Enhanced HAPEX topography: Comparison of osteoblast response to established cement

  • M. J. DalbyEmail author
  • W. Bonfield
  • L. Di Silvio


The use of poly(methylmethacrylate) PMMA cement by Charnley in the 1960s revolutionized orthopaedic medicine. Since this time, however, little has changed. The development of bioactive composites, such as HAPEX™ (a composite of 40% vol hydroxyapatite (HA) in a polyethylene matrix) have potential in orthopaedic applications. The composite has been shown to allow direct bone bonding in vivo, and in vitro studies have shown preferential attachment to HA exposed on the composite surface. In vitro study has also shown that altering the topography HAPEX™ can enhance osteoblast response. This study uses microscopical investigation of osteoblast cytoskeleton, and biochemical measurement of proliferation (by thymidine incorporation) and phenotype (by alkaline phosphatase activity) to compare primary human osteoblast (HOB) activity on HAPEX™ and PMMA cement. The study shows large increases in HOB response to the new generation material compared to PMMA, the current implant standard.


PMMA Hydroxyapatite Thymidine Methylmethacrylate Alkaline Phosphatase Activity 
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Copyright information

© Kluwer Academic Publishers 2003

Authors and Affiliations

  1. 1.Institute of Orthopaedics, IRC in Biomedical Materials, Royal National Orthopaedic HospitalMiddlesexUK
  2. 2.IRC in Biomedical Materials, Queen Mary and Westfield CollegeLondonUK

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