Euphytica

, Volume 132, Issue 2, pp 185–190 | Cite as

Molecular cytogenetics in hop (Humulus lupulus L.) and identification of sex chromosomes by DAPI-banding

  • G.I. Karlov
  • T.V. Danilova
  • C. Horlemann
  • G. Weber
Article

Abstract

Hop (Humulus lupulus L.) waskaryotyped by Fluorescence In SituHybridization (FISH) with probes 18S-25Sand 5S rDNA as well as DAPI banding andmeasurements of lengths of chromosome.Irrespective of the sex of the plants theseprobes revealed one signal near thetelomere of chromosome 6 and two signalseach on chromosomes 2 and 5.The shortest chromosome in a metaphaseplate of a male plant was designated to bechromosome Y. It displayed neither signalswith FISH nor DAPI bands. A middle sizedmetacentric chromosome with no telomericbut with an interstitial DAPI band on theshort arm was designated as X. Allautosomes displayed telomeric DAPI-positivebands. For the first time all mitotic hopchromosomes including the sex chromosomeswere identified by methods of molecularcytogenetics.

fluorescence in situ hybridization hop Humulus lupulus (L.) karyotype sex chromosomes rDNA 

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Copyright information

© Kluwer Academic Publishers 2003

Authors and Affiliations

  • G.I. Karlov
    • 1
  • T.V. Danilova
    • 1
  • C. Horlemann
    • 2
  • G. Weber
    • 2
  1. 1.Department of BiotechnologyTimiryazev Agricultural AcademyMoscowRussia
  2. 2.Institute of Plant Breeding, Seed Science and Population GeneticsUniversity of Hohenheim, Department of Plant Breeding and BiotechnologyStuttgartGermany

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