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Journal of Biomolecular NMR

, Volume 25, Issue 4, pp 349–354 | Cite as

Fast multi-dimensional NMR of proteins

  • Eriks Kupče
  • Ray FreemanEmail author
Article

Abstract

Three-dimensional HNCO and HNCA subspectra from a small protein (agitoxin, 4 kDa, enriched in carbon-13 and nitrogen-15), have been obtained by direct frequency-domain excitation of selected carbon and nitrogen sites. This new technique applies an array of several simultaneous soft radiofrequency spin-inversion pulses, encoded (on or off) according to nested Hadamard matrices, and the resulting responses are extracted by reference to the same matrices. This not only simplifies the spectra but, by avoiding extensive sampling in the traditional evolution dimensions, affords a speed advantage of more than two orders of magnitude.

agitoxin Hadamard HNCA HNCO proteins three-dimensional spectra 

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Copyright information

© Kluwer Academic Publishers 2003

Authors and Affiliations

  1. 1.Varian, Inc, EynshamOxfordU.K
  2. 2.Jesus CollegeCambridgeU.K.

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