European Journal of Epidemiology

, Volume 18, Issue 4, pp 357–362 | Cite as

Investigation in central Italy of the possible association between Cryptococcus neoformans var. Gattii and Eucalyptus camaldulensis

  • E. Campisi
  • F. Mancianti
  • G. Pini
  • E. Faggi
  • G. Gargani
Article

Abstract

The authors present a worldwide review of isolations of Cryptococcus neoformans, var. neoformans and C. neoformans var. gattii from animals and vegetation, referring in particular to the already well-known association of the former variety with Eucalyptus camaldulensis. They then review the Italian situation relative to this association and their studies carried out in Central Italy: in Latina (Lazio), Pisa, Viareggio and Lake Massaciuccoli (Tuscany). From the 256 E. camaldulensis trees examined C. neoformans var. gattii was not isolated. An E. camaldulensis tree situated in the nature reserve on Lake Massaciuccoli proved to be positive for C. neoformans var. neoformans. This variety was isolated from the leaves, flowers, bark and the debris at the foot of the tree, suggesting that it had colonized the entire tree and that it was capable of developing not only on its usual habitat (bird guano, soil rich with guano) but also on Eucalyptus trees. The identity of the isolates was confirmed by their genomic profiles obtained by random amplification polymorphic DNA (RAPD) with the primer (GACA)4. The presence of a single genotype indicates a sole source of contamination, perhaps brought by a bird coming from a contaminated environment.

Cryptococcus neoformans var. gattii C. neoformans var. neoformans Eucalyptus camaldulensis Italy 

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References

  1. 1.
    Vanbreuseghem R, Takashio M. An atypical strain of Cryptococcus neoformans (Sanfelice) Vuillemin 1984. Part II. Cryptococcus neoformans var. gatti var. nov. Ann Soc Belg Med Trop 1970; 50: 695–702.Google Scholar
  2. 2.
    Kwon-Chung KJ. A new species of Filobasidiella, the sexual state of Cryptococcus neoformans B and C serotypes. Mycologia 1976; 68: 942–946.Google Scholar
  3. 3.
    Kwon-Chung KJ, Bennett JE. High prevalence of Cryptococcus neoformans var. gatti in tropical and subtropical regions. Zentralbl Bakteriol Mykrobiol Hyg 1984; 257: 213–218.Google Scholar
  4. 4.
    Ellis DH. Cryptococcus neoformans var. gattii in Australia. J Clin Microb 1987; 25: 430–431.Google Scholar
  5. 5.
    Dromer F, Ronin O, Dupont B. Isolation of Cryptococcus neoformans var. gattii from an Asian patient in France: Evidence for dormant infection in healthy subjects. J Med Vet Mycol 1992; 30: 395–397.Google Scholar
  6. 6.
    Montagna MT, Tortorano AM, Fiore L, Ingletti AM, Barbuti S. Cryptococcus neoformans var. gattii en Italie. Note I. Premier cas autochtone de méningite de sérotype B chez un sujet VIH positif. J Mycol Med 1997; 7: 90–92.Google Scholar
  7. 7.
    Malik R, Wigney DI, Muir DB, Gregory DJ, Love DN. Cryptococcosis in cats: Clinical and mycological assessment of cases and evaluation of treatment using oral administered fluconazole. J Med Vet Mycol 1992; 30: 132–144.Google Scholar
  8. 8.
    Malik R, Dill-Macky E, Martin P, Wigney DI, Muir DB, Love DN. Cryptococcosis in dogs: A retrospective study of 20 consecutive cases. J Med Vet Mycol 1995; 33: 291–297.Google Scholar
  9. 9.
    Riley CB, Bolton JR, Mills JN, Thomas JB. Cryptococcosis in seven horses. Aust Vet J 1992; 69: 135–139.Google Scholar
  10. 10.
    Baro T, Torres-Rodriguez JM, De Mendoza MH, Morera Y, Alia C. First identification of autochthonous Cryptococcus neoformans var. gattii isolated from goats with predominantly severe pulmonary diseases in Spain. J Clin Microbiol 1998; 36: 458–461.Google Scholar
  11. 11.
    Ellis DH, Pfeiffer TJ. Natural habitat of Cryptococcus neoformans var. gattii J Clin Micr 1990; 28: 1642–1644.Google Scholar
  12. 12.
    Pfeiffer TJ, Ellis DH. Environmental isolation of Cryptococcus neoformans var. gattii from California. J Infect Dis 1990; 163: 929–930.Google Scholar
  13. 13.
    Pfeiffer TJ, Ellis DH. Environmental isolation of Cryptococcus neoformans var. gattii from Eucalyptus tereticornis. J Med Vet Mycol 1992; 30: 407–408.Google Scholar
  14. 14.
    Duarte A, Ordonez N, Castaneda E. Association of yeasts of the Cryptococcus genus with Eucalyptus species in Santafe de Bogota. Rev Inst Med Trop Sao Paulo 1994; 36: 125–130.Google Scholar
  15. 15.
    Montagna MT, Viviani MA, Pulito A, et al. Cryptococcus neoformans var. gattii in Italy. Note II. Environmental investigations related to an autochthonous clinical case in Apulia. Myc Med 1997; 7: 93–96.Google Scholar
  16. 16.
    Montenegro H, Paula CR. Environmental isolation of Cryptococcus neoformans var. gattii and Cryptococcus neoformans var. neoformans in the city of São Paulo, Brasil. Med Mycol 2000; 38: 385–390.Google Scholar
  17. 17.
    Abraham M, Matthews MS, John TJ. Environmental isolation of Cryptococcus neoformans var. neoformans from Vellore. Indian J Med Res 1997; 106: 458–459.Google Scholar
  18. 18.
    Sorrell TC et al. Concordance of clinical and environmental isolates of Cryptococcus neoformans var. gattii by random amplification of polymorphic DNA analysis and PCR fingerprinting. JCM 1996; 34: 1253–1260.Google Scholar
  19. 19.
    Chen SC, Currie BJ, Campbell HM, et al. Cryptococcus neoformans var. gattii infection in northern Australia: Existence of an environmental source other than the known host eucalyptus. Trans R Soc Trop Med Hyg 1997; 91: 547–550.Google Scholar
  20. 20.
    Hill FI, Woodgyer AJ, Lintott MA. Cryptococcosis in a North Island brown kiwi (Apteryx australis mantelli) in New Zealand. J Vet Med Mycol 1995; 33: 305–309.Google Scholar
  21. 21.
    Currie B. Cryptococcus neoformans var. gattii. Lancet 1990; 8: 1442.Google Scholar
  22. 22.
    Laurenson IF, Lalloo DG, Naraqi S, et al. Cryptococcus neoformans in Papua Guinea: A common pathogen but an elusive source. J Mycol Méd 1997; 35: 437–440.Google Scholar
  23. 23.
    Swinne D, Taelman H, Batunwanayo J, Bigfiranka A, Bogaerts J. Ecology of Cryptococcus neoformans in central Africa. Med Trop; (Mars) 1994; 54: 53–55.Google Scholar
  24. 24.
    Colom Valiente MF, Alberdi M, Meseguer I, Torres-Rodriguez JM. Isolation of Cryptococcus neoformans from environmental samples in Alicante. Revista Iberoamericana de Micologia 1997; 14: 63–64.Google Scholar
  25. 25.
    Lazera MS, Cavalcanti MA, Trilles L, Nishikawa MM, Wanke B. Cryptococcus neoformans var. gattii evidence for a natural host related to decaying wood in a pottery tree hollow. Med Mycol 1998; 36: 119–122.Google Scholar
  26. 26.
    Lazera MS, Salmito Cavalcanti MA, Londero AT, Trilles L, Nishikawa MM, Wanke B. Possible primary ecological niche of Cryptococcus neoformans. Med Mycol 2000; 38: 379–383.Google Scholar
  27. 27.
    Callejas A, Odonez N, Rodriguez MC, Castaneda E. First isolation of Cryptococcus neoformans var. gattii, serotype C from the environment in Colombia. Med Myc 1998; 36: 341–344.Google Scholar
  28. 28.
    Moggi G. Inventario delle specie del genere Eucalyptus esistenti in Italia. Pubblicazioni del Centro Sperim. Agricola e Forestale 1957; 1: 1–53.Google Scholar
  29. 29.
    Criseo G, Messina S, Chindemi G. Habitat naturali di Cryptococcus neoformans nei centri urbani di Messina e Reggio Calabria: studio sulla presenza delle varietà ‘neoformans’ e ‘gattii’. Atti 1° Congr. Naz. FIMUA, Firenze, 26-27 Novembre 1992, 155–158.Google Scholar
  30. 30.
    Staib F, Seeliger HPR. Un noveau milieu sélectif pour l'isolement de Cryptococcus neoformans des matéries fécales et du sol. Ann Inst Pasteur 1996; 110: 792–793.Google Scholar
  31. 31.
    Barnett JA, Payne RW, Yarrow D. Yeast: Characteristics and Identification. 2nd ed. Cambridge: Cambridge University Press, 1990.Google Scholar
  32. 32.
    Dufait R, Velho R, De Vroey C. Rapid identification of the two varieties of Cryptococcus neoformans by Dproline assimilation. Mykosen 1987; 30: 483.Google Scholar
  33. 33.
    Pini G, Faggi E, Campisi E, Gargani G. Typing of Cryptococcus neoformans strains by Random PCR. J Mycol Méd 1998; 8: 7–12.Google Scholar

Copyright information

© Kluwer Academic Publishers 2003

Authors and Affiliations

  • E. Campisi
    • 1
  • F. Mancianti
    • 2
  • G. Pini
    • 1
  • E. Faggi
    • 1
  • G. Gargani
    • 1
  1. 1.Department of Public Health, Microbiology UnitUniversity of FlorenceFlorenceItaly
  2. 2.Department of Animal PathologyUniversity of PisaPisaItaly

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