European Journal of Plant Pathology

, Volume 109, Issue 5, pp 445–458 | Cite as

Aerial Contamination of Sugarcane in Guadeloupe by Two Strains of Xanthomonas albilineans

  • J.H. Daugrois
  • V. Dumont
  • P. Champoiseau
  • L. Costet
  • R. Boisne-Noc
  • P. Rott

Abstract

Two sugarcane plots were set up in Guadeloupe with disease-free tissue cultured plants in a banana growing location distant from sugarcane fields. Thirteen weeks after planting sugarcane in the field, a Xanthomonas albilineans strain belonging to serotype 3 (strain XaS3) was detected in water sampled at sunrise on the leaves in the first plot. This strain randomly invaded the sugarcane canopy. Seven weeks later, a new strain belonging to serotype 1 (strain XaS1) appeared on leaves and populations of strain XaS1 progressively increased on the leaf surface, whereas populations of strain XaS3 progressively decreased. Leaf scald symptoms were first noted 26 weeks after sugarcane planting. However, only strain XaS1 was isolated from leaves and a few sugarcane stalks showing symptoms. Both strains also colonized the second field plot, which was studied at the end of the experiment to avoid human interference of aerial contamination of sugarcane. After inoculation of three sugarcane cultivars by the decapitation technique, strain XaS1 was as virulent or more virulent than five other strains of X. albilineans isolated from diseased sugarcane plants in Guadeloupe. Although strain XaS3 colonized a few stalks, it failed to produce any symptoms and was the least virulent strain. Leaf surface colonization by X. albilineans was reproduced in a greenhouse trial by spraying the pathogen on sugarcane foliage. After 8 weeks, the pathogen was isolated from disinfected leaf blades. Although the leaf scald pathogen is thought to be mainly transmitted by infected cuttings, aerial transmission of X. albilineans is also known to occur. These results indicate the importance of sugarcane phyllosphere colonization by virulent strains in the epidemiological cycle of leaf scald disease in Guadeloupe.

leaf scald epidemiology Saccharum spp. virulence epiphytic phyllosphere leaf surface 

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References

  1. Autrey LJC, Saumtally S, Dookun A, Sullivan S and Dhayan S (1995) Aerial transmission of the leaf scald pathogen, Xanthomonas albilineans. Proceedings International Society of Sugar Cane Technologists 21: 508-526Google Scholar
  2. Beattie GA and Lindow SE (1995) The secret life of foliar bacterial pathogens on leaves. Annual Review of Phytopathology 33: 145-172Google Scholar
  3. Beattie GA and Lindow SE (1999) Bacterial colonization of leaves: A spectrum of strategies. Phytopathology 89: 353-359Google Scholar
  4. Bernal RF and Berger RD (1996) The spread of epiphytic populations of Xanthomonas campestris pv. vesicatoria on pepper in the field. Journal of Phytopathology 114: 479-484Google Scholar
  5. Birch RG (2001) Xanthomonas albilineans and the antipathogenesis approach to disease control. Molecular Plant Pathology 2: 1-11Google Scholar
  6. Campbell CL and Madden LV (1990) Temporal analysis of epidemics. I: Description and comparison of disease progression curves. In: Introduction to Plant Disease Epidemiology (pp 161-202) John Wiley & Sons, New YorkGoogle Scholar
  7. Comstock JC (2001) Foliar symptoms of sugarcane leaf scald. Sugar Journal 64: 23-32Google Scholar
  8. Comstock JC and Irey MS (1992) Detection of the sugarcane leaf scald pathogen, Xanthomonas albilineans, using tissue blot immunoassay, ELISA, and isolation techniques. Plant Disease 76: 1033-1035Google Scholar
  9. Davis MJ, Rott P, Baudin P and Dean JL (1994) Evaluation of selective media and immunoassays for detection of Xanthomonas albilineans, causal agent of sugarcane leaf scald disease. Plant Disease 78: 78-82Google Scholar
  10. Davis MJ, Rott P, Warmuth CJ, Chatenet M and Baudin P (1997) Intraspecific genomic variation within Xanthomonas albilineans, the sugarcane leaf scald pathogen. Phytopathology 87: 316-324Google Scholar
  11. Dye DW (1980) Xanthomonas. In: Shaad NW (ed) Laboratory Guide for Identification of Plant Pathogenic Bacteria (pp 45-49) American Phytopathological Society, MinnesotaGoogle Scholar
  12. Egan BT and Sturgess OW (1980) Commercial control of leaf scald disease by thermotherapy and a clean seed program. Proceedings International Society of Sugar Cane Technologists 17: 1602-1606Google Scholar
  13. Feldmann P, Sapotille J, Grédoire P and Rott P (1994) Micropropagation of sugar cane. In: Teisson C (ed) In vitro Culture of Tropical Plants (pp 15-17) La Librairie du Cirad, Montpellier, FranceGoogle Scholar
  14. Flynn JL and Anderlini TA (1990) Disease incidence and yield performance of tissue culture generated seedcane over the crop cycle in Louisiana. Journal of American Society of Sugar Cane Technologists 10: 113Google Scholar
  15. Gagnevin L and Pruvost O (2001) Epidemiology and control of mango bacterial black spot. Plant Disease 85: 928-935Google Scholar
  16. Gottwald TR, Graham JH and Richie SM (1992a) Relationship of leaf surface-populations of strains of Xanthomonas campestris pv. citrumelo to development of citrus bacterial spot and persistence of disease symptoms. Phytopathology 82: 625-632Google Scholar
  17. Gottwald, TR, Richie SM and Campbell CL (1992b) LCOR2-Spatial correlation analysis software for the personal computer. Plant Disease 76: 213-215Google Scholar
  18. Hirano SS and Upper CD (1983) Ecology and epidemiology of foliar bacterial plant pathogens. Annual Review of Phytopathology 21: 243-269Google Scholar
  19. Hirano SS and Upper CD (1993) Dynamics, spread, and persistence of a single genotype of Pseudomonas syringae relative to those of its conspecifics on populations of snap bean leaflets. Applied Environmental Microbiology 59: 1082-1091Google Scholar
  20. Hoy JW and Grisham MP (1994) Sugarcane leaf scald distribution, symptomatology, and effect on yield in Louisiana. Plant Disease 78: 1086-1087Google Scholar
  21. Klett P and Rott P (1994) Inoculum sources for the spread of leaf scald disease of sugarcane caused by Xanthomonas albilineans in Guadeloupe. Journal of Phytopathology 142: 283-291Google Scholar
  22. Martin JP and Robinson PE (1961) Leaf scald. In: Martin JP, Abbott EV and Hughes CG (eds) Sugar-cane Disease of the World, Vol 1 (pp 79-107) Elsevier Publishing Co., AmsterdamGoogle Scholar
  23. Pan YB, Grisham MP, Burner DM and Wei Q (1999) Distribution of the leaf scald pathogen in infected sugarcane stalks. Journal of American Society of Sugar Cane Technologists 19: 8-15Google Scholar
  24. Ricaud C and Ryan CC (1989) Leaf scald. In: Ricaud C, Ryan BT, Gillaspie AG and Hughes CG (eds) Diseases of Sugarcane (pp 39-58) Elsevier Publishing Co., AmsterdamGoogle Scholar
  25. Rott P and Davis MJ (2000) Leaf scald. In: Rott P, Bailey RA, Comstock JC, Croft BJ and Saumtally AS (eds) A Guide to Sugarcane Disease (pp 38-44) La librairie du Cirad, Montpellier FranceGoogle Scholar
  26. Rott P, Chatenet M and Baudin P (1988) L'èchaudure des feuilles de la canne à sucre provoquèe par Xanthomonas albilineans (Ashby) Dowson. II. Diagnostic et spectres d'hôtes de l'agent pathogène en Afrique Tropicale. L'Agronomie Tropicale 43: 244-251Google Scholar
  27. Rott P, Abel M, Soupa D, Feldmann P and Letourmy P (1994a) Population dynamics of Xanthomonas albilineans in sugarcane plants as determined with an antibiotic-resistant mutant. Plant Disease 78: 241-247Google Scholar
  28. Rott P, Davis MJ and Baudin P (1994b) Serological variability in Xanthomonas albilineans, causal agent of sugarcane leaf scald disease. Plant Pathology 43: 244-252Google Scholar
  29. Rott P, Soupa D, Brunet Y, Feldmann P and Letourmy P (1995) Leaf scald (Xanthomonas albilineans) incidence and its effect on yield in seven sugarcane cultivars in Guadeloupe. Plant Pathology 44: 1075-1084Google Scholar
  30. Rott P, Mohamed IS, Klett P, Soupa D, de Saint-Albin A, Feldmann P and Letourmy P (1997) Resistance to leaf scald disease is associated with limited colonization of sugarcane and wild relatives by Xanthomonas albilineans. Phytopathology 87: 1202-1213Google Scholar
  31. Rudolph K (1993) Infection of the plant by Xanthomonas. In: Swings JG and Civerolo EL (eds) Xanthomonas (pp 193-263) Chapman & Hall, LondonGoogle Scholar
  32. Saumtally S, Medan H and Autrey LJC (1996) Evolution of aerial infection of leaf scald caused by Xanthomonas albilineans (Ashby) Dowson in sugarcane. Proceedings International Society of Sugar Cane Technologists 22: 493-496Google Scholar
  33. Sordi RA and Tokeshi H (1986) Presence of Xanthomonas albilineans in guttation droplets of sugarcane and sweet corn leaves showing leaf scald disease symptoms. Sociedade dos Técnicos Açucareiros do Brasil, 4, Jul./Aug., 60-63Google Scholar
  34. Stall RE, Gottwald TR, Koizum M and Schaad NC (1993) Ecology of plant pathogenic Xanthomonads. In: Swings JG and Civerolo EL (eds) Xanthomonas (pp 265-299) Chapman & Hall, LondonGoogle Scholar
  35. Stromberg KD, Kinkel LL and Leonard KJ (1999) Relationship between phyllosphere population sizes of Xanthomonas translucens pv. translucens and bacterial leaf streak severity on wheat seedlings. Phytopathology 89: 131-135Google Scholar
  36. Walker DIT (1971) Breeding for resistance. In: Heinz JD (ed) Sugarcane Improvement Through Breeding (pp 445-502) Elsevier Science Publishers B.V., AmsterdamGoogle Scholar

Copyright information

© Kluwer Academic Publishers 2003

Authors and Affiliations

  • J.H. Daugrois
    • 1
  • V. Dumont
    • 1
  • P. Champoiseau
    • 1
  • L. Costet
    • 2
  • R. Boisne-Noc
    • 1
  • P. Rott
    • 3
  1. 1.Station de RoujolCIRAD-CAGuadeloupeFrench West Indies
  2. 2.Laboratoire de PhytopathologieCIRADSaint Pierre CedexFrance
  3. 3.UMR 385 ENSAM-INRA-CIRAD Biologie et Génétique des Interactions Plante-ParasiteMontpellier Cedex 5France

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