Molecular Biology Reports

, Volume 30, Issue 3, pp 177–183

Molecular cloning and characterization of a novel mannose-binding lectin gene from Amorphophallus konjac

  • Jiong Fei
  • Zhihua Liao
  • Yourong Chai
  • Yongzhen Pang
  • Jianhong Yao
  • Xiaofen Sun
  • Kexuan Tang
Article

DOI: 10.1023/A:1024956525396

Cite this article as:
Fei, J., Liao, Z., Chai, Y. et al. Mol Biol Rep (2003) 30: 177. doi:10.1023/A:1024956525396

Abstract

A new lectin gene was cloned from Amorphophallus konjac. The full-length cDNA of Amorphophallus konjac agglutinin (aka) was 736 bp and contained a 474 bp open reading frame encoding a 158 amino acid protein. Homology analysis revealed that the lectin from this Araceae species belonged to the superfamily of monocot mannose-binding proteins. Molecular modeling of AKA indicated that the three-dimensional structure of AKA strongly resembles that of the snowdrop lectin. Southern blot analysis of the genomic DNA revealed that aka belonged to a low-copy gene family. Northern blot analysis demonstrated that aka expression was tissue-specific with the strongest expression being found in root.

Amophophallus konjac cDNA mannose-binding lectin molecular cloning 

Copyright information

© Kluwer Academic Publishers 2003

Authors and Affiliations

  • Jiong Fei
    • 1
    • 1
  • Zhihua Liao
    • 1
  • Yourong Chai
    • 1
  • Yongzhen Pang
    • 1
  • Jianhong Yao
    • 1
  • Xiaofen Sun
    • 1
    • 1
  • Kexuan Tang
    • 1
    • 1
  1. 1.Fudan-SJTU-Nottingham Plant Biotechnology R&D Center, Plant Biotechnology Center, School of Agriculture and BiologyShanghai Jiaotong UniversityShanghaiChina

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