Russian Journal of Plant Physiology

, Volume 50, Issue 2, pp 220–223 | Cite as

Plasma Membrane Receptors for Exopolysaccharides of the Ring Rot Causal Agent in Potato Cells

  • T. N. Shafikova
  • A. S. Romanenko
  • G. B. Borovskii


By means of differential centrifugation, microsomal fractions enriched in the plasma membrane were isolated from suspension cell cultures of two cultivars of potato (Solanum tuberosum L.) contrasting in their resistance to the causal agent of ring rot (Clavibacter michiganensis subsp. sepedonicus) (Cms). Electrophoresis of the fractions showed that they comprised a wide range of proteins from 15 to 75 kD. The protein bands were more brightly expressed in the microsomal membranes of the cells of susceptible cultivar. The proteins of 70 and 42 kD were present only in the cellular membranes of the resistant cultivar. In order to visualize the binding of exopolysaccharides (EPS) produced by Cms to the receptors of membrane fractions, a conjugate of EPS with a fluorescent marker was used. The membrane fraction isolated from the cells of the susceptible cultivar was found to be richer in receptors for EPS Cms than the membrane fraction from the resistant cultivar. It is supposed that numerous receptors for EPS present on the plasma membrane may partially account for potato susceptibility to Cms. These receptors may facilitate the binding of bacteria to the plant cells, the formation of colonies, and the development of the disease.

Solanum tuberosum Clavibacter michiganensis subsp. sepedonicus pathogenesis plasma membrane receptors resistance exopolysaccharides 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    D'yakov, Yu.T., Fifty Years of the Theory “Gene-for-Gene”, Usp. Sovrem. Biol., 1996, vol. 116, pp. 293-305.Google Scholar
  2. 2.
    Ozeretskovskaya, O.L. and Romenskaya, M.G., Oligosaccharins as Regulatory Molecules in Plants, Fiziol. Rast. (Moscow), 1996, vol. 43, pp. 743-752 (Russ. J. Plant Physiol., Engl. Transl.).Google Scholar
  3. 3.
    Tarchevsky, I.A., Elicitor-Induced Signaling Pathways and Their Interaction, Fiziol. Rast. (Moscow), 2000, vol. 47, pp. 321-331 (Russ. J. Plant Physiol., Engl. Transl.).Google Scholar
  4. 4.
    Basse, C.W., Fath, A., and Boller, T., High Affinity Binding of a Glycopeptide Elicitor to Tomato Cells and Microsomal Membranes and Displacement by Specific Glucan Suppressor, J. Biol. Chem., 1993, vol. 268, pp. 14724-14731.Google Scholar
  5. 5.
    Nurnberger, T., Wirtz, W., Nennstiel, D., Hahlbrock, K., Jabs, T., Zimmermann, S., and Scheel, D., Signal Perception and Intracellular Signal Transduction in Plant Pathogen Defense, J. Receptor Signal Transduct. Res., 1997, vol. 17, no. 12, pp. 127-136.Google Scholar
  6. 6.
    Klychnikov, O.L., Drabkin, A.V., Vasilenko, O.V., Pavlov, Yu.S., Trofimova, M.S., Smolenskaya, I.N., Rozenkrants, A.A., Sobolev, A.S., and Babakov, A.V., Arrangement of the Fusicoccin Receptor in the Plasma Membrane of Higher Plants: Interrelation between Affinity and Molecular Weight, Biokhimiya, 1998, vol. 63, pp. 1269-1278.Google Scholar
  7. 7.
    Fullone, M.R., Visconti, S., Marra, M., Fogliano, V., and Aducci, P., Fusicoccin Effect on the in vitro Interaction between Plant 14-3-3 Proteins and Plasma Membrane H+-ATPase, J. Biol. Chem., 1998, vol. 273, pp. 7698-7702.Google Scholar
  8. 8.
    Romanenko, A.S., Rymareva, E.V., Kopytchuk, V.N., Ekimova, E.G., and Sobenin, A.M., Characteristics of Exopolysaccharides from the Pathogen Causing Ring Rot and the Sites in Potato Cell Walls with Affinity for Them, Biokhimiya, 1999, vol. 64, pp. 1370-1376.Google Scholar
  9. 9.
    Romanenko, A.S., Rymareva, E.V., Shafikova, T.N., Konenkina, T.A., and Sobenin, A.M., Affinity of Glycoprotein Fraction from Potato Suspension Cells for the Toxin Produced by the Pathogen Causing Ring Rot, Fiziol. Rast. (Moscow), 1997, vol. 44, pp. 887-892 (Russ. J. Plant Physiol., Engl. Transl.).Google Scholar
  10. 10.
    Butenko, R.G., Khromova, L.M., and Sednina, G.V., Metodicheskie ukazaniya po polucheniyu variantnykh kletochnykh linii i rastenii u raznykh sortov kartofelya (Methodological Recommendation on the Selection of Variant Cell Lines and Plants of Various Potato Cultivars), Moscow: Vses. Akad. S-kh. Nauk im. V.I. Lenina, 1984.Google Scholar
  11. 11.
    Korableva, N.P., Ladyzhenskaya, E.P., Lyubimova, N.V., Protsenko, M.A., Kadyrzhanova, D.K., and Metlitsky, L.V., Isolation and Characteristics of the Plasmalemma-Rich Fraction from the Parenchyma of Potato Tubers, Fiziol. Rast. (Moscow), 1980, vol. 27, pp. 1249-1259 (Sov. Plant Physiol., Engl. Transl.).Google Scholar
  12. 12.
    Gayer, G., Ultrahistochemie: Histochemische Arbeitsvorschriften für die Elektronenmikroskopie, Jena: Veb Gustav Fischer, 1973. Translated under the title Electronnaya gistokhimiya Moscow: Mir, 1974.Google Scholar
  13. 13.
    Romanenko, A.S., Graskova, I.A., Rifel', A.A., Kopytchuk, V.N., and Rachenko, M.A., Potato Roots Stabilize the pH of Media Affected by Ring-Rot Causal Agent, Fiziol. Rast. (Moscow), 1996, vol. 43, pp. 707-712 (Russ. J. Plant Physiol., Engl. Transl.).Google Scholar
  14. 14.
    Laemmli, U.K., Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4, Nature, 1970, vol. 227, pp. 680-685.Google Scholar
  15. 15.
    Lomovatskaya, L.A., The Specificity of Infection Development during Early and Final Stages of Pathogenesis Caused by Potato Ring Rot, Cand. Sci. (Biol.) Dissertation, Irkutsk: Sib. Inst. Physiol. Biochem. Plant, Sib. Division. Russ. Acad. Sci., 2001.Google Scholar
  16. 16.
    Aducci, P., Ballio, A., Nastra, D., Fogliano, V., Fullone, M.R., and Marra, M., Fusicoccin and Its Receptors, Plant Hormone Signal Perception and Transduction, Smith, A.R. et al., Eds., Dordrecht: Kluwer, 1996, pp. 141-146.Google Scholar
  17. 17.
    Romanenko, A.S., Rifel', A.A., and Rachenko, M.A., Mechanism for Stabilization of Apoplastic pH Disturbed by Acidic Extracellular Polysaccharides of Ring-Rot Causal Agent in Potato Tissues, Fiziol. Rast. (Moscow), 1998, vol. 45, pp. 833-840 (Russ. J. Plant Physiol., Engl. Transl.).Google Scholar
  18. 18.
    Romanenko, A.S., Rymareva, E.V., Shafikova, T.N., and Salyaev, R.K., The Components of Potato Cell Walls That Posses the Affinity for Exopolysaccharides of Ring-Rot Causal Agent, Dokl. Akad. Nauk, 1998, vol. 358, pp. 277-279.Google Scholar
  19. 19.
    Ozeretskovskaya, O.L., Il'inskaya, L.I., and Vasyukova, N.I., The Mechanisms of Elicitation of Plant Systemic Resistance against Diseases, Fiziol. Rast. (Moscow), 1994, vol. 41, pp. 626-633 (Russ. J. Plant Physiol., Engl. Transl.).Google Scholar
  20. 20.
    Romanenko, A.S., Graskova, I.A., Ekimova, E.G., and Salyaev, R.K., If the “Toxin” of Ring-Rot Causal Agent Exhibits the Properties of Elicitor Inducing Defense Responses?, Dokl. Akad. Nauk, 1997, vol. 355, pp. 844-845.Google Scholar

Copyright information

© MAIK “Nauka/Interperiodica” 2003

Authors and Affiliations

  • T. N. Shafikova
    • 1
  • A. S. Romanenko
    • 1
  • G. B. Borovskii
    • 1
  1. 1.Siberian Institute of Plant Physiology and Biochemistry, iberian DivisionRussian Academy of SciencesIrkutskRussia

Personalised recommendations