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Journal of Biomolecular NMR

, Volume 27, Issue 2, pp 133–142 | Cite as

Rotational diffusion tensor of nucleic acids from 13C NMR relaxation

  • Jerome Boisbouvier
  • Zhengrong Wu
  • Akira Ono
  • Masatsune Kainosho
  • Ad Bax
Article

Abstract

Rotational diffusion properties have been derived for the DNA dodecamer d(CGCGAATTCGCG)2 from 13C R and R1 measurements on the C1′, C3′, and C4′ carbons in samples uniformly enriched in 13C. The narrow range of C-H bond vector orientations relative to the DNA axis make the analysis particularly sensitive to small structural deviations. As a result, the R/R1 ratios are found to fit poorly to the crystal structures of this dodecamer, but well to a recent solution NMR structure, determined in liquid crystalline media, even though globally the structures are quite similar. A fit of the R/R1 ratios to the solution structure is optimal for an axially symmetric rotational diffusion model, with a diffusion anisotropy, D||/D, of 2.1±0.4, and an overall rotational correlation time, (2D||+4D)−1, of 3.35 ns at 35 °C in D2O, in excellent agreement with values obtained from hydrodynamic modeling.

anisotropy 13C relaxation Dickerson dodecamer diffusion anisotropy nucleic acids rotational diffusion 

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Copyright information

© Kluwer Academic Publishers 2003

Authors and Affiliations

  • Jerome Boisbouvier
    • 1
  • Zhengrong Wu
    • 1
  • Akira Ono
    • 2
  • Masatsune Kainosho
    • 2
  • Ad Bax
    • 1
  1. 1.Laboratory of Chemical PhysicsNational Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of HealthBethesdaU.S.A
  2. 2.CREST and Graduate School of ScienceTokyo Metropolitan UniversityHachioji, TokyoJapan

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