Plant Cell, Tissue and Organ Culture

, Volume 73, Issue 1, pp 43–52 | Cite as

Responses of strawberry plantlets cultured in vitro under superbright red and blue light-emitting diodes (LEDs)

  • Duong Tan Nhut
  • T. Takamura
  • H. Watanabe
  • K. Okamoto
  • M. TanakaEmail author


Unrooted strawberry cv. `Akihime' shoots with three leaves obtained from standard mixotrophic cultures were cultured in the ``Culture Pack''-rockwool system with sugar-free MS medium under CO2-enriched condition. To examine the effect of superbright red and blue light-emitting diodes (LEDs) on in vitro growth of plantlets, these cultures were placed in an incubator, ``LED PACK'', with either red LEDs, red LEDs1blue LEDs or blue LEDs light source. To clarify the optimum blue and red LED ratio, cultures were placed in ``LED PACK 3'' under LED light source with either 100, 90, 80, or 70% red + 0, 10, 20, 30% blue, respectively, and also under standard heterotrophic conditions. To determine the effects of irradiation level, cultures were grown under 90% red LEDs + 10% blue LEDs at 45, 60 or 75 μmol m−2 s−1 . Plantlet growth was best at 70% red + 30% blue LEDs. The optimal light intensity was 60 μmol m−2 s−1. Growth after transfer to soil was also best after in vitro culture with plantlets produced were 70% red LEDs + 30% blue LEDs.

blue LED ex vitro growth in vitro plantlet growth red LED strawberry 


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Copyright information

© Kluwer Academic Publishers 2003

Authors and Affiliations

  • Duong Tan Nhut
    • 1
  • T. Takamura
    • 1
  • H. Watanabe
    • 2
  • K. Okamoto
    • 3
  • M. Tanaka
    • 1
    Email author
  1. 1.Faculty of AgricultureKagawa UniversityMiki-cho, KagawaJapan
  2. 2.Yokohama Research CenterMitsubishi Chemical Corp.YokohamaJapan
  3. 3.Faculty of EngineeringKagawa University, Hayashi-choTakamatsuJapan

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