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Trypsin-Banding Induced Volume Changes of Human Metaphase Chromosomes Analyzed by Scanning Force Microscopy

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Journal of Computer-Assisted Microscopy

Abstract

A procedure for volume estimation based on scanning force microscopy images is applied to the study of banding-induced structural changes of chromosomes. Therefore, metaphase chromosomes were imaged before and after trypsin digestion, and the resulting three-dimensional data sets were used for a determination of the volumes of the imaged structures. The procedure is based on a histogram-based thresholding. The estimated volume is corrected for the background signal using the average background value from the histogram, so that an automated analysis of the images is possible. A first set of experimental data processed according to this approach is presented.

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Authors and Affiliations

  1. Institute of Physical High Technology, Jena, Germany

    W. Fritzsche & R. Augustin

  2. Institute of Human Genetics and Anthropology, Friedrich-Schiller University, Jena, Germany

    S. Michel & U. Claussen

Authors
  1. W. Fritzsche
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  2. R. Augustin
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  3. S. Michel
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  4. U. Claussen
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Correspondence to W. Fritzsche.

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Fritzsche, W., Augustin, R., Michel, S. et al. Trypsin-Banding Induced Volume Changes of Human Metaphase Chromosomes Analyzed by Scanning Force Microscopy. Journal of Computer-Assisted Microscopy 10, 71–76 (1998). https://doi.org/10.1023/A:1023312421502

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  • DOI: https://doi.org/10.1023/A:1023312421502

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