Genetic Resources and Crop Evolution

, Volume 50, Issue 6, pp 611–614 | Cite as

Inter-simple sequence repeat (ISSR) and RAPD variation among wild barley (Hordeum. vulgare subsp. spontaneum) populations from west Turkey

  • Bahattin Tanyolac


Inter-Simple Sequence Repeat (ISSR) and Randomly Amplified Polymorphic DNA (RAPD) markers were used to analyze genetic distance among H. vulgare subsp. spontaneum populations from west Turkey. Fifty-five RAPD and 10 ISSR primers were used to detect variation among sample. A total of 55 polymorphic loci were found using 65 primers. Two distinct cluster groups were clearly established among populations. The minimum variation was detected between Pinarbasi and Bornova (GD = 0.192) populations and the maximum was found between Icmeler and Aydin populations (GD = 0.926). As two dominant markers, RAPD and ISSRs are effective and promising marker systems for detecting genetic variation.

Genetic variation H. vulgare subsp. spontaneum ISSR and RAPD markers 


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Copyright information

© Kluwer Academic Publishers 2003

Authors and Affiliations

  • Bahattin Tanyolac
    • 1
  1. 1.Department of BioengineeringEge University, BornovaIzmirTurkey

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