Plant Molecular Biology

, Volume 52, Issue 4, pp 873–891

Temporal progression of gene expression responses to salt shock in maize roots

  • Hong Wang
  • Saori Miyazaki
  • Kiyoshi Kawai
  • Michael Deyholos
  • David W. Galbraith
  • Hans J. Bohnert
Article

DOI: 10.1023/A:1025029026375

Cite this article as:
Wang, H., Miyazaki, S., Kawai, K. et al. Plant Mol Biol (2003) 52: 873. doi:10.1023/A:1025029026375

Abstract

Using a cDNA microarray containing 7943 ESTs, the behavior of the maize root transcriptome has been monitored in a time course for 72 h after imposition of salinity stress (150 mM NaCl). Under these conditions, root sodium amounts increased faster than in leaves, and root potassium decreased significantly. Although the overall free amino acid concentration was not affected, amino acid composition was changed with proline and asparagine increasing. Microarray analysis identified 916 ESTs representing genes whose steady-state RNA levels were significantly altered at various time points, corresponding to 11% of the ESTs printed. The response of the transcriptome to sub-lethal salt stress was rapid and transient, leading to a burst of changes at the three-hour time point. The salt-regulated ESTs represented 472 tentatively unique genes (TUGs), which, based on functional category analysis, are involved in a broad range of cellular and biochemical activities, prominent amongst which were transport and signal transduction pathways. Clustering of regulated transcripts based on the timing and duration of changes suggests a structured succession of induction and repression for salt responsive genes in multiple signal and response cascades. Within this framework, 16 signaling molecules, including six protein kinases, two protein phosphatases and eight transcription factors, were regulated with distinct expression patterns by high salinity.

gene expression profile microarray salt stress time course Zea mays 

Copyright information

© Kluwer Academic Publishers 2003

Authors and Affiliations

  • Hong Wang
    • 1
  • Saori Miyazaki
    • 2
    • 3
  • Kiyoshi Kawai
    • 2
  • Michael Deyholos
    • 4
    • 5
  • David W. Galbraith
    • 1
  • Hans J. Bohnert
    • 2
    • 3
  1. 1.Department of Plant SciencesUniversity of ArizonaTucsonUSA
  2. 2.Department of Biochemistry and Molecular BiophysicsUniversity of ArizonaTucsonUSA
  3. 3.Department of Plant Biology and Department of Crop SciencesUniversity of IllinoisUrbanaUSA
  4. 4.Department of Plant SciencesMolecular BiophysicsCanada
  5. 5.Department of Biological SciencesUniversity of AlbertaEdmontonCanada

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