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Plant Growth Regulation

, Volume 40, Issue 3, pp 249–256 | Cite as

Biochemical and photochemical changes in response to triacontanol in rice (Oryza sativa L.)

  • Xinping Chen
  • Hongyu Yuan
  • Rongzhi Chen
  • Lili Zhu
  • Guangcun He
Article

Abstract

Triacontanol (TRIA) increased the contents of total chlorophyll (Chl), Chl a and Chl b by 25.1%, 26.1% and 22.4% respectively 4 h after treatment in rice seedlings. The minimal fluorescence (F0), the maximal fluorescence (Fm) and Fv/Fm were also higher in TRIA-treated plants. In actinic light, other Chl fluorescence parameters were measured at different photon flux densities (PFD) to construct light response curves of the quantum yield of PSII electron transport (ϕPSII), light response curves of photochemical quenching (qp), and light response curves of non-photochemical quenching (qN), respectively. The ϕPSII and qp declined with the increasing PFD with a higher level present in TRIA-treated plants. The qN increased with the increasing PFD with a lower level present in TRIA-treated plants. Two-dimensional gel electrophoresis indicated a protein expression difference between TRIA-treated materials and the controls at the total-soluble-protein level. Rubisco was ∼30% higher in TRIA-treated plants than in controls. The quantity of other proteins was unchanged in response to TRIA. These data provide biochemical and photochemical evidence for the effects of TRIA on photosynthesis.

Chlorophyll fluorescence Photosystem Rubisco Triacontanol Two-dimensional gel electrophoresis 

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Copyright information

© Kluwer Academic Publishers 2003

Authors and Affiliations

  • Xinping Chen
    • 1
    • 2
  • Hongyu Yuan
    • 1
  • Rongzhi Chen
    • 1
  • Lili Zhu
    • 1
  • Guangcun He
    • 1
  1. 1.Key Laboratory of the Ministry of Education for Plant Developmental BiologyWuhan UniversityWuhanChina
  2. 2.College of Life SciencesWuhan UniversityWuhanChina

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