Comamonas testosteroni has been found to produce poly(β-hydroxybutyrate) (PHB) during its growth on naphthalene. Fourier transform infrared spectroscopy (FTIR) and 13C nuclear magnetic resonance (NMR) analysis confirmed it as a homopolymer of 3-hydroxybutyrate. Oxygen and essential nutrient limitation other than carbon source play a major role in maximum PHB production. Nitrogen limitation was found to have a profound effect, with 0.2 g ammonium nitrate/l optimum for PHB production. Both aeration and iron were found to be essential for growth and PHB accumulation. Ferric chloride at 0.04 g/l concentration was found to be optimum for PHB accumulation. Phosphate source variation showed no significant effect. Using naphthalene as a sole carbon source in optimized Bushnell Haas medium, 85% of the dry cell mass was extracted as chloroform-soluble PHB.
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Thakor, N., Patel, M., Trivedi, U. et al. Production of poly(β-hydroxybutyrate) by Comamonas testosteroni during growth on naphthalene. World Journal of Microbiology and Biotechnology 19, 185–189 (2003) doi:10.1023/A:1023295009846
- Bushnell Haas medium
- Comamonas testosteroni