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Expression of Nuclear Receptors and Apo E Secretion During the Differentiation of Monocytic THP-1 cells into Macrophages

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References

  • Auboeuf D, Rieusset J, Fajas L, et al. Tissue distribution and quantification of the expression of mRNAs of peroxisome proliferator-activated receptors and liver X receptor-α in humans: no alteration in adipose tissue of obese and NIDDM patients. Diabetes. 1997;46(8):1319-27.

    PubMed  CAS  Google Scholar 

  • Auwerx J. The human leukemia cell line, THP-1: a multi-facetted model for the study of monocyte-macrophage differentiation. Experientia. 1991;47(1):22-31.

    Article  PubMed  CAS  Google Scholar 

  • Auwerx JH, Deeb S, Brunzell JD, Peng R, Chait A. Transcriptional activation of the lipoprotein lipase and apolipoprotein E genes accompanies differentiation in some human macrophage-like cell lines. Biochemistry. 1988;27(8):2651-5.

    Article  PubMed  CAS  Google Scholar 

  • Basu SK, Ho YK, Brown MS, Bilheimer DW, Anderson RG, Goldstein JL. Biochemical and genetic studies of the apoprotein E secreted by mouse macrophages and human monocytes. J Biol Chem. 1982;257(16):9788-95.

    PubMed  CAS  Google Scholar 

  • Brown G, Bunce CM, Rowlands DC, Williams GR. All-trans retinoic acid and 1 α,25-dihydroxyvitamin D3 co-operate to promote differentiation of the human promyeloid leukemia cell line HL60 to monocytes. Leukemia. 1994;8(5):806-15.

    PubMed  CAS  Google Scholar 

  • Brown MS, Goldstein JL. Lipoprotein receptors in the liver. Control signals for plasma cholesterol traffic. J Clin Invest. 1983; 72(3):743-7.

    PubMed  CAS  Google Scholar 

  • Chawla A, Saez E, Evans RM. “Don't know much bile-ology. Cell. 2000; 103(1):1-4.

    Article  PubMed  CAS  Google Scholar 

  • Chawla A, Boisvert WA, Lee CH, et al. A PPARγ-LXR-ABCA1 pathway in macrophages is involved in cholesterol efflux and atherogenesis. Mol Cell. 2001;7(1):161-71.

    Article  PubMed  CAS  Google Scholar 

  • Chinetti G, Griglio S, Antonucci M, et al. Activation of proliferator-activated receptors α and γ induces apoptosis of human monocyte-derived macrophages. J Biol Chem. 1998;273(40):25573-80.

    Article  PubMed  CAS  Google Scholar 

  • Chinetti G, Lestavel S, Bocher V, et al. PPAR-α and PPAR-γ activators induce cholesterol removal from human macrophage foam cells through stimulation of the ABCA1 pathway. Nat Med. 2001;7(1):53-8.

    Article  PubMed  CAS  Google Scholar 

  • Choudhury A, Singh RK, Moniaux N, El Metwally TH, Aubert JP, Batra SK. Retinoic acid-dependent transforming growth factor-β2-mediated induction of MUC4 mucin expression in human pancreatic tumor cells follows retinoic acid receptor-α signaling pathway. J Biol Chem. 2000; 275(43):33929-36.

    Article  PubMed  CAS  Google Scholar 

  • Cutolo M, Villaggio B, Bisso A, Sulli A, Coviello D, Dayer JM. Presence of estrogen receptors in human myeloid monocytic cells (THP-1 cell line). Eur Cytokine Netw. 2001;12(2):368.

    PubMed  CAS  Google Scholar 

  • Defacque H, Sevilla C, Piquemal D, Rochette-Egly C, Marti J, Commes T. Potentiation of VD-induced monocytic leukemia cell differentiation by retinoids involves both RAR and RXR signaling pathways. Leukemia. 1997;11(2):221-7.

    Article  PubMed  CAS  Google Scholar 

  • Dory L. Regulation of apolipoprotein E secretion by high density lipoprotein 3 in mouse macrophages. J Lipid Res. 1991;32(5):783-92.

    PubMed  CAS  Google Scholar 

  • Edwards PA, Kast HR, Anisfeld AM. BAREing it all. The adoption of lxr and fxr and their roles in lipid homeostasis. J Lipid Res. 2002;43(1):2-12.

    PubMed  CAS  Google Scholar 

  • Galetto R, Albajar M, Polanco JI, Zakin MM, Rodriguez-Rey JC. Identification of a peroxisome-proliferator-activated-receptor response element in the apolipoprotein E gene control region. Biochem J. 2001;357(Pt 2):521-7.

    Article  PubMed  CAS  Google Scholar 

  • Gardiner EM, Baldock PA, Thomas GP, et al. Increased formation and decreased resorption of bone in mice with elevated vitamin D receptor in mature cells of the osteoblastic lineage. FASEB J. 2000;14(13):1908-16.

    Article  PubMed  CAS  Google Scholar 

  • Giguere V. Orphan nuclear receptors: from gene to function. Endocr Rev. 1999;20(5):689-725.

    Article  PubMed  CAS  Google Scholar 

  • Hanley K, Komuves LG, Ng DC, et al. Farnesol stimulates differentiation in epidermal keratinocytes via PPARα. J Biol Chem. 2000;275(15):11484-91.

    Article  PubMed  CAS  Google Scholar 

  • Heyman RA, Mangelsdorf DJ, Dyck JA, et al. 9-cis Retinoic acid is a high affinity ligand for the retinoid X receptor. Cell. 1992;68(2):397-406.

    Article  PubMed  CAS  Google Scholar 

  • Janowski BA, Willy PJ, Devi TR, Falck JR, Mangelsdorf DJ. An oxysterol signalling pathway mediated by the nuclear receptor LXRα. Nature. 1996;383(6602):728-31.

    Article  PubMed  CAS  Google Scholar 

  • Jansen J, Uitdehaag B, Koper JW, van Den Berg TK. Glucocorticoid receptor ligand binding in monocytic cells using a microplate assay. Pathobiology. 1999;67(5–6):262-4.

    Article  PubMed  CAS  Google Scholar 

  • Kreutz M, Fritsche J, Ackermann U, Krause SW, Andreesen R. Retinoic acid inhibits monocyte to macrophage survival and differentiation. Blood. 1998;91(12):4796-802.

    PubMed  CAS  Google Scholar 

  • Laffitte BA, Repa JJ, Joseph SB, et al. LXRs control lipid-inducible expression of the apolipoprotein E gene in macrophages and adipocytes. Proc Natl Acad Sci USA. 2001;98(2):507-12.

    Article  PubMed  CAS  Google Scholar 

  • Larkin L, Khachigian LM, Jessup W. Regulation of apolipoprotein E production in macrophages (review). Int J Mol Med. 2000;6(3):253-8.

    PubMed  CAS  Google Scholar 

  • Lehmann JM, Moore LB, Smith-Oliver TA, Wilkison WO, Willson TM, Kliewer SA. An antidiabetic thiazolidinedione is a high affinity ligand for peroxisome proliferator-activated receptor γ (PPARγ). J Biol Chem. 1995;270(22):12953-6.

    Article  PubMed  CAS  Google Scholar 

  • Lehmann JM, McKee DD, Watson MA, Willson TM, Moore JT, Kliewer SA. The human orphan nuclear receptor PXR is activated by compounds that regulate CYP3A4 gene expression and cause drug interactions. J Clin Invest. 1998;102(5):1016-23.

    Article  PubMed  CAS  Google Scholar 

  • Levin AA, Sturzenbecker LJ, Kazmer S, et al. 9-cis Retinoic acid stereoisomer binds and activates the nuclear receptor RXRα. Nature. 1992;355(6358):359-61.

    Article  PubMed  CAS  Google Scholar 

  • Linton MF, Fazio S. Macrophages, lipoprotein metabolism, and atherosclerosis: insights from murine bone marrow transplantation studies. Curr Opin Lipidol. 1999;10(2):97-105.

    Article  PubMed  CAS  Google Scholar 

  • Lu TT, Repa JJ, Mangelsdorf DJ. Orphan nuclear receptors as eLiXiRs and FiXeRs of sterol metabolism. J Biol Chem. 2001;276(41):37735-8.

    PubMed  CAS  Google Scholar 

  • Mahley RW. Apolipoprotein E: cholesterol transport protein with expanding role in cell biology. Science. 1988;240(4852):622-30.

    PubMed  CAS  Google Scholar 

  • Mangelsdorf DJ, Koeffler HP, Donaldson CA, Pike JW, Haussler MR. 1,25-Dihydroxyvitamin D3-induced differentiation in a human promyelocytic leukemia cell line (HL-60): receptor-mediated maturation to macrophage-like cells. J Cell Biol. 1984;98(2):391-8.

    Article  PubMed  CAS  Google Scholar 

  • Mangelsdorf DJ, Thummel C, Beato M, et al. The nuclear receptor superfamily: the second decade. Cell. 1995;83(6):835-9.

    Article  PubMed  CAS  Google Scholar 

  • Mazzone T. Apolipoprotein E secretion by macrophages: its potential physiological functions. Curr Opin Lipidol. 1996;7(5):303-7.

    Article  PubMed  CAS  Google Scholar 

  • Menju M, Tajima S, Yamamoto A. Expression of the apolipoprotein E gene in a human macrophage-like cell line, THP-1. J Biochem (Tokyo). 1989;106(3):505-10.

    CAS  Google Scholar 

  • Oliver WR Jr, Shenk JL, Snaith MR, et al. A selective peroxisome proliferator-activated receptor delta agonist promotes reverse cholesterol transport. Proc Natl Acad Sci USA. 2001;98(9):5306-11.

    Article  PubMed  CAS  Google Scholar 

  • Rees D, Sloane T, Jessup W, Dean RT, Kritharides L. Apolipoprotein A-I stimulates secretion of apolipoprotein E by foam cell macrophages. J Biol Chem. 1999;274(39):27925-33.

    Article  PubMed  CAS  Google Scholar 

  • Schultz JR, Tu H, Luk A, et al. Role of LXRs in control of lipogenesis. Genes Dev. 2000;14(22):2831-8.

    Article  PubMed  CAS  Google Scholar 

  • Simpson DA, Feeney S, Boyle C, Stitt AW. Retinal VEGF mRNA measured by SYBR green I fluorescence: a versatile approach to quantitative PCR. Mol Vis. 2000;6(8):178-83.

    PubMed  CAS  Google Scholar 

  • Staels B, Koenig W, Habib A, et al. Activation of human aortic smooth-muscle cells is inhibited by PPARα but not by PPARγ activators. Nature. 1998;393(6687):790-3.

    Article  PubMed  CAS  Google Scholar 

  • Tontonoz P, Nagy L, Alvarez JG, Thomazy VA, Evans RM. PPARgamma promotes monocyte/macrophage differentiation and uptake of oxidized LDL. Cell. 1998;93(2):241-52.

    Article  PubMed  CAS  Google Scholar 

  • Tsuchiya S, Yamabe M, Yamaguchi Y, Kobayashi Y, Konno T, Tada K. Establishment and characterization of a human acute monocytic leukemia cell line (THP-1). Int J Cancer. 1980;26(2):171-6.

    PubMed  CAS  Google Scholar 

  • Tsuchiya S, Kobayashi Y, Goto Y, et al. Induction of maturation in cultured human monocytic leukemia cells by a phorbol diester. Cancer Res. 1982;42(4):1530-6.

    PubMed  CAS  Google Scholar 

  • Venkateswaran A, Repa JJ, Lobaccaro JM, Bronson A, Mangelsdorf DJ, Edwards PA. Human white/murine ABC8 mRNA levels are highly induced in lipid-loaded macrophages. A transcriptional role for specific oxysterols. J Biol Chem. 2000;275(19):14700-7.

    Article  PubMed  CAS  Google Scholar 

  • von Eckardstein A, Nofer JR, Assmann G. High density lipoproteins and arteriosclerosis. Role of cholesterol efflux and reverse cholesterol transport. Arterioscler Thromb Vasc Biol. 2001;21(1):13-27.

    PubMed  CAS  Google Scholar 

  • Vosper H, Patel L, Graham TL, et al. The peroxisome proliferator-activated receptor delta promotes lipid accumulation in human macrophages. J Biol Chem. 2001;276(47):44258-65.

    Article  PubMed  CAS  Google Scholar 

  • Werb Z, Chin JR. Onset of apoprotein E secretion during differentiation of mouse bone marrow-derived mononuclear phagocytes. J Cell Biol. 1983;97(4):1113-18.

    Article  PubMed  CAS  Google Scholar 

  • Zhu L, Gong B, Bisgaier CL, Aviram M, Newton RS. Induction of PPARγ1 expression in human THP-1 monocytic leukemia cells by 9-cis-retinoic acid is associated with cellular growth suppression. Biochem Biophys Res Commun. 1998;251(3):842-8.

    Article  PubMed  CAS  Google Scholar 

  • Zimber A, Chedeville A, Abita JP, Barbu V, Gespach C. Functional interactions between bile acids, all-trans retinoic acid, and 1,25-dihydroxy-vitamin D3 on monocytic differentiation and myeloblastin gene down-regulation in HL60 and THP-1 human leukemia cells. Cancer Res. 2000;60(3):672-8.

    PubMed  CAS  Google Scholar 

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Perez, A., Thuillard, JL., Bentzen, C. et al. Expression of Nuclear Receptors and Apo E Secretion During the Differentiation of Monocytic THP-1 cells into Macrophages. Cell Biol Toxicol 19, 95–105 (2003). https://doi.org/10.1023/A:1023307206125

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