Gordonia sinesedis sp. nov., a novel soil isolate
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The taxonomic position of an actinomycete isolated from soil was evaluated using a polyphasic approach. The organism, strain J72, was found to have chemical and morphological properties consistent with its assignment to the genus Gordonia. A nearly complete 16S rDNA sequence of the strain was determined by direct sequencing of the amplified gene. The tested strain formed a distinct phylogenetic line within the evolutionary radiation occupied by the genus Gordonia and was most closely related to G. polyisoprenivorans DSM 44302T. The phenotypic profile of strain 372 readily distinguishes it from representatives of the validly described species of Gordonia. The combined genotypic and phenotypic data show that strain J72 merits recognition as a new species of Gordonia. The name proposed for the new species is Gordonia sinesedis; the type strain is J72T (= DSM 44455T = NCIMB 13802T).
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- Felsenstein J. 1993. PHYLIP (Phylogenetic Inference Package), version 3.5c. Department of Genetics, University of Washington, Seattle, USA.Google Scholar
- Goodfellow M., Mordarski M., Szyba K. and Pulverer G. 1978. Relationship among rhodococci based on deoxyribonucleic acid reasociation. In: Freerksen E., Tárnok I. and Thumim J.H. (eds), Genetics of Actinomycetales. Gustav Fischer Verlag, Stuttgart, pp. 231–234.Google Scholar
- Goodfellow M., Isik K. and Yates E. 1999. Actinomycete systematics: an unfinished synthesis. Nova Acta Leopoldina 312 NF80: 47–82.Google Scholar
- Gordon R.E. and Mihm J.M. 1962. Identification of Nocardia caviae (Erikson) nov.comb. Ann. N.Y. Acad. Sci. 98: 628–636.Google Scholar
- Jukes T.H. and Cantor C.R. 1969. Evolution of protein molecules. In: Munro H.N. (ed.), Mammalian Protein Metabolism Vol. 3. Academic Press, New York, pp. 21–132.Google Scholar
- Kim S.B., Brown R., Oldfield C., Gilbert S.C., Iliarionov S. and Goodfellow M. 2000. Gordonia amicalis sp. nov., a novel dibenzothiophene-desulphurizing actinomycete. Int. J. Syst. Bacteriol. 50: 2031–2036.Google Scholar
- Klatte S., Rainey F.A. and Kroppenstedt R.M. 1994. Transfer of Rhodococcus aichiensis Tsukamura (1982) and Nocardia reviamarae Lechevalier and Lechevalier (1974) to the genus Gordona as Gordona aichiensis comb. nov. and Gordona amarae comb. nov. Int. J. Syst. Bacteriol. 44: 769–773.PubMedGoogle Scholar
- Lechevalier H.A. and Lechevalier M.P. 1970. Chemical composition as a criterion in the classification of aerobic actinomycetes. Int. J. Syst. Bacteriol. 24: 278–288.Google Scholar
- Schaal K.P. 1985. Laboratory diagnosis of actinomycete diseases. In: Goodfellow M. and Minnikin D.E. (eds), Chemical Methods in Bacterial Systematics. Academic Press, London (pp 359–381).Google Scholar
- Stackebrandt E., Smida J. and Collins M.D. 1988. Evidence of phylogenetic heterogeneity within the genus Rhodococcus: revival of the genus Gordona (Tsukamura). J. Gen. Appl. Microbiol. 34: 341–348.Google Scholar
- Wayne L.G., Brenner D.J. and Colwell R.R., and nine other authors 1987. International Committee on Systematic Bacteriology Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int. J. Syst. Bacteriol. 37: 463–464.Google Scholar