Molecular and Cellular Biochemistry

, Volume 247, Issue 1–2, pp 205–210

Effect of chlorogenic acid on hydroxyl radical

  • Lun-Yi Zhang
  • Greg Cosma
  • Henry Gardner
  • Val Vallyathan
  • Vince Castranova
Article

DOI: 10.1023/A:1024103428348

Cite this article as:
Zhang, LY., Cosma, G., Gardner, H. et al. Mol Cell Biochem (2003) 247: 205. doi:10.1023/A:1024103428348

Abstract

Chlorogenic acid (CGA) is considered to act as an antioxidant. However, the inhibitory effects of CGA on specific radical species are not well understood. Electron spin resonance (ESR) in combination with spin trapping techniques was utilized to detect free radicals. 5,5-Dimethyl-1-pyrroline-N-oxide (DMPO) was used as a spin trapping reagent while the Fenton reaction was used as a source of hydroxyl radical (·OH). We found that CGA scavenges ·OH in a dose-dependent manner. The kinetic parameters, IC50 and Vmax, for CGA scavenging of ·OH were 110 and 1.27 μM/sec, respectively. The rate constant for the scavenging of ·OH by CGA was 7.73 × 109 M−1 sec−1. Our studies suggest that the antioxidant properties of CGA may involve a direct scavenging effect of CGA on ·OH.

chlorogenic acid hydroxyl radical ESR spin trapping 

Copyright information

© Kluwer Academic Publishers 2003

Authors and Affiliations

  • Lun-Yi Zhang
    • 1
  • Greg Cosma
    • 2
  • Henry Gardner
    • 2
  • Val Vallyathan
    • 1
  • Vince Castranova
    • 1
  1. 1.Exposure Assessment Branch and Pathology and Phsiology Research Branch, Health Effects Laboratory DivisionNational Institute for Occupational Safety and HealthMorgantownUSA
  2. 2.Colorado State UniversityFort CollinsUSA

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