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NMR experiments for the measurement of proton–proton and carbon–carbon residual dipolar couplings in uniformly labelled oligosaccharides

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Abstract

A 2D-HSQC-carbon selective/proton selective-constant time COSY, 2D-HSQC-(sel C, sel H)-CT COSY experiment, which is applicable to uniformly 13C isotopically enriched samples (U-13C) of oligosaccharides or oligonucleotides is proposed for the measurement of proton–proton RDC in crowded regions of 2D-spectra. In addition, a heteronuclear constant time-COSY experiment, 13C-13C CT-COSY, is proposed for the measurement of one bond carbon–carbon RDC in these molecules. These two methods provide an extension, to U-13C molecules, of the original homonuclear constant time-COSY experiment proposed by Tian et al. (1999) for saccharides. The combination of a number of these RDC with NOE data may provide the method of choice to study oligosaccharide conformation in the free and receptor-bound state.

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Authors and Affiliations

  1. Laboratorio Integral de Estructura de Biomoléculas Jose. R. Carracido, Unidade de Resonancia Magnética, RIAIDT, Universidad de Santiago de Compostela, 15782, Santiago de Compostela, Spain

    Manuel Martin-Pastor

  2. Departamento de Estructura y función de proteínas, Centro de Investigaciones Biológicas, CSIC, Ramiro de Maeztu 9, 28040, Madrid, Spain

    Angeles Canales-Mayordomo & Jesús Jiménez-Barbero

Authors
  1. Manuel Martin-Pastor
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  2. Angeles Canales-Mayordomo
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  3. Jesús Jiménez-Barbero
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Correspondence to Manuel Martin-Pastor.

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Martin-Pastor, M., Canales-Mayordomo, A. & Jiménez-Barbero, J. NMR experiments for the measurement of proton–proton and carbon–carbon residual dipolar couplings in uniformly labelled oligosaccharides. J Biomol NMR 26, 345–353 (2003). https://doi.org/10.1023/A:1024096807537

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