Biotechnology Letters

, Volume 25, Issue 13, pp 1093–1098 | Cite as

l-Lactic acid production from raw cassava starch in a circulating loop bioreactor with cells immobilized in loofa (Luffa cylindrica)

  • Noel D. Roble
  • James C. OgbonnaEmail author
  • Hideo Tanaka


l-Lactic acid was produced from raw cassava starch, by simultaneous enzyme production, starch saccharification and fermentation in a circulating loop bioreactor with Aspergillus awamori and Lactococcus lactis spp. lactis immobilized in loofa sponge. A. awamori was immobilized directly in cylindrical loofa sponge while the L. lactis was immobilized in a loofa sponge alginate gel cube. In the loofa sponge alginate gel cube, the sponge serves as skeletal support for the gel with the cells. The alginate gel formed a hard outer layer covering the soft porous gel inside. By controlling the rate and frequency of broth circulation between the riser and downcomer columns, the riser could be maintained under aerobic condition while the downcomer was under anaerobic condition. Repeated fed-batch l-lactic acid production was performed for more than 400 h and the average lactic acid yield and productivity from raw cassava starch were 0.76 g lactic acid g−1 starch and 1.6 g lactic acid l−1 h−1, respectively.

cell immobilization circulating loop bioreactor lactic acid loofa sponge raw cassava starch 


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  1. Dalili M, Chau PC (1987) Intraparticle diffusional effects in immobilized cell particles. Appl. Microbiol. Biotechnol. 26: 500–506.Google Scholar
  2. Davidson BH, Stephanopoulos G (1985) Effect of pH oscillations on a competing mixed culture. Biotech. Bioeng. 28: 1127–1137.Google Scholar
  3. Dostàlek M, Häggström MH (1983) Mixed culture of Saccharomycopsis fibuliger and Zymomonas mobilis on Starch-use of oxygen as a regulator. Enz. Microb. Technol. 17: 269–274.Google Scholar
  4. Hofvendal K, Niel EWJ van, Hann-Häherdal B (1999) Effect of temperature and pH on growth and product formation of Lactococcus lactis ssp. lactis ATCC 19435 growing on maltose. Appl. Microbiol. Biotechnol. 51: 669–672.Google Scholar
  5. Kashket ER (1987) Bioenergetics of lactic acid bacteria: cytoplasmic pH and osmotolerance. FEMS Microbiol. Rev. 46: 233–244.Google Scholar
  6. Kurosawa H, Ishikawa H, Tanaka H (1987) L-Lactic acid production from starch by coimmobilized mixed culture system of Aspergillus awamori and Streptococcus lactis. Biotech. Bioeng. 31: 183–187.Google Scholar
  7. Mercier L, Yerushalmi D, Dochain D (1992) Kinetics of lactic acid fermentation on glucose and corn by Lactobacillus amylophilus. 55: 111–121.Google Scholar
  8. Ogbonna JC, Amano Y, Nakamura K (1989) Elucidation of optimum conditions for immobilization of viable cells in calcium alginate. J. Ferment. Bioeng. 67: 92–96.Google Scholar
  9. Ogbonna JC, Tomiyama S, Tanaka H (1996) Development of a method for immobilization of non-flocculating cells in loofa (Luffa cylidrica) sponge. Process Biochem. 31: 737–744.Google Scholar
  10. Roble ND, Ogbonna JC, Tanaka H (2003) A novel circulating loop bioreactor with cells immobilized in loofa (Luffa cylindrica) for the bioconversion of raw cassava starch to ethanol. Appl. Microbiol. Biotechnol. 60: 671–678.Google Scholar
  11. Shamala TR, Sreekantiah KR (1987) Degradation of starchy substrate by a crude enzyme preparation and utilization of the hydrolysates for lactic acid fermentation. Enz Microb. Technol. 9: 726–729.Google Scholar
  12. Vickroy TB (1985) Lactic acid. In: Blanch HW, Drew S, Wang DIC, eds. The Practice of Biotechnology: Commodity Products, Vol. 3. Elmsford, NY: Pergamon, pp. 761–776.Google Scholar

Copyright information

© Kluwer Academic Publishers 2003

Authors and Affiliations

  • Noel D. Roble
    • 1
  • James C. Ogbonna
    • 1
  • Hideo Tanaka
    • 1
  1. 1.Institute of Applied BiochemistryUniversity of TsukubaTsukuba, IbarakiJapan

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