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Biotechnology Letters

, Volume 25, Issue 15, pp 1251–1254 | Cite as

Components in the inoculum determine the kinetics of Azotobacter vinelandii cultures and the molecular weight of its alginate

  • Mauricio A. Trujillo-Roldán
  • Carlos Peña
  • Enrique Galindo
Article

Abstract

In cultures of Azotobacter vinelandii inoculated using washed cells (avoiding exhausted broth components) alginates of a higher molecular weight (1200 kDa) than those obtained in cultures conventionally inoculated (350 kDa), were produced. Also, when comparing conventionally inoculated cultures with those inoculated with washed-cells, the alginate lyase activity was delayed and the final polymer concentration decreased from 4.8 to 3.5 g l−1. This suggests that components in the exhausted inoculum broth play important regulatory roles in alginate biosynthesis and needs to be taken into account when describing polymer biosynthesis.

alginate alginate lyase Azotobacter vinelandii 

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References

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Copyright information

© Kluwer Academic Publishers 2003

Authors and Affiliations

  • Mauricio A. Trujillo-Roldán
    • 1
  • Carlos Peña
    • 1
  • Enrique Galindo
    • 1
  1. 1.Departamento de Ingeniería Celular y BiocatálisisInstituto de Biotecnología, Universidad Nacional Autónoma de MéxicoMorelosMéxico

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