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Journal of Assisted Reproduction and Genetics

, Volume 15, Issue 4, pp 188–192 | Cite as

Relationship Between Creatine Kinase Levels and Clinical Diagnosis of Infertility

  • R. S. Sidhu
  • J. Hallak
  • R. K. Sharma
  • A. J. ThomasJr.
  • A. Agarwal
Article

Abstract

Purpose: The creatine kinase level indicates sperm maturity and correlates with the spermatozoal fertilizing potential. The relationship between creatine kinase levels in subfertile men and their clinical diagnosis was examined.

Methods:Patients with unexplained infertility (n = 34), varicocele (n = 20), postvasectomy reversal (n = 7), or cancer (n = 22) were included in this prospective clinical study. The control group consisted of healthy normal donors (n = 15).

Results:The median and interquartile range values of creatine kinase for each group were as follows: normal donors, 0.061 U/108sperm (0.056 to 0.076 U/108sperm); idiopathic malefactor, 0.119 U/108sperm (0.061 to 0.190 U/108sperm); varicocele, 0.392 U/108sperm (0.209 to 1.494 U/108sperm); postvasectomy reversal, 0.589 U/108sperm (0.425 to 4.043 U/108sperm); and cancer, 0.068 U/108sperm (0.047 to 0.168 U/108sperm). Sperm creatine kinase levels were significantly higher in patients with varicocele compared to normal donors (P = 0.0001), cancer patients (P = 0.0002), and men with idiopathic infertility (P = 0.0009). Sperm concentration and creatine kinase level were inversely correlated in patients (r = −0.7,P < 0.001) but not in normal donors.

Conclusions: Semen quality is poorer in subfertile patients with clinical varicocele and postvasectomy reversal than in cancer patients and patients with idiopathic male infertility. That the creatine kinase levels in cancer patients were similar to those of normal donors suggests that the final phase of spermatogenesis may not be altered in men with cancer; thus semen from these patients should be banked to ensure fertility after cancer treatment.

cancer patients postvasectomy reversal idiopathic infertility sperm creatine kinase varicocele 

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REFERENCES

  1. 1.
    Huszar G, Vigue L: Activities of sperm creatine kinase and dynein ATPase in oligospermic men. Ann NY Acad Sci 1987;513:602–605Google Scholar
  2. 2.
    Sigman M, Lipshultz LI, Howards SS: Evaluation of subfertile male. In Infertility in the Male, LI Lipshultz, SS Howards (eds). Philadelphia, Mosby Year Book, 1991, pp. 179–210Google Scholar
  3. 3.
    Irvine DS: Assessment of spermatogenesis. Curr Obstet Gynaecol 1992;2:20–26Google Scholar
  4. 4.
    Nachtigall RD, Faure N, Glass RH: Artificial insemination with husband's sperm. Fertil Steril 1979;32:141–147Google Scholar
  5. 5.
    Aitken RJ, Irvine DS, Wu FC: Prospective analysis of spermoocyte fusion and reactive oxygen species genration as criteria for the diagnosis of infertility. Am J Obstet Gynecol 1991;164:542–551Google Scholar
  6. 6.
    Abdual-Aziz M, MacLusky NJ, Bhavani BR, Casper RF: Hyaluronidase activity in human semen: Correlation with fertilization in vitro. Fertil Sertil 1995;64:1147–1153Google Scholar
  7. 7.
    Agarwal A, Loughlin KR: Acrosin activity in patients with idiopathic infertility. Arch Androl 1991;27:97–101Google Scholar
  8. 8.
    Tombes RM, Shapiro BM: Energy transport and cell polarity: Relationship of phosphagen kinase activity to sperm function. J Exp Zool 1989;251:82–90Google Scholar
  9. 9.
    Huszar G, Vigue L, Corrales M: Sperm creatine kinase activity in fertile and infertile oligospermic men. J Androl 1990;11:40–46Google Scholar
  10. 10.
    Huszar G, Vigue L, Corrales M: Sperm creatine phosphokinase M-isoform ratios and fertilizing potential of men: A blinded study of 84 couples treated with in vitro fertilization. Fertil Steril 1992;57:882–888Google Scholar
  11. 11.
    Huszar G, Vigue L: Correlation between the rate of lipid peroxidation and cellular maturity as measured by creatine kinase activity in human spermatozoa. J Androl 1994;15:71–77Google Scholar
  12. 12.
    Huszar G, Vigue L: Incomplete development of human spermatozoa is associated with increased creatine phoshokinase concentration and abnormal head morphology. Mol Reprod Dev 1993;34:292–298Google Scholar
  13. 13.
    World Health Organization: WHO Laboratory Manual for the Examination of Human Semen and Semen-Cervical Mucus Interaction, 3rd ed. Cambridge, Cambridge University Press, 1992Google Scholar
  14. 14.
    Parinaud J, Vieitez G, Moutaffian H, Richoilley G, Labal B: Relevance of acrosome function in the evaluation of semen in vitro fertilizing ability. Fertil Steril 1995;63:598–603Google Scholar
  15. 15.
    Gomez E, Buckingham DW, Brindle J, Lanzafame F, Irvine DS, Aitken J: Development of an image analysis system to monitor the retention of residual cytoplasm by human spermatozoa: Correlation with biochemical markers of the cytoplasmic space, oxidative stress, and sperm function. J Androl 1996;17:276–287Google Scholar
  16. 16.
    Aitken RJ, Krausz C, Buckingham D: Relationships between biochemical markers for residual sperm cytoplasm, reactive oxygen species generation, and the presence of leucocytes and precursor germ cells in human sperm suspensions. Mol Reprod Dev 1994;39:268–279Google Scholar
  17. 17.
    Hammond MG, Sloan CS, Hall JL: Application of interspecies in vitro fertilization in the initial assessment of infertile couple. Am J Obstet Gynecol 1982;142:340–343Google Scholar
  18. 18.
    Peckham MJ, McElwain TJ, Barrett A: Hodgkin's disease. In Treatment of Cancer, KE Halnan, K Sikora (eds). London, Chapman & Hall, 1990, pp. 691–713Google Scholar
  19. 19.
    Chapman RM, Sutcliffe SB, Malpas JS: Male gonadal dysfunction in Hodgkin's disease. A prospective study. JAMA 1981;245:1323–1328Google Scholar
  20. 20.
    Sanger WG, Olson JH, Sherman JK: Semen cryobanking for men with cancer-criteria change. Fertil Steril 1992;58:1024–1027Google Scholar
  21. 21.
    Davis OK, Bedford JM, Berkeley AS, Graf MJ, Rosenwaks Z: Pregnancy achieved through in vitro fertilization with cryopreserved semen from a man with Hodgkin's lymphoma. Fertil Steril 1990;53:377–378Google Scholar
  22. 22.
    Khalifa E, Oehninger S, Acosta AA, Morshedi M, Veeck L, Bryzyski RG, Mausher SJ: Successful fertilization and pregnancy outcome in in-vitro fertilization using cryopreserved/ thawed spermatozoa from patients with malignant diseases. Hum Reprod 1992;7:105–108Google Scholar
  23. 23.
    Scammell GE, White N, Stedrnoska J, Hendry WF. Edmonds DK, Jeffcoate SL: Cryopreservation of semen in men with testicular tumour or Hodgkin's disease: Results of artificial insemination of their parters. Lancet 1985;2:31–32Google Scholar

Copyright information

© Plenum Publishing Corporation 1998

Authors and Affiliations

  • R. S. Sidhu
    • 1
  • J. Hallak
    • 1
  • R. K. Sharma
    • 1
  • A. J. ThomasJr.
    • 1
  • A. Agarwal
    • 1
  1. 1.Andrology Research and Clinical LaboratoriesDepartment of Urology, The Cleveland Clinic FoundationCleveland

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