, Volume 27, Issue 3, pp 137–145 | Cite as

Alveolar Epithelial Cell-Macrophage Interactions Affect Oxygen-Stimulated Interleukin-8 Release

  • Matthew R. Hjort
  • Andrew J. Brenyo
  • Jacob N. Finkelstein
  • Mark W. Frampton
  • Michael B. LoMonaco
  • Judith C. Stewart
  • Carl J. Johnston
  • Carl T. D'Angio


Interactions of alveolar macrophages with respiratory epithelium may play a key role in hyperoxia-induced lung inflammation. We studied the effect of cell–cell contact with epithelial cells in hyperoxia on macrophages' secretion of interleukin-8 (IL-8). A549 pulmonary epithelial cells and THP-1 monocyte/macrophage cells were cultured either singly, in contact coculture, or prevented from contact by a porous membrane, and exposed to oxygen or room air. Phorbol-12-myristate-13-acetate-(PMA)-treated THP-1 cells were exposed to the same conditions. Neither cell line cultured alone produced appreciable amounts of IL-8 in hyperoxia. Contact cocultures exposed to hyperoxia produced increased IL-8, while in the noncontact coculture it was attenuated. Both cell–cell contact and PMA increased THP-1 cell CD54 expression. Intracellular IL-8 production was increased in contact cocultured, hyperoxia exposed THP-1 cells, while the A549 sample showed no change. Increased IL-8 mRNA expression was not demonstrated in cocultured, hyperoxic exposed THP-1 cells, suggesting nontranscriptional regulation of IL-8 protein levels. Contact with epithelial cells appears to potentiate macrophage responses to hyperoxia.

alveolar macrophage THP-1 A549 interleukin-8 hyperoxia 


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Copyright information

© Plenum Publishing Corporation 2003

Authors and Affiliations

  • Matthew R. Hjort
    • 1
  • Andrew J. Brenyo
    • 1
  • Jacob N. Finkelstein
    • 1
  • Mark W. Frampton
    • 2
  • Michael B. LoMonaco
    • 1
  • Judith C. Stewart
    • 2
  • Carl J. Johnston
    • 1
  • Carl T. D'Angio
    • 1
  1. 1.Division of Neonatology, Strong Children's Research CenterUniversity of RochesterRochester
  2. 2.Pulmonary and Critical Care DivisionUniversity of RochesterRochester

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