Plant Cell, Tissue and Organ Culture

, Volume 75, Issue 1, pp 49–55 | Cite as

Superoxide dismutase activity in callus from the C3-CAM intermediate plant Mesembryanthemum crystallinum

  • Ireneusz Ślesak
  • Marta Libik
  • Zbigniew Miszalski


In light-grown callus obtained from M. crystallinum hypocotyls, three classes of superoxide dismutase (SOD): Mn-, Fe- and Cu/ZnSOD were identified. Callus cultured on a medium containing 0.4 M NaCl showed an increase in FeSOD activity on day 4 of the experiment. In contrast, Cu/ZnSOD activity was higher over 16 days of the experiment. Salinity stress induces oxidative stress mainly for the cytosolic SOD form (Cu/ZnSOD). After 16 days of callus culture on salt-containing medium, diurnal malate oscillations, and an increase in NADP-malic enzyme activity were noticed. These results strongly suggest that C3-CAM transition can also be expressed at the cellular level. Therefore, callus tissue could be a useful model, similar to a whole plant, for investigation of mechanisms of stress responses in M. crystallinum.

callus CAM (Crassulacean acid metabolism) NADP-malic enzyme oxidative stress 


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Copyright information

© Kluwer Academic Publishers 2003

Authors and Affiliations

  • Ireneusz Ślesak
    • 1
  • Marta Libik
    • 1
  • Zbigniew Miszalski
    • 1
  1. 1.Department of Plant PhysiologyPolish Academy of SciencesKrakówPoland

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