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Russian Journal of Genetics

, Volume 39, Issue 4, pp 395–399 | Cite as

Suppression of Nonsense and Frameshift Mutations Obtained by Different Methods for Inactivating the Translation Termination Factor eRF3 in Yeast Saccharomyces cerevisiae

  • S. P. Zadorsky
  • A. S. Borchsenius
  • Yu. V. Sopova
  • V. A. Starzev
  • S. G. Inge-Vechtomov
Article

Abstract

Mutations in genes of omnipotent nonsense suppressors SUP35 and SUP45 in yeast Saccharomyces cerevisiae encoding translation termination factors eRF3 and eRF1, respectively, and prionization of the eRF3 protein may lead to the suppression of some frameshift mutations (CPC mutations). Partial inactivation of the translation termination factor eRF3 was studied in strains with unstable genetically modified prions and also in transgenic yeast S. cerevisiae strains with the substitution of the indigenous SUP35 gene for its homolog from Pichia methanolica or for a recombinant S. cerevisiae SUP35gene. It was shown that this partial inactivation leads not only to nonsense suppression, but also to suppression of the frameshift lys2-90 mutation. Possible reasons for the correlation between nonsense suppression and suppression of the CPC lys2-90 mutation and mechanisms responsible for the suppression of CPC mutations during inactivation of translation termination factors are discussed.

Keywords

Saccharomyces Cerevisiae Frameshift Mutation Termination Factor Translation Termination Partial Inactivation 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© MAIK “Nauka/Interperiodica” 2003

Authors and Affiliations

  • S. P. Zadorsky
    • 1
  • A. S. Borchsenius
    • 1
  • Yu. V. Sopova
    • 1
  • V. A. Starzev
    • 1
  • S. G. Inge-Vechtomov
    • 1
  1. 1.Department of Genetics and BreedingSt. Petersburg State UniversitySt. PetersburgRussia

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