Biodegradation

, Volume 14, Issue 4, pp 241–247 | Cite as

Growth-Substrate Dependent Dechlorination of 1,2-Dichloroethane by a Homoacetogenic Bacterium

  • Stefaan De Wildeman
  • Anke Neumann
  • Gabriele Diekert
  • Willy Verstraete
Article

Abstract

A rod shaped, gram positive, non sporulating Acetobacterium strain was isolated that dechlorinated 1,2-dichloroethane (1,2-DCA) to ethene at a dechlorination rate of up to 2 nmol Cl- min-1 mg-1 of protein in the exponential growth phase with formate (40 mM) as the substrate. Although with other growth substrates such as pyruvate, lactate, H2/CO2, and ethanol higher biomass productions were obtained,the dechlorination rate with these substrates was more than 10-fold lower compared with formate growing cells. Neither cell extracts nor autoclaved cells of the isolatedAcetobacterium strain mediated the dechlorination of 1,2-DCA at significant rates. The addition of 1,2-DCA to the media did not result in increased cell production. No significant differences in corrinoid concentrations could be measured in cells growing on several growth-substrates. However, these measurements indicated that differences in corrinoid structure might cause the different dechlorination activity. The Acetobacterium sp. strain gradually lost its dechlorination ability during about 10 transfers in pure culture, probably due to undefined nutritional requirements. 16S rDNA analysis of the isolate revealed a 99.7% similarity with Acetobacterium wieringae. However, the type strains of A. wieringae and A. woodii did not dechlorinate 1,2-DCA.

Acetobacterium sp. 1,2-dichloroethane homo-acetogenic reductive dechlorination 

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Copyright information

© Kluwer Academic Publishers 2003

Authors and Affiliations

  • Stefaan De Wildeman
    • 1
  • Anke Neumann
    • 2
  • Gabriele Diekert
    • 2
  • Willy Verstraete
    • 1
  1. 1.Laboratory of Microbial Ecology and Technology, Faculty of Agricultural and Applied Biological SciencesGhent UniversityGhentBelgium
  2. 2.Institute for Microbiology, Department of Applied and Ecological MicrobiologyFriedrich-Schiller University JenaJenaGermany

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