Euphytica

, Volume 132, Issue 1, pp 115–119 | Cite as

Genetic diversity among selected Argentinean garlic clones (Allium sativum L.) using AFLP (Amplified Fragment Length Polymorphism)

  • S. García Lampasona
  • L. Martínez
  • J.L. Burba
Article

Abstract

Genetic diversity of eight selected Argentinean garlic clones (Allium sativum L.) were investigated at the DNA level with the amplified fragment length polymorphism DNA (AFLP) procedure. A total of 405 unambiguous bands were identified by six primer combinations of EcoR I +3 and Mse I +3, of those, 398 showed a clear polymorphism, representing 98% of the total bands. A presence/absence matrix was constructed with the polymorphic bands, and a dendrogram was obtained from it with the UPGMA method. The accessions showed different levels of similarity ranging between 0.24 and 0.97, using the coefficient of Jaccard. The dendrogram showed six arbitrary groups. Accessions typically considered as different clones show similarities between 0.97 and 0.495. The garlic clones were clustered according to the physiological group and bulb color. We could detect an association between AFLP and the geographical origin of the clones. The potential use of AFLP could allow not only the differentiation among species, but also between botanical varieties and well-defined ecotype groups. This is the first report of the use of AFLP to characterize Argentinean garlic clones.

AFLP Allium sativum L. fingerprinting garlic molecular markers 

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Copyright information

© Kluwer Academic Publishers 2003

Authors and Affiliations

  • S. García Lampasona
    • 1
  • L. Martínez
    • 2
  • J.L. Burba
    • 3
  1. 1.EEA La Consulta INTA & Laboratorio de Biología Molecular, Facultad de Ciencias Agrarias, UNCuyoChacras de Coria, MendozaArgentina
  2. 2.Facultad de Ciencias Agrarias, UNCuyoLaboratorio de Biología MolecularArgentina
  3. 3.EEA La Consulta INTA, CC 8La Consulta, Mendoza

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