Antonie van Leeuwenhoek

, Volume 83, Issue 2, pp 167–174 | Cite as

Cryptococcus haglerorum, sp. nov., an anamorphic basidiomycetous yeast isolated from nests of the leaf-cutting ant Atta sexdens

  • Wouter J. Middelhoven
  • Alvaro Fonseca
  • Solange Cristina Carreiro
  • Fernando Carlos Pagnocca
  • Odair Correa Bueno


A yeast strain (CBS 8902) was isolated from the nest of a leaf-cutting ant and was shown to be related to Cryptococcus humicola. Sequencing of the D1/D2 region of the 26S ribosomal DNA and physiological characterization revealed a separate taxonomic position. A novel species named Cryptococcus haglerorum is proposed to accommodate strain CBS 8902 that assimilates n-hexadecane and several benzene compounds. Physiological characteristics distinguishing the novel species from some other members of the C. humicola complex are presented. The phylogenetic relationship of these strains to species of the genus Trichosporon Behrend is discussed.

Atta sexdens Benzene compounds Cryptococcus haglerorum Hexadecane Leaf-cutting ants 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Barnett J.A., Payne R.W. and Yarrow D. 1983. Yeasts, Characteristics and Identification. 1st edn. Cambridge University Press, p. 605.Google Scholar
  2. Barnett J.A., Payne R.W. and Yarrow D. 2000. Yeasts, Characteristics and Identification. 3rd edn. Cambridge University Press.Google Scholar
  3. Bononi V.L.R., Autuori M. and Rocha M.B. 1981. Leucocoprinus gongylophorus (Möller) Heim, o fungo do fumigueiro de Atta sexdens rubropilosa Forel. Rickia 9: 93–97.Google Scholar
  4. Carreiro S.C., Pagnocca F.C., Bueno O.C., Bacci J., Hebling M.J.A. and da Silva O.A. 1997. Yeasts associated with nests of the leaf-cutting ant Atta sexdens rubropilosa Forel, 1908. Antonie van Leeuwenhoek 71: 243–248.PubMedCrossRefGoogle Scholar
  5. Fell J.W. and Statzell-Tallman A. 1998. Cryptococcus Vuillemin. In: Kurtzman C.P. and Fell J.W. (eds), The Yeasts–a Taxonomic Study. 4th edn. Elsevier, Amsterdam, pp. 742–767.Google Scholar
  6. Fell J.W., Boekhout T., Fonseca A., Scorzetti G. and Statzell-Tallman A. 2000. Biodiversity and systematics of basidiomycetous yeasts as determined by large-subunit rDNA D1/D2 domain sequence analysis. Int. J. Syst. Evolut. Microbiol. 50: 1351–1371.Google Scholar
  7. Gadanho M., Sampaio J.P. and Spencer-Martins I. 2001. Polyphasic taxonomy of the basidiomycetous yeast genus Rhodosporidium: R. azoricumsp.nov. Can. J. Microbiol. 46: 7–27.Google Scholar
  8. Kurtzman C.P. and Fell J.W. (eds) 1998. The Yeasts–a Taxonomic Study. 4th edn. Elsevier, Amsterdam.Google Scholar
  9. Markovetz A.J. and Kallio R.E. 1964. Assimilation of alkanes and alkenes by yeasts. J. Bacteriol. 87: 968–969.PubMedGoogle Scholar
  10. Middelhoven W.J. 1993. Catabolism of benzene compounds by ascomycetous and basidiomycetous yeasts and yeastlike fungi. A literature review and an experimental approach. Antonie van Leeuwenhoek 63: 125–144.PubMedCrossRefGoogle Scholar
  11. Middelhoven W.J. 1997. Assimilation of organic acids, the pH as determining factor. YEAST, a Newsletter for persons interested in yeast 46: 19–20.Google Scholar
  12. Middelhoven W.J. 2001. Assimilation of n-hexadecane. YEAST, a Newsletter for persons interested in yeast 50: 64–65.Google Scholar
  13. Middelhoven W.J., De Jong I.M. and De Winter M. 1991. Arxula adeninivorans, a yeast assimilating many nitrogenous and aromatic compounds. Antonie van Leeuwenhoek 59: 129–137.PubMedCrossRefGoogle Scholar
  14. Middelhoven W.J., Scorzetti G. and Fell J.W. 2000. Trichosporon veenhuisii sp. nov., an alkane-assimilating anamorphic basidiomycetous yeast. Int. J. Syst. Evol. Microbiol. 50: 381–387.PubMedGoogle Scholar
  15. Middelhoven W.J., Scorzetti G. and Fell J.W. 2001. Trichosporon porosum comb.nov., an anamorphic basidiomycetous yeast inhabiting soil, related to the loubieri /laibachii group of species that assimilate hemicelluloses and phenolic compounds. FEMS Yeast Research 1: 1–8.Google Scholar
  16. Möller A. 1893. Die Pilzgärten einiger südamerikanischen Ameisen. Botanische Mitteilung aus den Tropen 6: 1–127.Google Scholar
  17. Pagnocca F.C., Baccci M. Jr, Fungaro M.H., Bueno O.C., Hebling M.J., Sant'Anna A. et al. 2001. RAPD analysis of the sexual state and sterile mycelium of the fungus cultivated by the leaf-cutting ant Acromyrmex hispidus fallax. Mycological Research 105: 173–176.CrossRefGoogle Scholar
  18. Sampaio J.P. 1999. Utilization of low molecular weight aromatic compounds by hetero-basidiomycetous yeasts: taxonomic implications. Can. J. Microbiol. 45: 491–512.PubMedCrossRefGoogle Scholar
  19. Singer R. 1986. The Agaricales in Modern Taxonomy. 4th edn. Koeltz Scientific Books, Königstein, Germany.Google Scholar
  20. Sugita T., Takashima M., Ikeda R., Nakase T. and Shinoda T. 2000. Phylogenetic and taxonomic heterogeneity of Cryptococcus humicolus by analysis of the sequences of the internal transcribed spacer regions and 18S rDNA, and the phylogenetic relationships of C. humicolus, C. curvatus and the genus Trichosporon. Microbiol. Immunol. 44: 455–461.PubMedGoogle Scholar
  21. Sugita T., Takashima M., Nakase T., Ichikawa T., Ikeda R. and Shinoda T. 2001. Two new yeasts, Trichosporon debeurmannianum sp. nov. and Trichosporon dermatis sp. nov., transferred from the Cryptococcus humicola complex. Int. J. Syst. Evolut. Microbiol. 51: 1221–1228.Google Scholar
  22. Takashima M., Sugita T., Shinoda T. and Nakase T. 2001. Reclassification of the Cryptococcus humicola complex. Int. J. Syst. Evolut. Microbiol. 51: 2199–2210.Google Scholar
  23. Yarrow D. 1998. Methods for the isolation, maintenance and identification of yeasts. In: Kurtzman C.P. and Fell J.W. (eds), The Yeasts–a Taxonomic Study. 4th edn. Elsevier, Amsterdam, pp. 77–100.Google Scholar

Copyright information

© Kluwer Academic Publishers 2003

Authors and Affiliations

  • Wouter J. Middelhoven
    • 1
  • Alvaro Fonseca
    • 2
  • Solange Cristina Carreiro
    • 3
  • Fernando Carlos Pagnocca
    • 3
  • Odair Correa Bueno
    • 3
  1. 1.Laboratorium voor MicrobiologieWageningen UniversityWageningenThe Netherlands
  2. 2.Centro de Recursos Microbiológicos, Facultade de Ciencias e TecnologiaUniversidade Nova de LisboaCaparicaPortugal
  3. 3.Centro de Estudos de Insetos SociaisUniversidade Estadual Paulista _ UNESPRio ClaroBrazil

Personalised recommendations