Clinical & Experimental Metastasis

, Volume 20, Issue 1, pp 51–58

Overexpression of autocrine motility factor receptor (AMFR) in NIH3T3 fibroblasts induces cell transformation

  • Yasuharu Onishi
  • Kazuhiro Tsukada
  • Jun Yokota
  • Avraham Raz
Article

Abstract

Autocrine motility factor receptor (AMFR) is a cell surface glycoprotein of 78000 molecular weight (gp78), regulating cell motility signaling in vitro and metastasis in vivo. To test whether AMFR could be a common mediator of transformation and oncogenic itself, we transfected NIH3T3 fibroblast cells with expression vectors carrying the full-length cDNA for mouse AMFR and evaluated the effects of increased AMFR on transforming potential. The cells stably expressing high levels of AMFR as a result of transfection displayed a complete morphological change and acquired the ability to grow even in low serum. Furthermore, they were anchorage-independent for growth in soft agar and more motile in phagokinetic track assay. Interestingly, the enhanced expression of AMFR produced tumors in nude mice. Our findings provide a direct evidence that overexpression of the AMFR is associated with the acquisition of a transformation phenotype.

motility transformation tumorigenicity phosphohexose isomerase 

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Copyright information

© Kluwer Academic Publishers 2003

Authors and Affiliations

  • Yasuharu Onishi
    • 1
    • 2
  • Kazuhiro Tsukada
    • 2
  • Jun Yokota
    • 3
  • Avraham Raz
    • 1
    • 4
  1. 1.Tumor Progression and Metastasis ProgramKarmanos Cancer InstituteDetroitUSA
  2. 2.Second Department of Surgery, Faculty of MedicineToyama Medical and Pharmaceutical UniversityToyamaJapan
  3. 3.Biology DivisionNational Cancer Center Research InstituteTokyoJapan
  4. 4.Department of Pathology and Radiation OncologyWayne State University School of MedicineDetroitUSA

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