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Screening filamentous fungi isolated from estuarine sediments for the ability to oxidize polycyclic aromatic hydrocarbons

  • Manuela da Silva
  • Carl E. Cerniglia
  • Jairaj V. Pothuluri
  • Vanderlei P. Canhos
  • Elisa Esposito
Article

Abstract

Nineteen filamentous fungi, isolated from estuarine sediments in Brazil, were screened for degradation of polycyclic aromatic hydrocarbons (PAH). The fungal isolates were incubated with pyrene. The cultures were extracted and metabolites in the extracts were detected by high performance liquid chromatography (HPLC) and u.v. spectral analyses. Six fungi were selected for further studies using [4,5,9,10-14C]pyrene. Cyclothyrium sp., Penicillium simplicissimum, Psilocybe sp., and a sterile mycelium demonstrated the ability to transform pyrene. Cyclothyrium sp. was the most efficient fungus, transforming 48% of pyrene to pyrene trans-4,5-dihydrodiol, pyrene-1,6-quinone, pyrene-1,8-quinone and 1-hydroxypyrene. This fungus was also evaluated with a synthetic mixture of PAH. After 192 h of incubation, Cyclothyrium sp. was able to degrade simultaneously 70, 74, 59 and 38% of phenanthrene, pyrene, anthracene and benzo[a]pyrene, respectively.

Bioremediation filamentous fungi mycoremediation PAH mixture pyrene 

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Copyright information

© Kluwer Academic Publishers 2003

Authors and Affiliations

  • Manuela da Silva
    • 1
  • Carl E. Cerniglia
    • 2
  • Jairaj V. Pothuluri
    • 2
  • Vanderlei P. Canhos
    • 3
  • Elisa Esposito
    • 4
  1. 1.Department of Food ScienceSchool of Food Engineering, State University of CampinasCampinas, São PauloBrazil; Tel.:
  2. 2.Department of MicrobiologyINCQS/Fundação Oswaldo CruzRio de JaneiroBrazil
  3. 3.Department of Food ScienceSchool of Food Engineering, State University of CampinasCampinas, São PauloBrazil
  4. 4.Department of Environmental ScienceUniversity of Mogi das CruzesMogi das Cruzes, São PauloBrazil

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