Biotechnology Letters

, Volume 25, Issue 17, pp 1473–1477 | Cite as

Immobilized laccase for decolourization of Reactive Black 5 dyeing effluent

  • Andrea Zille
  • Tzanko Tzanov
  • Georg M. Gübitz
  • Artur Cavaco-Paulo


Reactive Black 5 industrial dyeing effluent was decolourized by free and immobilized laccase. The stability of the enzyme (194 h free and 79 h immobilized) depended on the dyeing liquor composition and the chemical structure of the dye. In the decolourization experiments with immobilized laccase, two phenomenons were observed – decolourization due to adsorption on the support (79%) and dye degradation due to the enzyme action (4%). Dyeing in the enzymatically recycled effluent provided consistency of the colour with both bright and dark dyes.

decolourization laccase recycling of dyeing effluents stability 


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Copyright information

© Kluwer Academic Publishers 2003

Authors and Affiliations

  • Andrea Zille
    • 1
  • Tzanko Tzanov
    • 1
  • Georg M. Gübitz
    • 2
  • Artur Cavaco-Paulo
    • 1
  1. 1.Department of Textile EngineeringUniversity of MinhoGuimarãesPortugal
  2. 2.Department of Environmental BiotechnologyGraz University of TechnologyGrazAustria

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