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Chromosome Research

, Volume 11, Issue 2, pp 125–135 | Cite as

Caenorhabditis elegans Ce-rdh-1/rad-51 functions after double-strand break formation of meiotic recombination

  • Takako Takanami
  • Akiyuki Mori
  • Hideyuki Takahashi
  • Saburo Horiuchi
  • Atsushi Higashitani
Article

Abstract

During meiotic prophase 1, homologous recombination is accompanied by dynamic chromosomal changes. The Ce-rdh-1/rad-51 gene is the only bacterial recA-like gene in the nematode C. elegans genome. Upon depletion of Ce-rdh-1/rad-51 using the RNA interference method, abnormal ‘kinked’ chromosomes can be observed in mature oocytes at diakinesis, whereas synapsis between homologous chromosomes during the pachytene stage is normal. Following fertilization, Ce-rdh-1/rad-51-depleted embryos die early in embryogenesis, and their nuclei exhibit abnormal chromosome fragments and bridges. From epistasis analyses with Ce-spo-11 defective mutant and ionizing radiation, it is indicated that Ce-rdh-1/rad-51 functions after double-strand break (DSB) formation of meiotic recombination. Under the Ce-chk-2 defective condition, whose meiotic synapsis and meiotic recombination between homologous chromosomes are completely inhibited, the Ce-rdh-1/rad51 is normally expressed in the gonadal cells. Moreover, it seems that exogenous DSBs in the Ce-chk-2 defective nuclei at the pachytene stage can be repaired between sister chromatids in a Ce-rdh-1/rad-51-dependent manner. These results indicate that Ce-rdh-1/rad51 functions after both endogenous and exogenous DSB formation during meiosis, but not as ‘pairing centers’ for meiotic synapsis.

Ce-chk-2 homologous recombination meiosis recA-like gene 

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Copyright information

© Kluwer Academic Publishers 2003

Authors and Affiliations

  • Takako Takanami
    • 1
    • 2
  • Akiyuki Mori
    • 1
  • Hideyuki Takahashi
    • 1
  • Saburo Horiuchi
    • 2
  • Atsushi Higashitani
    • 1
  1. 1.Graduate School of Life SciencesTohoku UniversitySendaiJapan
  2. 2.School of MedicineIwate Medical UniversityMoriokaJapan

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