg. 78A (1996) 1096.Google Scholar
  25. 25.
    M. Krukowski, R. A. Shively, P. Osdoby and B. L. Eppley, Oral Maxillofac. Surg. 48 (1990) 468.Google Scholar
  26. 26.
    R. D. Galiano, R. W. Jyung, M. Krukowski and T. A. Mustoe, Ann. Plast. Surg. 36 (1996) 608.Google Scholar
  27. 27.
    W. Brodner, P. Bitzan, V. Meisinger, A. Kaider, F. Gottsauner-Wolf and R. Kotz, J. Bone Joint Surg. Br. 79 (1997) 316.Google Scholar
  28. 28.
    H. G. Willert, G. H. H. Buchhorn, D. Göbel, G. Köster, S. Schaffner, R. Schnk and M. Semlitsch, Clin. Orthop. 329S (1996) S160.Google Scholar
  29. 29.
    C. Minkin, Calcif. Tissue Int. 34 (1982) 285.Google Scholar
  30. 30.
    J. M. Austyn and S. Gordon, Eur. J. Immunol. 11 (1981) 805.Google Scholar
  31. 31.
    H. C. Amstutz, P. Campbell, N. Kossovsky and I. C. Clarke, Clin. Orthop. Rel. Res. 276 (1992) 7.Google Scholar
  32. 32.
    M. Jasty, C. Bragdon, W. Jiranek, H. Chandler, W. Maloney and W. H. Harris, ibid. 308 (1994) 111.Google Scholar
  33. 33.
    J. H. Herman, W. G. Sowder, D. Anderson, A. M. Appel and C. N. Hopson, J. Bone Joint Surg. 71A (1989) 1530.Google Scholar
  34. 34.
    S. M. Horowitz, T. L. Gautsch, C. G. Frondoza and L. Riley, J. Orthop. Res. 9 (1991) 406.Google Scholar
  35. 35.
    T. R. Green, J. Fisher, M. Stone, B. M. Wroblewski and E. Ingham, Biomaterials 19 (1998) 2297.Google Scholar
  36. 36.
    T. T. Glant and J. Jacobs, J. Orthop. Res. 12 (1994) 720.Google Scholar
  37. 37.
    J. B. Matthews, T. R. Green, M. H. Stone, B. M. Wroblewski, J. Fisher and E. Ingham, Biomaterials 21 (2000) 2033.Google Scholar
  38. 38.
    A. A. Ragab, R. Van De Motter, S. A. Lavish, V. M. Goldberg, J. T. Ninomiya, C. R. Carlin and E. M. Greenfield, J. Orthop. Res. 17 (1999) 803.Google Scholar
  39. 39.
    T. R. Green, J. Fisher, J. B. Matthews, M. Stone and E. Ingham, J. Biomed. Mater. Res. 53 (2000) 490.Google Scholar
  40. 40.
    I. Voronov, J. P. Santerre, A. Hinek, J. W. Callahan, J. Sandhu and E. L. Boynton, ibid. 39 (1998) 40.Google Scholar
  41. 41.
    Y. Nakashima, D. H. Sun, M. C. Trindale, W. J. Maloney, S. B. Goodman, D. J. Schurman and R. L. Smith, J. Bone Joint Surg. 81A (1999) 603.Google Scholar
  42. 42.
    E. M. Schwarz, A. P. Lu, J. J. Goater, E. B. Benz, G. Kollias, R. N. Rosier, J. E. Puzas and R. J. O'Keefe, J. Orthop. Res. 18 (2000) 472.Google Scholar
  43. 43.
    W. Wang, D. J. Ferguson, J. M. Quinn, A. H. Simpson and N. A. Athanasou, J. Bone Joint Surg. 79B (1997) 849.Google Scholar
  44. 44.
    W. Wang, D. J. Ferguson, J. M. Quinn, A. H. Simpson and N. A. Athanasou, J. Pathol. 182 (1997) 92.Google Scholar

Copyright information

© Kluwer Academic Publishers 2003

Authors and Affiliations

  1. 1.Nuffield Department of Orthopaedic SurgeryUniversity of Oxford, Nuffield Orthopaedic Centre, HeadingtonOxfordUK

Personalised recommendations