Veterinary Research Communications

, Volume 27, Issue 4, pp 275–280 | Cite as

Reduction of Salmonella in Gnotobiotic Japanese Quails Caused by the Enterocin A-producing EK13 Strain of Enterococcus faecium

  • A. Lauková
  • P. Guba
  • R. Nemcová
  • Z. Vasilková
Article

Abstract

The effect of the enterocin A-producing EK strain of Enterococcus faecium on Salmonella dusseldorf SA31 was tested in gnotobiotic Japanese quails. Sixteen 3-day-old gnotobiotic Japanese quails, hatched from disinfected eggs placed in sterile boxes, were divided into two groups of 8 birds: a control group, which was inoculated orally with the SA31 strain (1×107 CFU/ml), and the experimental group, which was inoculated orally with 200 μl of E. faecium (1×109 CFU/ml), 16 h before infection with the S. dusseldorf. The latter group then received the same average dose of E. faecium daily in drinking water. Faecal samples were taken 8, 24, 48 and 168 h after the inoculation of S. dusseldorf and examined for S. dusseldorf and E. faecium (EK13). The quails were then killed and the number of the EK13 strain of E. faecium and of S. dusseldorf in the caecum and ileum were estimated. A reducing effect of the EK13 strain against the SA31 strain in faeces was detected in the samples taken at 24 and 48 h from the group with the EK13 strain. Significant reductions were also found in the numbers of S. dusseldorf SA31 strain in the caecum but not in the ileum.

bacteriocin enterocin Enterococcus faecium gnotobiotic Japanese quail probiotic Salmonella dusseldorf 

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Copyright information

© Kluwer Academic Publishers 2003

Authors and Affiliations

  • A. Lauková
    • 1
  • P. Guba
    • 2
  • R. Nemcová
    • 2
  • Z. Vasilková
    • 3
  1. 1.Institute of Animal Physiology Slovak Academy of SciencesKošiceSlovakia
  2. 2.Research Institute of Veterinary MedicineUniversity of Veterinary Medicine, Department of Gnotobiology and Diseases of YoungKošiceSlovakia
  3. 3.Parasitological Institute of Slovak Academy of SciencesKošiceSlovakia

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