Biochemistry (Moscow)

, Volume 68, Issue 7, pp 776–782 | Cite as

Role of Calcium Ions in Rapid Effects of L-Thyroxine on Phosphoinositide Metabolism in Rat Liver Cells

  • O. A. Krasilnikova
  • N. S. Kavok
  • N. A. Babenko
Article

Abstract

The role of calcium ions in the L-thyroxine-induced initiation of hydrolysis of phosphatidylinositol 4,5-bisphosphate (PtdInsP2) and also the course of releasing individual fractions of inositol phosphates and diacylglycerides (DAG) were studied in liver cells during early stages of the hormone effect. L-Thyroxine stimulated a rapid hydrolysis in hepatocytes of PtdInsP2 labeled with [14C]linoleic acid and [3H]inositol mediated by phosphoinositide-specific phospholipase C. This was associated with accumulation of [14C]DAG, total inositol phosphates, [3H]inositol 1,4,5-trisphosphate (Ins1,4,5P3) and [3H]inositol 1,4-bisphosphate (Ins1,4P2). Elimination of calcium ions from the incubation medium of hepatocytes did not abolish the effect of thyroxine on the accumulation of [14C]DAG and total [3H]inositol phosphates. Preincubation of liver cells with TMB-8 increased the stimulatory effect of L-thyroxine on the accumulation of [14C]DAG. During the incubation of hepatocytes in the presence of the hormone the content of 14C-labeled fatty acids did not change. The L-thyroxineinduced accumulation of [3H]Ins1,4,5P3 and [3H]Ins1,4P2 did not depend on the presence of calcium ions in the incubation medium of the cells.

thyroxine hepatocytes phospholipase C phosphatidylinositol 4,5-bisphosphate diacylglycerol inositol 1,4,5-trisphosphate calcium 

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Copyright information

© MAIK “Nauka/Interperiodica” 2003

Authors and Affiliations

  • O. A. Krasilnikova
    • 1
  • N. S. Kavok
    • 1
  • N. A. Babenko
    • 1
  1. 1.Institute of BiologyKharkov National University, pl. Svobody 4KharkovUkraine

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