Biotechnology Letters

, Volume 25, Issue 15, pp 1281–1285

Large-scale production and purification of recombinant Galanthus nivalis agglutinin (GNA) expressed in the methylotrophic yeast Pichia pastoris

  • Philippe Baumgartner
  • Karen Harper
  • Romaan J.M. Raemaekers
  • Alain Durieux
  • Angharad M.R. Gatehouse
  • Howard V. Davies
  • Mark A. Taylor
Article

Abstract

The gene coding for agglutinin from Galanthus nivalis (GNA) was expressed in, and secreted by, the methylotrophic yeast, Pichia pastoris. Transformants of P. pastoris were selected and a process to produce and purify gram quantities of recombinant GNA was developed. GNA was secreted at approximately 80 mg l−1 at the 200 l scale and was purified to 95% homogeneity using hydrophobic interaction chromatography. The recombinant protein was similar to the protein synthesised in plant with respect to structure and biological activity.

fed-batch culture Galanthus nivalis agglutinin large-scale purification lectin Pichia pastoris 

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Copyright information

© Kluwer Academic Publishers 2003

Authors and Affiliations

  • Philippe Baumgartner
    • 1
  • Karen Harper
    • 1
  • Romaan J.M. Raemaekers
    • 2
  • Alain Durieux
    • 3
  • Angharad M.R. Gatehouse
    • 2
  • Howard V. Davies
    • 1
  • Mark A. Taylor
    • 1
  1. 1.Quality, Health & Nutrition GroupScottish Crop Research InstituteDundeeScotland
  2. 2.Department Agricultural and Environmental Sciences, King George VI BuildingUniversity of NewcastleNewcastle upon TyneUK
  3. 3.Unité de BiotechnologieInstitut MeuriceBrusselsBelgium

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