, Volume 130, Issue 3, pp 303–313 | Cite as

Sources of resistance to angular leaf spot (Phaeoisariopsis griseola) in common bean core collection, wild Phaseolus vulgaris and secondary gene pool

  • George S. Mahuku
  • Carlos Jara
  • César Cajiao
  • S. Beebe


Angular leaf spot (ALS) is one of the most devastating diseases of common bean (Phaseolus vulgaris L.) in tropical and subtropical countries. The causal fungus, Phaeoisariopsis griseola(Sacc.) Ferr. is highly variable and a diverse source of resistance genes is required to manage this disease. We evaluated a common bean core collection,primary and secondary gene pools and lines derived from inter-specific crosses of P. vulgaris and P. coccineus or P. polyanthus (secondary gene pool) for resistance to angular leaf spot. Of the 1441 accessiones in the core collection, only 2.2% were resistant to both Andean and Mesoamerican races of P. griseola, 28% were resistant only to Andean and 9% to Mesoamerican races. Of the 32 resistant accessions, 68%originated from Bolivia, Colombia,Guatemala and Mexico. More accessions from these countries should be examined for P. griseola reaction. Very few wild P. vulgaris accessions (4%), were resistant to ALS. In contrast, high levels of resistance (62%) were found in the secondary gene pool. Among the 1010 lines from inter-specific crosses, 109 lines were highly resistant. These genotypes from the primary and secondary common bean gene pools resistant to Andean and Mesoamerican races of P. griseola offer a potential for developing broad and durable ALS resistance.

angular leaf spot core collection inter-specific crosses Phaeoisariopsis griseola primary gene pool secondary gene pool 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Beebe, S.E. & M.A. Pastor-Corrales, 1991. Breeding for disease resistance. In: A. van Schoonhoven & O. Voysest (Eds.), Common Beans: Research for Crop Improvement, pp. 561–618.Google Scholar
  2. C.A.B.International, Wallingford, UK and CIAT, Cali, Colombia. Beebe, S.E., P.W. Skroch, J. Tohme, M.C. Duque, F. Pedraza & J. Nienhuis, 2000. Structure of genetic diversity among bean landraces of Middle American origin based on correspondence analysis of RAPD. Crop Sci 40: 264–273.CrossRefGoogle Scholar
  3. Beebe, S., J. Rengifo, E. Gaitan, M.C. Duque, and J. Tohme, 2001. Diversity and origin of Andean landraces of common bean. Crop Sci 41: 854–862.CrossRefGoogle Scholar
  4. Busogoro, J.P., M.H. Jijakli & P. Lepoivre, 1999a. Identification of novel sources of resistance to angular leaf spot disease of common bean within the secondary gene pool. Plant Breed 118: 417–423.CrossRefGoogle Scholar
  5. Busogoro, J.P., M.H. Jijakli & P. Lepoivre, 1999b. Virulence variation and RAPD polymorphism in African isolates of Phaeoisariopsis griseola (Sacc.) Ferr., the causal agent of angular leaf spot of common bean. Eur J Plant Pathol 105: 559–569.CrossRefGoogle Scholar
  6. Chacón, M.I., C. Jara, G. Castellanos, C.E. Posso, R. Buruchara, J.B. Cuasquer & M.A. Pastor-Corrales, 1997. Genetic diversity and relation between common bean angular leaf spot fungus isolates from Africa and South America: genetic improvement implications. Ann Rep Bean Improv Coop 40: 127–128.Google Scholar
  7. CIAT (Centro Internacional de Agricultura Tropical), 1994. Annual Report, Bean Program 1994. CIAT, Cali, Colombia.Google Scholar
  8. CIAT (Centro Internacional de Agricultura Tropical), 1995. Annual Report, Bean Program 1995. CIAT, Cali, Colombia.Google Scholar
  9. Correa Victoria, F.J., 1988. Pathogenic variation, productión of toxic metabolites, and izoenzyme analysis in Phaeoisariopsis griseola (Sacc) Fer. East Lansing, MI, USA: Michigan State University. Ph.D. thesis. 154 p.Google Scholar
  10. Escorcia, B.H. 1998. Caracterization de la diversidad molecular y la virulencia de aislamientos del hongo Phaeoisariopsis griseola de Brasil y Bolivia. Cali, Colombia: Facultad de Ciencias, Universidad del Valle. Ing. thesis. 120 p.Google Scholar
  11. Ferreira, C.F., G.A. Carvalho, S. Nietsche, T.J. Paula Jr, E.G. Barros & M.A. Moreira, 2000. Inheritance of angular leaf spot res313 istance in common bean and identification of a RAPD marker linked to a resistance gene. Crop Sci 40: 1130–1133.CrossRefGoogle Scholar
  12. Gepts, P., 1988a. Phaeolin as an evolution marker. In: P. Gepts (Ed.), Genetic resources of Phaseolus beans, pp. 215-141. Kluwer, Dordrecht, The Netherlands.Google Scholar
  13. Gepts, P., 1988b. A middle American and Andean common bean gene pool. In: P. Gepts (Ed.), Genetic resources of Phaseolus beans, pp. 375-390. Kluwer, Dordrecht, The Netherlands.Google Scholar
  14. Guzmán, P., R.L. Gilbertson, R. Nodari, W.C. Johnson, S.R. Temple, D. Madela, A.B.C. Mkandawire & P. Gepts, 1995. Characterization of variability in the fungus Phaeoisariopsis griseola suggests coevolution with the common bean (Phaseolus vulgaris). Phytopathology 85: 600–607.Google Scholar
  15. Holbrook, C.C., P. Timper & H.Q. Xue, 2000. Evaluation of the core collection aproach for identifying resistance to Meloidogyne arenaria in peanut. Crop Sci 40: 1172–1175.CrossRefGoogle Scholar
  16. Jara, C., G. Castellanos & J.B. Cuasquer, 1998. Determinación de razas en 340 aislamientos de Phaeoisariopsis griseola agente causal de la mancha angular del frijol comÚn (Phaseolus vulgaris) de América y Africa. Resumen En: Memorias XXI Congreso Fitopatología. Ascolfi. Pasto, Colombia, 45.Google Scholar
  17. Liebenberg, M.M. & Z.A. Pretorius, 1997. A review of angular leaf spot of common bean (Phaseolus vulgaris L.). Afr Plant Prot 3: 81–106.Google Scholar
  18. Maya, M., M. Otoya, J. Mayer & M.A. Pastor Corrales, 1995. Marcadores moleculares RAPD confirman la diversidad y evolución de Phaeoisariopsis griseola en América Latina. Fitopatol Colomb 19: 1–6.Google Scholar
  19. Milgroom, M.G. & W.E. Fry, 1997. Contributions of population genetics to plant disease epidemiology and management. Adv Bot Res 24: 1–30.CrossRefGoogle Scholar
  20. McPhee, K.E., A. Tullu, J.M. Kraft & F.J. Muehlbauer, 1999. Resistance to fusarium wilt race 2 in the Pisum core collection. J Am Soc Hort Sci 124: 28–31.Google Scholar
  21. Pachico, D., 1993. The demand for bean technology. Trends in CIAT Commodities. In: G. Henry (Ed.), Working document No. 128, pp. 60-73. CIAT, Cali, Colombia.Google Scholar
  22. Pastor Corrales, M.A. & C. Jara, 1995. La evolución de Phaeoisariopsis griseola con el frijol comÚn en América Latina. Fitopatol Colomb 19: 15–24.Google Scholar
  23. Pastor Corrales, M.A., C. Jara & S.P. Singh, 1998. Pathogenic variation in, source of, and breeding for resistance to Phaeoisariopsis griseola causing angular leaf spot in common bean. Euphytica 103: 161–171.CrossRefGoogle Scholar
  24. Sartorato, A., 2000. Pathogenic variation in Phaeoisariopsis griseola from Brazil. Ann Rep Bean Improv Coop 43: 180–181.Google Scholar
  25. Schoonhoven, A. van & M.A. Pastor-Corrales (comps), 1987. Standard System for the Evaluation of Bean Germplasm. Centro Internacional de Agricultura Tropical, Cali, Colombia.Google Scholar
  26. Schwartz, H.F., M.A. Pastor-Corrales & S.P. Singh, 1982. New sources of resistance to anthracnose and angular leaf spot of beans (Phaseolus vulgaris L.). Euphytica 31: 741–754.CrossRefGoogle Scholar
  27. Simon, C.J. & R.M. Hannan, 1995. Development and use of core subsets of cool-season food legume germplasm collections. HortScience 30: 907.Google Scholar
  28. Trutmann, P. & W. Graf, 1993. The impact of pathogens and arthropods pests in Rwanda. Int J Pest Manag 39: 328–333.CrossRefGoogle Scholar
  29. Singh, S.P., P. Gepts & D.G. Debouck, 1991. Races of common bean (Phaseolus vulgaris L., Fabaceae). Econ Bot 45: 379–396.Google Scholar
  30. Tohme, J., P. Jones, S. Beebe & M. Iwanaga, 1995. The combined use of agroecological and characterization data to establish the CIAT Phaseolus vulgaris core collection. In: T. Hodgkin, A.H.D. Brown, Th. J.L. van Hintum & E.A.V. Morales (Eds.), Core Collection of Plant Resources, pp. 95-107. J Wiley and Sons, Chichester, U.K.Google Scholar
  31. Triana, B., M. Iwanaga, H. Rubiano & M. Andrade, 1993. A study of allogamy in wild Phaseolus vulgaris. Ann Rep Bean Improv Coop 36: 20–21.Google Scholar
  32. Wells, W.C., W.H. Isom & J.G. Waines, 1988. Outcrossing rates of six common bean lines. Crop Sci 28: 177–178.CrossRefGoogle Scholar
  33. Wortmann, C.S., R.A. Kirkby, C.A. Eledu & D.J. Allen, 1998. Atlas of common bean (Phaseolus vulgaris L.) production in Africa. CIAT, Cali, Colombia, pp. 133.Google Scholar

Copyright information

© Kluwer Academic Publishers 2003

Authors and Affiliations

  • George S. Mahuku
    • 1
  • Carlos Jara
    • 1
  • César Cajiao
    • 1
  • S. Beebe
    • 1
  1. 1.Centro Internacional de Agricultura Tropical (CIAT)Cali, ColombiaSouth America

Personalised recommendations