Genetica

, Volume 118, Issue 1, pp 69–81

Larval Polytene Chromosomes of Black Flies (Simulium) from Thailand

  • Chaliow Kuvangkadilok
  • Chainarong Boonkemtong
  • Suwannee Phayuhasena
  • Visut Baimai
Article

DOI: 10.1023/A:1022909018987

Cite this article as:
Kuvangkadilok, C., Boonkemtong, C., Phayuhasena, S. et al. Genetica (2003) 118: 69. doi:10.1023/A:1022909018987

Abstract

Larval polytene chromosome maps of Simulium (G.) asakoae and S. (G.) sp. g in the ceylonicum-group and S. (G.) angulistylum, S. (G.) decuplum and S. (G.) siamense in the batoense-group of the subgenus Gomphostilbia from Thailand are presented. These species have three pairs of chromosomes (2n = 6). Light stained centromeric bands were observed in the chromosomes of S. (G.) asakoae, S. (G.) sp. g, S. (G.) decuplum and S. (G.) siamense, whereas heavy dark centromeric bands were present in S. (G.) angulistylum. The best distinguishing character of Simulium species in the subgenus Gomphostilbia is the position of the nucleolar organizer in the short arm of chromosome I. The Ring of Balbiani and the double bubble are located in chromosome arm IIS in all species except for S. (G.) angulistylum, which showed these cytological markers in chromosome arm IIIS. A low chromosomal polymorphism was recorded in all species except for S. (G.) sp. g, which exhibited a standard polytene chromosome. Inversion polymorphisms found in this study conformed to Hardy–Weinberg equilibrium and were not associated with sex. These species have different specific markers and banding patterns although homologous banding sequences were found in chromosome arm IIS in S. (G.) asakoae, S. (G.) sp. g, S. (G.) decuplum and S. (G.) siamense and chromosome arm IIIS in S. (G.) angulistylum. Our results showed no evidence of a sibling species complex within any taxon.

inversion polymorphism polytene chromosome Simulium subgenus Gomphostilbia 

Copyright information

© Kluwer Academic Publishers 2003

Authors and Affiliations

  • Chaliow Kuvangkadilok
    • 1
  • Chainarong Boonkemtong
    • 2
  • Suwannee Phayuhasena
    • 1
  • Visut Baimai
    • 1
  1. 1.Department of Biology, Faculty of ScienceMahidol UniversityBangkokThailand
  2. 2.National Center for Genetic Engineering and BiotechnologyNational Science and Technology Development AgencyBangkokThailand

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