Molecular cytogenetics in hop (Humulus lupulus L.) and identification of sex chromosomes by DAPI-banding
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- Karlov, G., Danilova, T., Horlemann, C. et al. Euphytica (2003) 132: 185. doi:10.1023/A:1024646818324
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Hop (Humulus lupulus L.) waskaryotyped by Fluorescence In SituHybridization (FISH) with probes 18S-25Sand 5S rDNA as well as DAPI banding andmeasurements of lengths of chromosome.Irrespective of the sex of the plants theseprobes revealed one signal near thetelomere of chromosome 6 and two signalseach on chromosomes 2 and 5.The shortest chromosome in a metaphaseplate of a male plant was designated to bechromosome Y. It displayed neither signalswith FISH nor DAPI bands. A middle sizedmetacentric chromosome with no telomericbut with an interstitial DAPI band on theshort arm was designated as X. Allautosomes displayed telomeric DAPI-positivebands. For the first time all mitotic hopchromosomes including the sex chromosomeswere identified by methods of molecularcytogenetics.