Journal of Computer-Assisted Microscopy

, Volume 10, Issue 3, pp 123–135

Three-Dimensional Thinning Algorithm that Peels the Outmost Layer with Application to Neuron Tracing

  • Xingbai He
  • Eric Kischell
  • Marc Rioult
  • Timothy J. Holmes
Article

DOI: 10.1023/A:1023437403657

Cite this article as:
He, X., Kischell, E., Rioult, M. et al. Journal of Computer-Assisted Microscopy (1998) 10: 123. doi:10.1023/A:1023437403657

Abstract

A novel thinning algorithm for three-dimensional (3D) binary images is presented, with applications in the study of neuronal micro-anatomy by light microscopy. This algorithm satisfies properties important to many biological applications, including (a) connectivity preservation, (b) thinness, and (c) geometry preservation. It is fast to execute (a few minutes for typical 3D data sizes) on a personal computer. The algorithm addresses many challenges that are presented by 3D data. Algorithm improvements, over precursory algorithms, include the following: (1) Stricter and more exhaustive constraints on identifying outmost-layer border points are applied. (2) Border points are deleted by a novel ascending order of weighted neighbor count approach. The algorithm is robust in that it retains the above three properties (a–c) in the presence of relatively severe noise, uneven dye uptake, and nonuniform background.

Three-dimensional thinning neuron tracing skeleton light microscopy 

Copyright information

© Plenum Publishing Corporation 1998

Authors and Affiliations

  • Xingbai He
  • Eric Kischell
  • Marc Rioult
  • Timothy J. Holmes

There are no affiliations available

Personalised recommendations