Advertisement

Production of poly(β-hydroxybutyrate) by Comamonas testosteroni during growth on naphthalene

Abstract

Comamonas testosteroni has been found to produce poly(β-hydroxybutyrate) (PHB) during its growth on naphthalene. Fourier transform infrared spectroscopy (FTIR) and 13C nuclear magnetic resonance (NMR) analysis confirmed it as a homopolymer of 3-hydroxybutyrate. Oxygen and essential nutrient limitation other than carbon source play a major role in maximum PHB production. Nitrogen limitation was found to have a profound effect, with 0.2 g ammonium nitrate/l optimum for PHB production. Both aeration and iron were found to be essential for growth and PHB accumulation. Ferric chloride at 0.04 g/l concentration was found to be optimum for PHB accumulation. Phosphate source variation showed no significant effect. Using naphthalene as a sole carbon source in optimized Bushnell Haas medium, 85% of the dry cell mass was extracted as chloroform-soluble PHB.

This is a preview of subscription content, log in to check access.

References

  1. Anderson, A.J. & Dawes, E.A. 1990 Occurrence, metabolism, metabolic role and industrial uses of bacterial polymeric-hydroxy alkanoates. Microbiological Reviews 54, 450-472.

  2. Asano, Y. & Watanabe, S. 2001 Isolation of poly (3-hydroxybutyrate) (PHB)-degrading microorganisms and characterisation of PHB-depolymerase from Arthrobacter sp. strain W6. Bioscience,Biotechnology and Biochemistry 65, 1191-1194.

  3. Beller, H.R., Grbic-Galic, D. & Reinhard M. 1992 Microbial degradation of toluene under sulphate reducing condition and the influence of iron on the process. Applied and Environmental Microbiology 58, 786-793.

  4. Choi, M.H. & Yoon, S.C. 1994 Polymer biosynthesis characteristics of Pseudomonas citronellolis grown on various carbon sources, including 3-methyl-branched substrates. Applied and Environmental Microbiology 60, 3245-3254.

  5. Dawes, E.A. 1986 Microbial energy reserve compounds, In Microbial energetics, ed. Dawes, E.A. pp. 145-165. London, United Kingdom: Blackie. ISBN 0-21691790-5.

  6. Findlay, R.H. & White, D.C. 1983 Polymeric beta-hydroxyalkanoates from environmental samples and Bacillus megaterium. Applied and Environmental Microbiology 41, 9-16.

  7. Grund, E., Denecke, B. & Eichenlaub, R. 1992 Naphthalene degradation via salicyclate and gentisate by Rhodococcus sp. strain B4. Applied and Environmental Microbiology 58, 1874-1877.

  8. Haigler, B.E. & Gibson, D.T. 1990a Purification and properties of FerredoxinNAP, a component of naphthalene dioxygenase form Pseudomonas sp. Strain NCIB 9816. Journal of Bacteriology 172, 465-468.

  9. Haigler, B.E. & Gibson, D.T. 1990b Purification and properties of NADH-FerredoxinNAP reductase, a component of naphthalene dioxygenase from Pseudomonas sp. Strain NCIB 9816. Journal of Bacteriology 172, 457-464.

  10. Huisman, G.W., Wonink, E., Meima, R., Kazemier, B., Terpstra, P. & Witholt, B. 1991 Metabolism of poly (3-hydroxyalkanoates) (PHAs) by Pseudomonas oleovorans. The Journal of Biological Chemistry 266, 2191-2198.

  11. James, B.W., Mauchline, W.S., Dennis, P.J. & Keevil C.W. 1999 Poly-3-hydroxybutyrate in Legionella pneumophila, an energy source for survival in low-nutrient environments. Applied and Environmental Microbiology 65, 822-827.

  12. Kessler, B. & Witholt, B. 1999 Poly (3-hydroxyalkanoates). Encyclopaedia of Bioprocess Technology: Fermentation, Biocatalysis and Bioseparation. pp. 2024-2040. John Wiley & sons, Inc. ISBN 0-471-13822-3.

  13. Kim, Byung-K.I., Yoon, S.C., Jae, D.D. N. & Lenz, R.W. 1997 Effect of C/N ratio on the production of poly (3-hydroxyalkanoates) by the Methylotroph Paracoccus denitrificans. Journal of Microbiology and Biotechnology 7,391-396.

  14. Klinke, S., Ren, Q., Witholt, B. & Kessler, B. 1999 Production of medium-chain-length poly(3-hydroxyalkanoates) from gluconate by recombinant Escherichia coli. Applied and Environmental Microbiology 65, 540-548.

  15. Lageveen, R.G., Huisman, G.W., Prevsting, H., Ketelaar, P., Eggink, G. & Witholt, B. 1988 Formation of polyesters by Pseudomonas oleovorans: effect of substrates on formation and composition of poly-(R)-3-hydroxyalkanoates and poly-(R)-3-hydroxyalkenoates. Applied and Environmental Microbiology 54, 2924-2932.

  16. Lefebvre, G., Rocher, M. & Braunegg, G. 1997 Effect of low dissolvedoxygen concentrations on poly-(3-hydroxybutyrate-co-3-hydroxyvalerate) production by Alcaligenes eutrophus. Applied and Environmental Microbiology 63, 827-833.

  17. Meyer, J.M. & Abdallah, M.A. 1978 The Fluorescent pigment of Pseudomonas fluorescens: biosynthesis, purification and physiological properties. Journal of General Microbiology 107, 319-328.

  18. Mukai, K., Yamada, K. & Doi, Y. 1993 Kinetics and mechanism of heterogeneous hydrolysis of poly[(R)-hydroxybutyrate] film by PHA depolymerases. International Journal of Biological Macromolecules 15, 361-366.

  19. Slepecky, R.A. & Law, J.H. 1960 A rapid Spectrophotometric assay for alpha, beta-unsaturated acids and beta-hydroxy acids. Analytical Chemistry 32, 1697-1699.

  20. Steinbuchel, A. & Schlegel, H.G. 1991 Physiology and molecular genetics of poly(?-hydroxyalkanoic acid) synthesis in Alcaligenes eutrophus. Molecular Microbiology 5, 535-542.

  21. Steinbuchel, A. & Hein, S. 2001 Biochemical and molecular basis of microbial synthesis of polyhydroxyalkanoates in microorganisms. Advances in Biochemical Engineering/Biotechnology 71, 81-123.

  22. Steinbuchel, A. & Schlegel, H.G. 1989 Extraction of pyruvate by mutants of Alcaligenes eutrophus which are impaired in the accumulation of poly(?-hydroxybutyric acid) (PHB), under conditions permitting synthesis of PHB. Applied Microbiology and Biotechnology 31, 168-175.

  23. Yagi, K., Miyawaki, I., Kayushita, A., Kondo, M., Kitano, Y., Murakami, Y., Maeda, I., Umeda, F., Miura, Y., Kawase, M. & Mizoguchi, T. 1996 Biosynthesis of poly(3-hydroxyalkanoic acid) copolymers from CO2 in Pseudomonas acidophila through introduction of the DNA fragment responsible for chemolithoautotrophic growth of Alcaligenes hydrogenophilus. Applied and Environmental Microbiology 62, 1004-1007.

Download references

Author information

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Thakor, N., Patel, M., Trivedi, U. et al. Production of poly(β-hydroxybutyrate) by Comamonas testosteroni during growth on naphthalene. World Journal of Microbiology and Biotechnology 19, 185–189 (2003) doi:10.1023/A:1023295009846

Download citation

  • Bushnell Haas medium
  • Comamonas testosteroni
  • poly(β-hydroxybutyrate)