Simultaneous synthesis of enantiomerically pure (R)-1-phenylethanol and (R)-α-methylbenzylamine from racemic α-methylbenzylamine using ω-transaminase/alcohol dehydrogenase/glucose dehydrogenase coupling reaction
Article
Abstract
A simultaneous synthesis of (R)-1-phenylethanol and (R)-α-methylbenzylamine from racemic α-methylbenzyl- amine was achievied using an ω-transaminase, alcohol dehydrogenase, and glucose dehydrogenase in a coupled reaction. Racemic α-methylbenzylamine (100 mM) was converted to 49 mM (R)-1-phenylethanol (> 99% ee) and 48 mM (R)-α-methylbenzylamine (> 98% ee) in 18 h at 37 °C. This method was also used to overcome product inhibition of ω-TA by the ketone product in the kinetic resolution of racemic amines at high concentration.
alcohol dehydrogenase glucose dehydrogenase kinetic resolution NADPH regeneration ω-transaminase
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