Neurochemical Research

, Volume 28, Issue 9, pp 1299–1306 | Cite as

Angiotensin II Causes Calcium Entry into Bovine Adrenal Chromaffin Cells via Pathway(s) Activated by Depletion of Intracellular Calcium Stores

  • David A. Powis
  • Mariann Zerbes
  • Lynn M. Herd
  • Peter R. Dunkley


The characteristics and properties of the increase in cytosolic [Ca2+] that occurs in bovine adrenal medullary chromaffin cells on exposure to angiotensin II have been investigated. In fura-2 loaded cells exposure to a maximally effective concentration of angiotensin II (100 nM) caused a rapid, but transient increase in cytosolic [Ca2+] followed by a lower plateau that was sustained as long as external Ca2+ was present. In the absence of external Ca2+ only the initial brief transient was observed. In cells previously treated with thapsigargin in Ca2+-free medium to deplete the internal Ca2+ stores, angiotensin II caused no increase in cytosolic [Ca2+] when external Ca2+ was absent. Reintroduction of external Ca2+ to thapsigargin-treated, store-depleted cells caused a sustained increase in cytosolic [Ca2+] that was not further increased upon exposure to angiotensin II. Analysis of the data suggests that in bovine chromaffin cells angiotensin II causes Ca2+ entry via a pathway(s) activated as a consequence of internal store mobilization, and entry through this pathway(s) forms the majority of the sustained Ca2+ influx evoked by angiotensin II.

Bovine chromaffin cells angiotensin II Ca2+ entry pathways intracellular Ca2+ stores 


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Copyright information

© Plenum Publishing Corporation 2003

Authors and Affiliations

  • David A. Powis
    • 1
  • Mariann Zerbes
    • 1
  • Lynn M. Herd
    • 1
  • Peter R. Dunkley
    • 1
  1. 1.School of Biomedical Sciences, Faculty of HealthThe University of NewcastleNew South WalesAustralia

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