Activated charcoal: a versatile decolorization agent for the recovery and purification of alkaline protease

  • C. Ganesh Pradeep Kumar


The use of activated charcoal for enzyme recovery and purification was investigated and the optimum activated charcoal concentration and the minimum contact time needed for efficient decolorization of an alkaline protease preparation in terms of surface adsorption and retention of enzyme activity were found to be 7.5 g l−1 and 30 min, respectively. Elevated temperatures had a greater influence on the rate of decolorization which was faster when the protease was refluxed at 60 °C for 10–15 min. These data suggest that the efficient adsorption characteristics of activated charcoal can be exploited for cost-effective downstream processing of alkaline proteases and possibly other enzymes.

Activated charcoal alkaline protease decolorization downstream processing purification 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Baker, F.S., Miller, C.E., Repik, A.J. & Tolles, E.D. 1997 Activated carbons. In Encyclopedia of Separation Technology, vol. 1, ed. Ruthven, D.M. pp. 72–93. New York: John Wiley and Sons, ISBN 0-4711-6124-1.Google Scholar
  2. Cooney, D.O. 1995 Methods for treating poisoning and drug overdose. In Activated Charcoal in Medical Applications, ed. Cooney, D.O. pp. 110–149. New York: Marcel Dekker, ISBN 0-8247-9300-5.Google Scholar
  3. Couteau, D. & Mathaly, P. 1998 Fixed-bed purification of ferulic acid from sugar-beet pulp using activated carbon: optimization studies. Bioresource Technology 64, 17–25.Google Scholar
  4. Galvano, F., Pietri, A., Bertuzzi, T., Fusconi, G., Galvano, M., Piva, A. & Piva, G. 1996 Reduction of carryover of aflatoxin from cow feed to milk by addition of activated carbon. Journal of Food Protection 59, 551–554.Google Scholar
  5. Hu, J.Y., Aizawa, T., Ookubo, Y., Morita, T. & Magara, Y. 1998 Adsorptive characteristics of ionogenic aromatic pesticides in water on powdered activated carbon. Water Research 32, 2593–2600.Google Scholar
  6. Kumar, C.G. 2002 Purification and characterization of a thermostable alkaline protease from alkalophilic Bacillus pumilus. Letters in Applied Microbiology 34, 13–17.Google Scholar
  7. Kumar, C.G. & Takagi, H. 1999 Microbial alkaline proteases: from a bioindustrial viewpoint. Biotechnology Advances 17, 561–594.Google Scholar
  8. Kumar, C.G., Malik, R.K., Tiwari, M.P. & Jany, K.D. 1999a Optimal production of Bacillus alkaline protease using a cheese whey medium. Microbiologie, Aliments and Nutrition 17, 39–48.Google Scholar
  9. Kumar, C.G., Tiwari, M.P. & Jany, K.D. 1999b Novel alkaline serine proteases from alkalophilic Bacillus sp.: purification and characterization. Process Biochemistry 34, 441–449.Google Scholar
  10. Mantell, C.L. 1942 Activated carbons and blacks. In Roger's Industrial Chemistry. 6th edn. vol. I, Chapter 18, ed. Furnas, C.C. pp. 701–735. New York: Van Nostrand.Google Scholar
  11. Sadana, A. & Beelaram, A.M. 1994 Efficiency and economics of bioseparation: some case studies. Bioseparation 4, 221–235.Google Scholar
  12. Ye, L., Khandan, N.N. & Edwards, F.G. 1994 Biological treatment of airstreams contaminated with organic vapours. Water Science and Technology 30, 71–74.Google Scholar

Copyright information

© Kluwer Academic Publishers 2003

Authors and Affiliations

  • C. Ganesh Pradeep Kumar
    • 1
    • 2
    • 1
  1. 1.Department of BiochemistryCollege of Medicine, Inha UniversityaKorea
  2. 2.Department of BiochemistryBoseCalcuttaIndia; Tel.:

Personalised recommendations