Methods in Cell Science

, Volume 24, Issue 4, pp 165–168 | Cite as

A simple flow-through micro-chamber for handling fragile, small tissue explants and single non-adherent cells

  • Peter Hausen
  • Metta Riebesell


The flow-through chambers described are easily assembled in the laboratory with no need for sophisticated or expensive equipment. They allow for treating micro-explants or single non-adhesive cells with different solutes for defined times by easy and quick changes of the medium in the chambers. The specimen can be fixed, immunostained and observed microscopically within the chamber. During these procedures the cells are not exposed to undue mechanical stress and fragile intercellular structures are well preserved.

Flow-through chamber Immunostaining Micro-explants Single cells 


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  1. 1.
    Angres B, Müller AHJ, Kellermann J, Hausen P. Differential expression of two cadherins in Xenopus laevis. Development 1991; 11: 829-844.Google Scholar
  2. 2.
    Barth LC, Jaeger-Barth L. Differentiation of cells of the Rana pipiens gastrula in unconditioned medium. J Embryol Exptl Morph. 1956; 7: 210-222.Google Scholar
  3. 3.
    Reintsch WE, Hausen P. Dorsoventral differences in cell-cell interactions modulate the motile behaviour in cells from the Xenopus embryo. Dev Biol 2001; 240: 387-403.Google Scholar
  4. 4.
    Schneider S, Herrenknecht K, Butz S, Kemler R, Hausen P. Catenins in Xenopus embryogenesis and their relation to the cadherin mediated cell-cell adhesion system. Development 1993; 118: 629-640.Google Scholar
  5. 5.
    Wedlich D, Müller AHJ, Kühl M, Finnemann S, Schneider S, van der Poel SZ, Hausen P. Xenopus cadherins: The maternal pool comprises distinguishable members of the family. Mech Dev 1994; 47: 213-223.Google Scholar

Copyright information

© Kluwer Academic Publishers 2002

Authors and Affiliations

  • Peter Hausen
    • 1
  • Metta Riebesell
    • 1
  1. 1.Max-Planck-Intitut für EntwicklungsbiologieSpemannstrGermany

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