Advertisement

Plant Cell, Tissue and Organ Culture

, Volume 73, Issue 1, pp 43–52 | Cite as

Responses of strawberry plantlets cultured in vitro under superbright red and blue light-emitting diodes (LEDs)

  • Duong Tan Nhut
  • T. Takamura
  • H. Watanabe
  • K. Okamoto
  • M. Tanaka
Article

Abstract

Unrooted strawberry cv. `Akihime' shoots with three leaves obtained from standard mixotrophic cultures were cultured in the ``Culture Pack''-rockwool system with sugar-free MS medium under CO2-enriched condition. To examine the effect of superbright red and blue light-emitting diodes (LEDs) on in vitro growth of plantlets, these cultures were placed in an incubator, ``LED PACK'', with either red LEDs, red LEDs1blue LEDs or blue LEDs light source. To clarify the optimum blue and red LED ratio, cultures were placed in ``LED PACK 3'' under LED light source with either 100, 90, 80, or 70% red + 0, 10, 20, 30% blue, respectively, and also under standard heterotrophic conditions. To determine the effects of irradiation level, cultures were grown under 90% red LEDs + 10% blue LEDs at 45, 60 or 75 μmol m−2 s−1 . Plantlet growth was best at 70% red + 30% blue LEDs. The optimal light intensity was 60 μmol m−2 s−1. Growth after transfer to soil was also best after in vitro culture with plantlets produced were 70% red LEDs + 30% blue LEDs.

blue LED ex vitro growth in vitro plantlet growth red LED strawberry 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Appelgren M (1991) Effects of light quality in stem elongation of Pelargonium in vitro. Sci. Hort. 45: 345-351Google Scholar
  2. Barta DJ, Tibbitts TW, Bula RJ & Morrow TW (1992) Evaluation of light-emitting diodes characteristics for a space-based plant irradiation source. Adv. Spa. Res. 12: 141-149Google Scholar
  3. Bertazza G, Baradil R & Predieri S (1995) Light effects on in vitro rooting of pear cultivars of different rhizogenic ability. Plant Cell Tiss. Org. Cult. 41: 139-143Google Scholar
  4. Boxus P (1974) The production of strawberry plants by in vitro micropropagation. J. Hort. Sci. 49:209-210Google Scholar
  5. Brown CS & Schuerger AC (1993) Growth of pepper, lettuce and cucumber under light-emitting diodes. Plant Physiol. (Abstr.) 102:88Google Scholar
  6. Brown CS, Schuerger AC & Sagar JC (1995) Growth and photomorphogenesis of pepper plants under red light-emitting diodes with supplemental blue or far-red light. J. Am. Soc. Hort. Sci. 120: 808-813Google Scholar
  7. Bula RJ, Morrow TW, Tibbitts TW, Barta DJ, Ignatius RW & Martin TS (1991) Light-emitting diodes as a radiation source for plants. HortScience 26: 203-205Google Scholar
  8. Cameron JS, Hancock JF & Nourse T (1985) The field performance of strawberry nursery stock produced originally from runners of micropropagation. Adv. Straw. Prod. 4: 56-58Google Scholar
  9. Cameron JS & Hancock JF (1986) Enhanced vigor in vegetative progeny of micropropagated strawberry plants. HortScience 21: 1225-1226Google Scholar
  10. Chee R (1986) In vitro culture of Vitis: the effects of light spectrum, manganese, and potassium iodide on morphogenesis. Plant Cell Tiss. Org. Cult. 7: 121-134Google Scholar
  11. Chee R & Pool RM (1989) Morphogenetic responses to propagule trimming, spectral irradiance, and photoperiod of grapevine shoots recultured in vitro. HortScience 114: 350-354Google Scholar
  12. Debergh PC, Aitken-Christie J, Cohen D, Grout B, Von Amold S, Zimmerman R & Ziv M (1992) Reconsideration of the term 'vitrification' as used in micropropagation. Plant Cell Tiss. Org. Cult. 30: 135-140Google Scholar
  13. Duncan DB (1995) Multiple range and multiple F test. Biometrics 11: 1-42Google Scholar
  14. Gaba V & Black M (1987) Photoreceptor interaction in plant photomorphogenesis, the limits of experimental techniques and their interpretations. Photochem. Photobiol. 45: 151-156Google Scholar
  15. Hahn EJ, Kozai T & Paek KY (2000) Blue and red light-emitting diodes with or without sucrose and ventilation affects in vitro growth of Rehmannia glutinose plantlets. J. Plant Biol. 43: 247- 250Google Scholar
  16. Hoenecke ME, Bula RJ & Tibbitts TW(1992) Importance of 'blue' photon levels for lettuce seedlings grow under red light-emitting diodes. HortScience 27: 427-430Google Scholar
  17. Hughes KW (1981) In vitro ecology: exogenous factors effecting growth and morphogenesis in plant culture system. Environ. Exp. Bot. 21: 281-288Google Scholar
  18. Langhans RW & Dreesen DR (1988) Challenges to plant growing in space. HortScience 23: 286-293Google Scholar
  19. Lee CG & Palsson BO (1994) High-density algal photobioreactors using light-emitting diodes. Biotech. Bioeng. 44: 1161-1167Google Scholar
  20. McCree KJ (1972) The action spectra, absorptance and quantum yield of photosynthesis in crop plants. Agr. Met. 9: 191-196Google Scholar
  21. Miyashita Y, Kitaya Y, Kozai T & Kimura T (1995) Effects of red and far-red light on the growth and morphology of potato plantlets in vitro: Using light-emitting diodes as a light source for micropropagation. Acta Hort. 393: 710-715Google Scholar
  22. Moreira da Silva MH & Debergh PC (1997) The effect of light quality on the morphogenesis of in vitro cultures of Azorina vidalii (Wats.) Feer. Plant Cell Tiss. Org. Cult. 51: 187-193Google Scholar
  23. Mortensen LM & Stromme E (1987) Effects of light quality on some greenhouse crops. Sci. Hort. 33: 27-36Google Scholar
  24. Murashige T & Skoog F (1962) A revised medium for rapid growth and bioassays with tabacco tissue cultures. Physiol Plant 15: 473-497Google Scholar
  25. Okamoto K & Yanagi T (1994) Development of light source for plant growth using blue and red super-bright LEDs. Shikoku-Section Joint Convention Record of the Institute of Electrical and Related Engineers (pp. 109)Google Scholar
  26. Okamoto K, Yanagi T, Takita S, Tanaka M, Higuchi T & Ushida Y & Watanabe T (1996) Development of growth apparatus using blue and red LED as artificial light source. Acta Hort. 440: 111-116Google Scholar
  27. Rajapakse NC, Pollock RK, McMahon MJ, Kelly JW & Young RE (1992) Interpretation of light quality measurements and plant response in spectral filter research. HortScience 27: 1208-1211Google Scholar
  28. Robin C, Hay MJM & Newton PCD (1994a) Effect of light quality (red:far-red ratio) and defoliation treatments applied at a single phytomer on axillary bud outgrowth in Trifolium repens L. Oecologia 100: 236-242Google Scholar
  29. Robin C, Hay MJM, Newton PCD & Greer (1994b) Effect of light quality (red:far-red ratio) at the apical bud of the main stolon on morphogenesis of Trifolium repens L. Ann. Bot. 72: 119-123Google Scholar
  30. Salisbury FB & Bugbee B (1988) Plant productivity in controlled environments. HortScience 23: 293-299Google Scholar
  31. Sager JC & Wheeler RM (1992) Application of sunlight and lamps for plant irradiation in space bases. Adv. Spa. Res. 12: 133-140Google Scholar
  32. Schuerger AC & Brown CS (1994) Spectral quality may be used to alter plant disease development in CELSS. Adv. Spa. Res. 14: 395-398Google Scholar
  33. Soebo A, Krekling T & Appelgren M (1995) Light quality effects photosynthesis and leaf anatomy of birch plantlets in vitro. Plant Cell Tiss. Org. Cult. 41: 177-185Google Scholar
  34. Sung IK, Kitoya M & Hirano T (1998) The effects of time and intensity of supplemental blue lighting during morning twilight on growth and physiological performance of cucumber seed-lings. Life Supp. Biosph. Sci. 5: 137-142Google Scholar
  35. Swartz HJ, Galletta GJ & Zimmermann RH (1981) Field performance and phenotypic stability of tissue culture-propagated strawberries. J. Am. Soc. Hort. Sci. 106: 667-673Google Scholar
  36. Tennessen DJ, Singsaas EL & Sharkey TD (1994) Light-emitting diodes as a light source for photosynthesis research. Photo. Res. 39: 85-92Google Scholar
  37. Tanaka M (1991) Disposable film culture vessels. In: Bajai YPS (ed) Biotechnology in Agriculture and Forestry, Vol 17 (pp. 212-228). High-Tech and Micropropagation I. Springer-Verlag, BerlinGoogle Scholar
  38. Tanaka M, Goi M & Higashiura T (1988) A novel disposable culture vessel made of fluorocarbon polymer films for micro-propagation. Acta Hort. 226: 663-670Google Scholar
  39. Tanaka M, Nagae S, Takamura T, Kusanagi N, Ujike M & Goi M (1996) Efficiency and application of film culture systems in the in vitro production of plantlets in some horticultural plants. J. Soc. High Tech. Agr. 8: 280-285Google Scholar
  40. Tanaka M, Takamura T, Watanabe H, Endo M, Yanagi T & Okamoto K (1998) In vitro growth of Cymbidium plantlets cultured under superbright red and blue light-emitting diodes (LEDs). J. Hort. Sci. Biotech. 73: 39-44Google Scholar
  41. Tripathy BC & Brown CS (1995) Root-shoot interaction in the greening of wheat seedlings grown under red light. Plant Physiol. 107: 407-411Google Scholar
  42. Yanagi T & Okamoto K (1994) Super-bright light emitting diodes as an artificial light source for plant growth. Abstract of Third International Symposium on Artificial Lighting in Horticulture. Abstract of Third International Symposium on Artificial Lighting in Horticulture (pp. 19)Google Scholar
  43. Wheeler RM, Mackowiak CL & Sager JC (1991) Soybean stem growth under high pressure sodium with supplemental blue lighting. Agr. J. 83: 903-906Google Scholar
  44. Ziv M (1991) Vitrification: morphological and physiological dis-orders of in vitro plants. In: Debergh PC & Zimmerman RH (eds) Micropropagation (pp. 45-69). Kluwer Academic Publisher, DordrechtGoogle Scholar

Copyright information

© Kluwer Academic Publishers 2003

Authors and Affiliations

  • Duong Tan Nhut
    • 1
  • T. Takamura
    • 1
  • H. Watanabe
    • 2
  • K. Okamoto
    • 3
  • M. Tanaka
    • 1
  1. 1.Faculty of AgricultureKagawa UniversityMiki-cho, KagawaJapan
  2. 2.Yokohama Research CenterMitsubishi Chemical Corp.YokohamaJapan
  3. 3.Faculty of EngineeringKagawa University, Hayashi-choTakamatsuJapan

Personalised recommendations