Journal of Chemical Ecology

, Volume 29, Issue 6, pp 1489–1499 | Cite as

Antiaphrodisiacs in Pierid Butterflies: A Theme with Variation!

  • Johan Andersson
  • Anna-Karin Borg-Karlson
  • Christer Wiklund


Male Pieris napi butterflies previously have been shown to synthesize and transfer an antiaphrodisiac, methyl salicylate (MeS), to females at mating. This substance curtails courtship and decreases the likelihood of female remating. Here, we show that similar systems occur in Pieris rapae and Pieris brassicae. In P. rapae, 13C-labeling studies showed that males utilize the amino acids phenylalanine and tryptophan as precursors to MeS and indole, respectively. These volatiles are transferred to females at mating and function as antiaphrodisiacs, as demonstrated by field tests entailing painting MeS, indole, or a mixture on the abdomens of virgin females and assessing their attractiveness to wild males. With P. brassicae, 13C-labeling studies showed that males use phenylalanine as a precursor to synthesize benzyl cyanide, which was demonstrated to function as an antiaphrodisiac by field tests similar to those for P. rapae. This communication system exhibits both similarities and differences among the three species; in P. napi and P. rapae, males are fragrant but transfer a volatile antiaphrodisiac to females that is completely different from the male odor, whereas in P. brassicae the antiaphrodisiac transferred by male to female is identical with male odor.

Pieridae pheromone methyl salicylate benzyl cyanide phenyl acetonitrile indole phenylalanine tryptophan 


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  1. Andersson, J., Borg-Karlson, A. K., and Wiklund, C. 2000. Sexual cooperation and conflict in butterflies: A male-transferred anti-aphrodisiac reduces harassment of recently mated females. Proc. R. Soc. London B 267:1271.Google Scholar
  2. Bissondath, C. and Wiklund, C. 1997. Effect of male body size on sperm precedence in the polyandrous butterfly Pieris napi. Behav. Ecol. 5:518–523.Google Scholar
  3. Boggs, C. L. and Gilbert, L. E. 1979. Male contribution to egg production in butterflies: Evidence for transfer of nutrients at mating. Science 206:83–84.Google Scholar
  4. Borg-Karlson, A. K. and Mozuraitis, R. 1996. Solid phase micro extraction technique used for collecting semiochemicals: Identification of volatiles released by individual signalling Phyllonorycter sylvella moths. Z. Naturforsch. 51c:599–602.Google Scholar
  5. Burns, J. M. 1968. Mating frequency in natural populations of skippers and butterflies as determined by spermatophore counts. Proc. Natl. Acad. Sci. U.S.A. 61:852–859.Google Scholar
  6. Drummond, B. A. III. 1984. Multiple mating and sperm competition in the Lepidoptera, pp. 291-370, in R. L. Smith (ed.). Sperm Competition and the Evolution of Animal Mating Systems. Academic Press, London.Google Scholar
  7. Ehrlich, A. H. and Ehrlich, P. R. 1978. Reproductive strategies in the butterflies: I. Mating frequency, plugging, and egg number. J. Kansas Entomol. Soc. 51:666–697.Google Scholar
  8. Forsberg, J. and Wiklund, C. 1989. Mating in the afternoon: Time-saving in courtship and remating by females of a polyandrous butterfly. Pieris napi. Behav. Ecol. Sociobiol. 25:349–356.Google Scholar
  9. Gilbert, L. E. 1976. Postmating female odor in Heliconius butterflies: A male-contributed antiaphrodisiac? Science 193:419–420.Google Scholar
  10. Happ, G. M. 1969. Multiple sex pheromones of the mealworm beetle, Tenebrio molitor L. Nature 222:180–181.Google Scholar
  11. Kaitala, A. and Wiklund, C. 1994. Polyandrous females forage for matings. Behav. Ecol. Sociobiol. 35:385–388.Google Scholar
  12. Kaitala, A. and Wiklund C. 1995. Female mate choice and mating costs in the polyandrous butterfly Pieris napi (Lepidoptera: Pieridae). J. Insect Behav. 8:355–363.Google Scholar
  13. Karlsson, B. 1998. Nuptial gifts, resource budgets, and reproductive output in a polyandrous butterfly. Ecology 79:2931–2940.Google Scholar
  14. Obara, Y. 1964. Mating behaviour of the cabbage white, Pieris rapae crucivora II: The “mate-refusal” posture of the female. Zool. Mag. 73:175–178.Google Scholar
  15. Parker, G. A. 1970. Sperm competition and its evolutionary consequences in the insects. Biol. Rev. 45:525–567.Google Scholar
  16. Silberglied, R. E. 1984. Visual communication and sexual selection among butterflies, pp. 207-223, in R. I. Vane-Wright and P. R. Ackery (Eds.). The Biology of Butterflies. Academic Press, London.Google Scholar
  17. Sväard, L. and Wiklund, C. 1989. Mass and production rate of ejaculates in relation to monandry/polyandry in butterflies. Behav. Ecol. Sociobiol. 24:395–402.Google Scholar
  18. Wedell, N. and Cook, P. A. 1998. Determinants of paternity in a butterfly. Proc. R. Soc. London B 265:625–630.Google Scholar
  19. Wiklund, C. and Forsberg, J. 1985. Courtship and male discrimination between virgin and mated females in the orange tip butterfly, Anthocharis cardamines. Anim. Behav. 34:328–332.Google Scholar
  20. Wiklund, C. and Forsberg, J. 1991. Sexual size dimorphism in relation to female polygamy and protandry in butterflies: A comparative study of Swedish Pieridae and Satyridae. Oikos 60:373–381.Google Scholar
  21. Wiklund, C., Kaitala, A., Lindfors, V., and Abenius, J. 1993. Polyandry and its effect on female reproduction in the green-veined white butterfly (Pieris napi L.). Behav. Ecol. Sociobiol. 33:25–33.Google Scholar
  22. Wiklund, C., Kaitala, A., and Wedell, N. 1998. Reproductive rates in relation to male and female parental expenditure in a polyandrous butterfly(Pieris napi L.) with large male nuptial gifts. Behav. Ecol. 9:20–25.Google Scholar

Copyright information

© Plenum Publishing Corporation 2003

Authors and Affiliations

  • Johan Andersson
    • 2
  • Anna-Karin Borg-Karlson
    • 1
  • Christer Wiklund
    • 2
  1. 1.Department of Chemistry, Organic Chemistry, Group of Ecological ChemistryRoyal Institute of Technology, SE-StockholmSweden
  2. 2.Department of ZoologyStockholm University, SE-StockholmSweden;

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