The Histochemical Journal

, Volume 34, Issue 6–7, pp 305–312 | Cite as

Small Heat Shock Proteins Expression in Rat Kidneys Treated with Cyclosporine A Alone and Combined with Melatonin

  • Alessandra Stacchiotti
  • Rita Rezzani
  • Paola Angoscini
  • Luigi Rodella
  • Rossella Bianchi


Small heat shock proteins (sHSPs) are cytoskeletal chaperones constitutively expressed in the normal kidney but enhanced with beneficial roles during adverse stimuli. Cyclosporine A is an immunosuppressive drug with major adverse side effect such as severe nephrotoxicity. Among possible mechanisms of cyclosporine A-induced renal damage, oxidative stress and cytoskeletal damage have been suggested. Melatonin has been successfully used as antioxidant against many renal diseases. This in vivo study was performed to shed light on the protective effect of melatonin against cyclosporine A-induced renal alterations. We treated rats with cyclosporine A alone, or combined with melatonin, and with melatonin alone (as controls) for 40 days and analysed the renal abundance and distribution of two sHSPs, HSP25 and alpha B-crystallin. These data were correlated with the histopathological effects of the treatments. Cyclosporine A induced insoluble isoforms that moved to soluble fractions after melatonin coadministration as in controls. After cyclosporine A treatment, an intense signal for sHSPs was found within the glomeruli, nucleus and cytoplasm of cortical tubules, collecting ducts and vascular wall. After melatonin supply, the staining was faint, limited to the cytoplasm of cortical tubules, similar to controls. Both fibrosis and tubular alterations significantly decreased after melatonin coadministration. In conclusion, HSP25 and alpha B-crystallin are overexpressed in the rat kidney treated with cyclosporine A but are similar to controls after combined melatonin. This could be a consequence of the cytoprotective effect of melatonin in this nephrotoxic model so that a beneficial sHSPs response isbreak unnecessary.


Cyclosporine Melatonin Proximal Tubule Distal Tubule Small Heat Shock Protein 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© Kluwer Academic Publishers 2002

Authors and Affiliations

  • Alessandra Stacchiotti
    • 1
  • Rita Rezzani
    • 1
  • Paola Angoscini
    • 1
  • Luigi Rodella
    • 1
  • Rossella Bianchi
    • 1
  1. 1.Division of Human Anatomy, Department of Biomedical Sciences and BiotechnologyUniversity of BresciaBresciaItaly

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