Cytotechnology

, Volume 40, Issue 1–3, pp 139–149 | Cite as

Protective mechanism of reduced water against alloxan-induced pancreatic β-cell damage: Scavenging effect against reactive oxygen species

  • Yuping Li
  • Tomohiro Nishimura
  • Kiichiro Teruya
  • Tei Maki
  • Takaaki Komatsu
  • Takeki Hamasaki
  • Taichi Kashiwagi
  • Shigeru Kabayama
  • Sun-Yup Shim
  • Yoshinori Katakura
  • Kazuhiro Osada
  • Takeshi Kawahara
  • Kazumichi Otsubo
  • Shinkatsu Morisawa
  • Yoshitoki Ishii
  • Zbigniew Gadek
  • Sanetaka Shirahata
Article

Abstract

Reactive oxygen species (ROS) cause irreversible damage to biological macromolecules, resulting in many diseases. Reduced water (RW) such as hydrogen-rich electrolyzed reduced water and natural reduced waters like Hita Tenryosui water in Japan and Nordenau water in Germany that are known to improve various diseases, could protect a hamster pancreatic β cell line, HIT-T15 from alloxan-induced cell damage. Alloxan, a diabetogenic compound, is used to induce type 1 diabetes mellitus in animals. Its diabetogenic effect is exerted via the production of ROS. Alloxan-treated HIT-T15 cells exhibited lowered viability, increased intracellular ROS levels, elevated cytosolic free Ca2+ concentration, DNA fragmentation, decreased intracellular ATP levels and lowering of glucose-stimulated release of insulin. RW completely prevented the generation of alloxan-induced ROS, increase of cytosolic Ca2+ concentration, decrease of intracellular ATP level, and lowering of glucose-stimulated insulin release, and strongly blocked DNA fragmentation, partially suppressing the lowering of viability of alloxan-treated cells. Intracellular ATP levels and glucose-stimulated insulin secretion were increased by RW to 2–3.5 times and 2–4 times, respectively, suggesting that RW enhances the glucose-sensitivity and glucose response of β-cells. The protective activity of RW was stable at 4 °C for over a month, but was lost by autoclaving. These results suggest that RW protects pancreatic β-cells from alloxan-induced cell damage by preventing alloxan-derived ROS generation. RW may be useful in preventing alloxan-induced type 1-diabetes mellitus.

alloxan apoptosis diabetes HIT-T15 cells insulin pancreatic beta cells reactive oxygen species reduced water 

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Copyright information

© Kluwer Academic Publishers 2002

Authors and Affiliations

  • Yuping Li
    • 1
  • Tomohiro Nishimura
    • 1
  • Kiichiro Teruya
    • 1
  • Tei Maki
    • 1
  • Takaaki Komatsu
    • 1
  • Takeki Hamasaki
    • 1
  • Taichi Kashiwagi
    • 1
  • Shigeru Kabayama
    • 2
  • Sun-Yup Shim
    • 1
  • Yoshinori Katakura
    • 1
  • Kazuhiro Osada
    • 1
  • Takeshi Kawahara
    • 1
  • Kazumichi Otsubo
    • 2
  • Shinkatsu Morisawa
    • 2
  • Yoshitoki Ishii
    • 3
  • Zbigniew Gadek
    • 4
  • Sanetaka Shirahata
    • 1
  1. 1.Department of Genetic Resources Technology, Faculty of AgricultureKyushu UniversityHigashi-ku, FukuokaJapan
  2. 2.Nihon Trim Co. Ltd.Kita-ku, OsakaJapan
  3. 3.Hita Tenryosui Co. Ltd.Hita, OitaJapan
  4. 4.Center for Holistic Medicine and NaturopathySchmallenberg-NordenauGermany

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