Engineered articular cartilage: influence of the scaffold on cell phenotype and proliferation

  • Antonio GiganteEmail author
  • Claudia Bevilacqua
  • Massimo Cappella
  • Sandra Manzotti
  • Francesco Greco


Articular cartilage defects do not heal. Biodegradable scaffolds have been studied for cartilage engineering in order to implant autologous chondrocytes and help cartilage repair. We tested some new collagen matrices differing in collagen type, origin, structure and methods of extraction and purification, and compared the behavior of human chondrocytes cultured on them. Human chondrocytes were grown for three weeks on four different equine type I collagen matrices, one type I, III porcine collagen matrix and one porcine type II collagen matrix. After 21 days, samples were subjected to histochemical, immunohistochemical and histomorphometric analysis to study phenotype expression and cell adhesion. At 7, 14 and 21 days cell proliferation was studied by incorporation of [3H]-thymidine. Our data evidence that the collagen type influences cell morphology, adhesion and growth; indeed, cellularity and rate of proliferation were significantly higher and cells were rounder on the collagen II matrix than on either of the collagen I matrices. Among the collagen I matrices, we observed a great variability in terms of cell adhesion and proliferation. The present study allowed us to identify one type I collagen matrix and one type II collagen matrix that could be usefully employed as a scaffold for chondrocyte transplantation.


Cell Adhesion Thymidine Articular Cartilage Collagen Type Cartilage Defect 
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  1. 1.
    J. Buckwalter, L. Rosemberg and E. HunzikerArticular Cartilage: Composition, Structure, Response to Injury, and Methods of Facilitating Repair (Raven Press, New York, 1990).Google Scholar
  2. 2.
    J. E. Browne and T. P. Branch, J. Am. Acad. Orthop. Surg. 8 (2000) 180.Google Scholar
  3. 3.
    L. Peterson, T. Minas, M. Brittberg, A. Nilsson, E. Sjogren-Jansson and A. Lindahl, Clin. Ortop. 374 (2000) 212.Google Scholar
  4. 4.
    K. Messner and J. Gillquist, Acta. Orthop. Scand. 67 (1996) 523.Google Scholar
  5. 5.
    A. Gigante, C. Bevilacqua, C. Zara, M. Travasi and C. Chillemi, Knee Surg. Sports Traumatol. Arthrosc. 9 (2001) 254.Google Scholar
  6. 6.
    S. Nehrer, H. A. Breinan, A. Ramappa, G. Young, S. Shortkroff, L. K. Louie, C. B. Sledge, I. V. Yannas and M. Spector, Biomaterials 18 (1997) 769.Google Scholar
  7. 7.
    C. Perka, O. Schultz, K. Lindenhayn, R. S. Spitzer, M. Muschik, M. Sittinger and G. R. Burmester, Clin. Exp. Rheumatol. 18 (2000) 13.Google Scholar
  8. 8.
    G. A. Ameer, T. A. Mahmood and R. Langer, J. Orthop. Res. 20 (2002) 16.Google Scholar
  9. 9.
    B. Grigolo, L. Roseti, M. Fiorini, M. Fini, G. Giavaresi, N. Nicoli Aldini, R. Giardino and A. Facchini, Biomaterials 22 (2001) 2417.Google Scholar
  10. 10.
    L. E. Freed, D. A. Grande, Z. Lingbin, J. Emmanual, J. C. Marquis and R. Langer, J. Biomed. Mater. Res. 28 (1994) 891.Google Scholar
  11. 11.
    S. Nehrer, H. A. Breinan, A. Ramappa, S. Shortkroff, G. Young, T. Minas, C. B. Sledge, I. V. Yannas and M. Spector, ibid. 38 (1997) 95.Google Scholar
  12. 12.
    S. Kawamura, S. Wakitani, T. Kimura, A. Maeda, A. I. Caplan, K. Shino and T. Ochi, Acta. Orthop. Scand. 69 (1998) 56.Google Scholar
  13. 13.
    J. L. C. Van Susante, J. Pieper, P. Buma, T. H. Van Kuppevelt, H. Van Beuningen, P. M. Van Der Kraan, J. H. Veerkamp, W. B. Van Den Berg and R. P. H. Veth, Biomaterials 22 (2001) 2359.Google Scholar
  14. 14.
    C. L. Tsai, S. H. Hsu and W. L. Cheng, Artif. Organs 26 (2002) 18.Google Scholar
  15. 15.
    C. Chillemi, C. Bevilacqua and A. Gigante, Eur. J. Histochem. 43 (1999) 227.Google Scholar
  16. 16.
    C. W. Archer, J. Mcdowell, M. T. Bayliss, M. D. Stephens and G. Bentley, J. Cell. Sci. 97 (1990) 361.Google Scholar
  17. 17.
    P. D. Benya and J. D. Shaffer, Cell 30 (1982) 215.Google Scholar
  18. 18.
    R. D. Coutts, R. L. Sah and D. Amiel, Instr. Course Lect. 46 (1997) 487.Google Scholar

Copyright information

© Kluwer Academic Publishers 2003

Authors and Affiliations

  • Antonio Gigante
    • 1
    Email author
  • Claudia Bevilacqua
    • 1
  • Massimo Cappella
    • 1
  • Sandra Manzotti
    • 1
  • Francesco Greco
    • 1
  1. 1.Department of OrthopaedicsUniversity of AnconaAnconaItaly

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