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Pharmaceutical Research

, Volume 20, Issue 6, pp 864–872 | Cite as

Prediction of in Vivo Tissue Distribution from in Vitro Data. 2. Correlation Between in Vitro and in Vivo Tissue Distribution of a Homologous Series of Nine 5-n-Alkyl-5-Ethyl Barbituric Acids

  • Peter BallardEmail author
  • David E. Leahy
  • Malcolm Rowland
Article

Abstract

Purpose. To evaluate the ability to determine accurate in vivo tissue-to-unbound plasma distribution coefficients (Kpue) from in vitro data.

Methods. Fresh pieces of fifteen rat tissues/organs were incubated at 37°C with a homologous series of nine barbiturates covering a wide range of lipophilicity (Log P 0.02 to 4.13). Steady-state in vivoKpue values were estimated from the tissue and plasma concentrations following simultaneous dosing by constant rate i.v. infusion of all nine barbiturates. Drug concentrations in the tissues and media were determined by HPLC with UV or mass spectrometric detection.

Results. The pharmacokinetics of the barbiturate series following constant rate i.v. infusion indicated a range of clearance (0.49 to 30 ml.min\-1.kg\-1) and volume of distribution at steady state (0.51 to 1.9 l.kg\-1) values. Good agreement was observed between the in vitro and in vivoKpu values, although for the most lipophilic barbiturates the in vitro data underpredicted the in vivo tissue distribution for all tissues.

Conclusions. The in vitro system for predicting the extent of in vivo tissue distribution works well for compounds of widely differing lipophilicity, although for the most lipophilic drugs it may result in an underprediction of in vivo values.

in vitro-in vivo correlation barbiturate tissue distribution pharmacokinetics 

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Copyright information

© Plenum Publishing Corporation 2003

Authors and Affiliations

  • Peter Ballard
    • 1
    Email author
  • David E. Leahy
    • 2
  • Malcolm Rowland
    • 3
  1. 1.Discovery-DMPK, Mereside, AstraZenecaAlderley Park, CheshireUnited Kingdom
  2. 2.CyprotexMacclesfield, CheshireUnited Kingdom
  3. 3.dSchool of Pharmacy and Pharmaceutical SciencesUniversity of ManchesterManchesterUnited Kingdom

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