Digestive Diseases and Sciences

, Volume 48, Issue 5, pp 976–985 | Cite as

Decreased Gastric Bacterial Killing and Up-Regulation of Protective Genes in Small Intestine in Gastrin-Deficient Mouse

  • Francis J. Sun
  • Simran Kaur
  • Donna Ziemer
  • Snigdha Banerjee
  • Linda C. Samuelson
  • Robert C. De Lisle
Article

Abstract

Gastrin regulates gastric acid secretion, believed to be primarily responsible for killing ingested microbes. We examined gastric killing of gavaged E. coli in gastrin-deficient mice, which have decreased gastric acid production. Additionally, the expression of intestinal genes involved in epithelial protection were analyzed: the mucus layer glycoprotein muclin, the polymeric Ig receptor, trefoil factor 3, and small proline-rich protein 2a (sprr2a). Gastric pH was 2.5 pH units greater in gastrin-deficient mice, and E. coli survival was increased greater than 20-fold at 10 min after gavage compared to control. Muclin and sprr2a gene expression were significantly increased (2.0- and 2.6-fold) in the intestine, and antibiotic treatment reversed these effects. In conclusion, reduced gastric acid secretion results in increased survival of ingested microorganisms in gastrin-deficient mice. Bacterial survival is associated with increased expression of muclin and sprr2a in the intestine, indicating that these genes play protective roles in the intestine.

gastrin intestine muclin polymeric Ig receptor small proline-rich protein 2a trefoil factor3 

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Copyright information

© Plenum Publishing Corporation 2003

Authors and Affiliations

  • Francis J. Sun
    • 1
  • Simran Kaur
    • 2
  • Donna Ziemer
    • 2
  • Snigdha Banerjee
    • 2
  • Linda C. Samuelson
  • Robert C. De Lisle
    • 2
  1. 1.Laboratory Animal ResourcesMichiganUSA
  2. 2.Department of Anatomy & Cell BiologyUniversity of Kansas Medical CenterKansas City, KansasUSA

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