Biotechnology Letters

, Volume 25, Issue 12, pp 919–925 | Cite as

Formation of enterovirus-like particle aggregates by recombinant baculoviruses co-expressing P1 and 3CD in insect cells

  • Yu-Chen HuEmail author
  • John Tsu-An Hsu
  • Jen-Huang Huang
  • Mei-Shang Ho
  • Yi-Chen Ho


The assembly of enterovirus requires the cleavage of P1 polyprotein by protease 3CD into individual structural proteins. Two recombinant baculoviruses were constructed to encode P1 and 3CD of enterovirus 71 (EV71), respectively. The expressed 3CD successfully cleaved P1 in vitro and in vivo. Also, the co-infection in insect cells resulted in crystalline virus-like particle structures morphologically resembling the authentic EV71 aggregates, which are reported for the first time.

baculovirus enterovirus 71 insect cell virus aggregation virus-like particle 


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Copyright information

© Kluwer Academic Publishers 2003

Authors and Affiliations

  • Yu-Chen Hu
    • 1
    Email author
  • John Tsu-An Hsu
    • 1
    • 2
  • Jen-Huang Huang
    • 1
  • Mei-Shang Ho
    • 3
  • Yi-Chen Ho
    • 1
  1. 1.Department of Chemical EngineeringNational Tsing Hua UniversityHsinchuTaiwan
  2. 2.Division of Biotechnology and Pharmaceutical ResearchNational Health Research InstitutesTaiwan
  3. 3.Academia SinicaInstitute of Biomedical SciencesTaipeiTaiwan

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