Technological aspects of extractive fermentation using aqueous two-phase systems

  • R.M. Banik
  • A. Santhiagu
  • B. Kanari
  • C. Sabarinath
  • S.N. Upadhyay
Article
Aqueous two-phase systems,extractive fermentation in situ fermentation in situ product recovery polyethylene glycol 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Abbot, N.L., Blankschtein, D. & Hatton, T.A. 1990 On protein partitioning in aqueous polymer systems. Bioseparation 20, 421–423.Google Scholar
  2. Alam, S., Weigand, W.A. & Hong, J. 1989 On the mechanism of growth of cells in the mixed aqueous two-phase systems. Applied Biochemistry and Biotechnology 20, 421–423.Google Scholar
  3. Albertsson, P.A. 1971 Partition of Cell Particles and Macromolecules. 2nd edn. New York: Wiley-Interscience, ISBN 0471020478.Google Scholar
  4. Albertsson, P.A. 1986 Partition of Cell Particles and Macromolecules 3rd edn. New York: Wiley-Interscience, ISBN 0471828203.Google Scholar
  5. Andersson, E., Johansson, A. & Hahn-Haegerdal, B. 1985 ?-Amylase production in aqueous two-phase system with Bacillus subtilis. Enzyme and Microbial Technology 7, 333–338.Google Scholar
  6. Antov, M.G. & Pericin, D.M. 2000 Production of pectinases by Polyporus squamosus in aqueous two-phase system. Enzyme and Microbial Technology 28, 467–472.Google Scholar
  7. Arnold, S.G., Mclellan, P.G. & Daugulis, A.J. 1996 Dynamic modelling and performance optimization of extractive fermentation. Canadian Journal of Chemical Engineering 74(3), 385–393.Google Scholar
  8. Banik, R.M. 1997 Aqueous two-phase system for enzyme purification from microorganisms. Trends in Microbial Exploitation 1, 234–240.Google Scholar
  9. Bordier, C. 1981 Phase separation of integral membrane proteins in Triton X-114 solution. Journal of Biological Chemistry 256, 1604–1607.Google Scholar
  10. Chang, H.N., Lee, Y.H. & Lee, C.Y. 1992 Continuous production of 6-APA in an aqueous two-phase system. Annals of New York Academy of Sciences 672, 643–648.Google Scholar
  11. Chen, J.P. & Lee, M.S. 1994 Simultaneous production and partition of chitinase during growth of Serratia marcescens in an aqueous two-phase system. Biotechnology 8, 783–788.Google Scholar
  12. Chen, J.P. & Lee, M.S. 1995 Enhanced production of Serratia marcescens chitinase in PEG/dextran aqueous two-phase systems. Enzyme and Microbial Technology 17, 1021–1027.Google Scholar
  13. Chen, J.P. 1992 Partition and separation of ?-lactalbumin and ?-lactalbumin in PEG-potassium phosphate aqueous two-phase system. Journal of Fermentation and Bioengineering 73, 140–147.Google Scholar
  14. Cunha, M.T., Tjerneld, F., Cabral, J.M.S. & Aires-Barros, M.S. 1998 Effect of electrolytes and surfactants on thermoseparation of an ethylene oxide-propylene oxide random copolymer in aqueous solution. Journal of Chromatography B 711, 53–60.Google Scholar
  15. Diamond, A.D. & Hsu, J.T. 1992 Aqueous two-phase systems for biomolecule separation. Advances in Biochemical Engineering and Biotechnology 47, 89–135.Google Scholar
  16. Drouin, C.M. & Cooper, D.G. 1992 Biosurfactants in aqueous two-phase fermentation. Biotechnology and Bioengineering 40, 86–90.Google Scholar
  17. Flanagan, S.D. & Barondes, S.H. 1975 Affinity partitioning: a method for purification of proteins using specific polymer ligand in aqueous polymer two-phase system. Journal of Biological Chemistry 250, 1484–1489.Google Scholar
  18. Flygare, S., Wilkstrom, P., Johansson, G. & Larsson, P-O. 1990 Magnetic aqueous two-phase separations in preparative applications. Enzyme and Microbial Technology 12, 95–103.Google Scholar
  19. Frakas, T., Stalbrand, H. & Tjerneld, F. 1996 Partitioning of bmannanase and a-galactosidase from Aspergillus niger in Ucon/ Reppal aqueous two-phase systems and temperature induced phase separation. Bioseparation 6, 147–157.Google Scholar
  20. Gobole, P.P., Tsai, R-S. & Clark, W.M. 1991 Affinity partitioning of vancomycin. Biotechnology and Bioengineering 38, 535–544.Google Scholar
  21. Harris, P.A., Karlstrom, G. & Tjerneld, F. 1991 Enzyme purification using temperature induced phase formation. Bioseparation 2, 237–246.Google Scholar
  22. Honda, K., Maeda, Y., Sasakwa, S., Ohno, H. & Hsutida, E. 1981 The components contained in polyethylene glycol of commercial grade (PEG 6000) as cell fusogen. Biochemical and Biophysical Research Communications 100, 165–171.Google Scholar
  23. Hotha, S. & Banik, R.M. 1997 Production of alkaline protease by Bacillus thuringiensis H 14 in aqueous two-phase systems. Journal of Chemical Technology and Biotechnology 69, 5–10.Google Scholar
  24. Hughes, P. & Lowe, C.R. 1988 Purification of proteins by aqueous two-phase partition in novel acrylic copolymer systems. Enzyme and Microbial Technology 10, 115–122.Google Scholar
  25. Hustedt, H. 1986 Extractive recovery with simple recycling of phase forming chemicals. Biotechnology Letters 8, 791–796.Google Scholar
  26. Hustedt, H., Kroner, K.H., Stach, W. & Kula, M.R. 1978 Procedure for the simultaneous large scale isolation of pullulanase and 1,4-aglucan phosphorylase from Klebsiella pneumoniae involving liquid-liquid separations. Biotechnology and Bioengineering 20, 1989–2005.Google Scholar
  27. Jiang, N.Q. & Zhao, F.S. 1999 L-Asparaginase release from Escherichia coli cells with aqueous two-phase systems. Industrial Microbiology 29(2), 5–9.Google Scholar
  28. Johansson, G. 1970 Studies on aqueous dextran-polyethylene glycol two-phase systems containing charged polyethylene glycol. Biochemica Biophysica Acta 222, 381–389.Google Scholar
  29. Johansson, G. 1994 Partitioning procedures and techniques: small molecules and macromolecules. Methods in Enzymology 228, 28–42.Google Scholar
  30. Johansson, G., Kopperschlager, G. & Albertsson, P.A. 1983 Affinity partitioning of phosphofructokinase from baker's yeast using polymer based Cibacron-blue F3GA. European Journal of Biochemistry 131, 589–594.Google Scholar
  31. Johansson, G. & Tjerneld, F. 1989 Affinity partition between aqueous two-phase system: a tool for large scale purification of enzymes. Journal of Biotechnology 11, 133–142.Google Scholar
  32. Kannan, R. 1997 Studies on extractive fermentation of Bacillus lichiniformis alpha amylase using PEG-citrate aqueous two-phase system. M.Tech Thesis, School of Biochemical Engineering, IT, BHU, Varanasi, India.Google Scholar
  33. Katzbauer, B., Cesi, V., Narodoslawsky, M. & Moser, A. 1995 Extractive lactic acid fermentation using aqueous two-phase system. Chemical and Biochemical Engineering Quest 9, 79–87.Google Scholar
  34. Kaul, A., Pereira, R.A.M., Asenjo, J.A. & Merchuk, J.C. 1995 Kinetics of phase separation for polyethylene glycol-phosphate two-phase systems. Biotechnology and Bioengineering 48, 246–256.Google Scholar
  35. Kroner, K.H., Husedt, H. & Kula, M.R. 1982 Evaluation of crude dextran as a phase forming polymer for extraction of enzymes in aqueous two-phase system in large scale. Biotechnology and Bioengineering 24, 1015–1045.Google Scholar
  36. Kuboi, R., Maruki, T., Tanaka, H. & Komasawa, I. 1994 Fermentation of Bacillus subtilis ATCC 6633 and production of subtilin in polyethylene glycol/phosphate aqueous two-phase systems. Journal of Fermentation and Bioengineering 78, 431–436.Google Scholar
  37. Kuboi, R., Umakoshi, H. & Komasawa, I. 1995 Extractive cultivation of Escherichia coli using polyethylene glycol/phosphate aqueous two-phase systems to produce b-galactosidase. Biotechnology Progress 11, 202–207.Google Scholar
  38. Kuhn, I. 1980 Alcoholic fermentation in an aqueous two-phase system. Biotechnology and Bioengineering 22, 2393–2398.Google Scholar
  39. Kula, M.R., Kroner, K.H. & Hustedt, H. 1982 Purification of enzymes by liquid-liquid extraction. In Advances in Biochemical Engineering, ed. Fiechter, vol. 24. pp. 74-118. Springer-Verlag. ISBN-0-387-11018-6.Google Scholar
  40. Kulkarni, N., Vaidya, A. & Rao, M. 1999 Extractive cultivation of recombinant Escherichia coli using aqueous two-phase system for production and separation of extracellular xylanase. Biochemical and Biophysical Research Communications 225, 274–278.Google Scholar
  41. Kwon, Y.J., Kaul, R. & Mattiasson, B. 1996 Extractive lactic acid fermentation in polyethyleneimine based aqueous two-phase system. Biotechnology and Bioengineering 50, 280–290.Google Scholar
  42. Larsson, P.O. 1994 Magnetically enhanced phase separation. Methods in Enzymology 228, 112–117.Google Scholar
  43. Lee, Y.H. & Chang, H.N. 1989 Biosynthesis of acrylamide from acrylonitrile in aqueous two-phase system. Biotechnology Letters 11, 23–26.Google Scholar
  44. Lee, Y.H. & Chang, H.N. 1990 Production of alkaline protease by Bacillus lichiniformis in an aqueous two-phase system. Journal of Fermentation and Bioengineering 2, 89–92.Google Scholar
  45. Li, C., Ouyang, F. & Bai, J. 2000 Extractive cultivation of Lactococcus lactis using a polyethylene glycol/MgSO4·7H2O aqueous two-phase system to produce nisin. Biotechnology Letters 22, 843–847.Google Scholar
  46. Liu, C.L., Kamei, D.T., King, J.A., Wang, D.I.C. & Blankschtein, D. 1998 Separation of proteins and virusus using aqueous two-phase micellar systems. Journal of Chromatography B 711, 127–138.Google Scholar
  47. Lius, L.L., Nikas, Y. & Blankschtein, D. 1995 Partitioning of proteins using two-phase aqueous surfactant systems. AIChE Journal 41, 991–995.Google Scholar
  48. Luong, J.H.T. & Nguyen, A.L. 1990 Affinity partitioning of bioproducts. Biotechnology 8, 306–307.Google Scholar
  49. Luther, J.H.T. & Glatz, C.E. 1993 Using genetic engineering to enhance protein recovery in aqueous two-phase system. Abstract papers of American Chemical Society 205 meet, pt 1, BIOT95.Google Scholar
  50. Mattiasson, B., Ramstorp, M. & Ling, G.I. 1982 Partition affinity ligand assay (PALA): application in the analysis of lapteus, macromolecules and cells. Advances in Applied Microbiology 28, 117–147.Google Scholar
  51. Morris, C.J.O.R. & Morris, P. 1976 Separation Methods in Biochemistry, 2nd edn. London: Pitman. ISBN 0-470-61579-6.Google Scholar
  52. Noble, M.J. & Varley, J. 1999 Collodial gas aphrons generated from the anionic surfactant AOT for the separation of proteins from aqueous solution. Journal Chemical Technology and Biotechnology 74, 231–237.Google Scholar
  53. Persson, I., Nystrom, L., Ageland, H. & Tjerneld, F. 1999 Purification of recombinant proteins using thermoreversible aqueous twophase system and polymer recycling. Journal Chemical Technology and Biotechnology 74, 238–243.Google Scholar
  54. Persson, I., Tjerneld, F. & Hahn-Haegerdal, B. 1984 Semicontinuous cellulase production in an aqueous two-phase system with Trichoderma reesei Rutgers C 30. Enzyme and Microbial Technology 6, 415–418.Google Scholar
  55. Persson, I., Tjerneld, F. & Hahn-Hagerdal, B. 1989 Production of bglucosidase with Aspergillus niger QM329 in aqueous two-phase systems. Biotechnology Techniques 3, 265–270.Google Scholar
  56. Pietruszka, N., Galev, I. Yu., Kumar, A., Brzozowski, K. & Mattiasson, B. 2000 New polymers forming aqueous two-phase polymer systems. Biotechnology Progress 16, 408–415.Google Scholar
  57. Planas, J., Lefebvre, D., Tjerneld, F. & Hahn-Haegerdal, B. 1997 Analysis of phase composition in an aqueous two-phase system using a two column chromatographic method: application to lactic acid production by extractive fermentation. Biotechnology and Bioengineering 54, 303–311.Google Scholar
  58. Planas, J., Radstom, P., Tjerneld, F. & Hahn-Haegerdal, B. 1995 A novel aqueous two-phase system for the production of lactic acid with extractive fermentation. Pharmacology and Toxicology supplementary 2, 52.Google Scholar
  59. Planas, J., Radstom, P., Tjerneld, F. & Hahn-Haegerdal, B. 1996 Enhanced production of lactic acid through the use of a novel aqueous two-phase system as an extractive fermentation system. Applied Microbiology and Biotechnology 45, 737–743.Google Scholar
  60. Puziss, M. & Heden, C.G. 1965 Toxin production by Clostridium tetani in biphasic liquid mixtures. Biotechnology and Bioengineering 7, 355–366.Google Scholar
  61. Raghavarao, K.S.M.S., Stewart, R.M., Rudge, S.R. & Todd, P. 1998 Electrokinetic demixing of aqueous two-phase systems. III. Drop electrophoretic mobilities and demixing rates. Biotechnology Progress 14, 922–930.Google Scholar
  62. Ramadas, M., Halst, O. & Mattiasson, B. 1996 Production of amyloglucosidase by Aspergillus niger under different cultivation regimens. World Journal of Microbiology and Biotechnology 12, 267–271.Google Scholar
  63. Rito-Palomares, M., Negrete, A., Miranda, L., Flores, C., Galindo, E. & Serrano-Carreon, L. 2001 The potential application of aqueous two-phase systems for in situ recovery of 6-pentyl-?-pyrone produced by Trichoderma harzianum. Enzyme and Microbial Technology 28, 625–631.Google Scholar
  64. Sarmento, M.J., Pires, M.J., Cabral, M.S. & Aires-Barros, M.R. 1994 Liquid-liquid extraction of recombinant protein, cytochrome-b5, with aqueous two-phase systems of polyethylene glycol and potassium phosphate salts. Journal of Chromatography 668, 717–720.Google Scholar
  65. Srinivas, N.D., Barhate, R.S., Raghavarao, K.S.M.S. & Todd, P. 2000 Acoustic demixing of aqueous two-phase systems. Applied Microbiology and Biotechnology 53, 650–654.Google Scholar
  66. Stocks, S.J. & Brookes, D.E. 1988 Development of a general ligand for immunoaffinity partitioning in aqueous two-phase system. Analytical Biochemistry 173, 86–92.Google Scholar
  67. Stredansky, M., Svore, R., Sturdik, T. & Dercova, K. 1993 Repeated batch a-amylase production in aqueous two-phase system with Bacillus strains. Journal of Biotechnology 27, 181–191.Google Scholar
  68. Suh, S.S., Vandam, M., Wuenschell, G., Plunkett, S. & Arnold, F.H. 1989 Novel metal affinity protein separations. Abstracts of Papers of the American Chemical Society 1989 meeting, MBID50. Google Scholar
  69. Taguchi, F., Yamada, K., Hasegawa, K., Taki-Saito, T. & Hara, K. 1996 Continuous hydrogen production by Clostridium species strain no. 2 from cellulose hydrolysate in an aqueous two-phase system. Journal of Fermentation and Bioengineering 82, 80–83.Google Scholar
  70. Tjerneld, F., Berner, S., Cajarville, A. & Johansson, G. 1986 New aqueous two-phase system based on hydroxypropyl starch useful in enzyme purification. Enzyme and Microbial Technology 8, 417–423.Google Scholar
  71. Tomaska, M., Stredansky, M., Tomaskova, A. & Sturdik, E. 1995 Lactose hydrolysis in aqueous two phase system by whole cell betagalactosidase of Kluyveromyces marxianus. Bioprocess Engineering 12, 17–20.Google Scholar
  72. Van Berlo, M., Ottens, M., Luyben, K.Ch.A.M. & Van der Wielen, L.A.M. 2000 Feasible boundaries of aqueous two-phase systems with NH3 and CO2 as recyclable volatile salts. Biotechnology and Bioengineering 70, 65–71.Google Scholar
  73. Van Berlo, M., Luyben, K.Ch.A.M. & Van der Wielen, L.A.M. 1998 Polyethylene-salt aqueous two-phase systems with easily recyclable volatile salts. Journal of Chromatography 711, 61–68.Google Scholar
  74. Van der Pol, L.A., Paijens, I. & Tramper, J. 1995 Dextran as protectant against damage caused by sparging by hybridoma cells in a bubble column. Journal of Biotechnology 43, 103–110.Google Scholar
  75. Van-Sonsbeer, H.M., Beeftink, H.H. & Tramper, J. 1993 Two liquidphase bioreactors. Enzyme and Microbial Technology 15, 722–729.Google Scholar
  76. Vernau, J. & Kula, M.R. 1990 Extraction of proteins from biological raw material using aqueous polyethylene glycol-citrate phase systems. Biotechnology and Applied Biochemistry 12, 397–404.Google Scholar
  77. Vikrostrom, P., Flygare, S., Grondalen, A. & Larsson, P.O. 1987 Magnetic aqueous two-phase separation: a new technique to increase rate of phase separation, using dextran-ferrofluid or larger iron oxide particles. Analytical Biochemistry 167, 331–339.Google Scholar
  78. Zhao, F., Wu, J. & Liao, H. 1995 Bioconversion of acrylonitrile to acrylamide in aqueous two-phase system. Industrial Microbiology 25, 6–12.Google Scholar
  79. Zijlstra, G.M., Michielsen, M.J., De-Gooijer, C.D., Vanderpol, L.A. & Tramper, J. 1996 Hybridoma and CHO cell partitioning in aqueous two-phase systems. Biotechnology Progress 12, 363–370.Google Scholar
  80. Zijlstra, G.M., Michielsen, M.J., De-Gooijer, C.D., Vanderpol, L.A. & Tramper, J. 1998 IgG and hybridoma partitioning in aqueous twophase systems containing a dye-ligand. Enzyme and Microbial Technology 7, 117–126.Google Scholar

Copyright information

© Kluwer Academic Publishers 2003

Authors and Affiliations

  • R.M. Banik
    • 1
  • A. Santhiagu
    • 1
  • B. Kanari
    • 1
  • C. Sabarinath
    • 1
  • S.N. Upadhyay
    • 1
  1. 1.School of Biochemical Engineering,Institute of Technology,Banaras Hindu UniversityVaranasiIndia

Personalised recommendations